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. 1968 Jan 1;127(1):169–183. doi: 10.1084/jem.127.1.169

IMMUNOGLOBULINS IN NZB/BL MICE

I. SERUM IMMUNOGLOBULIN LEVELS AND IMMUNOGLOBULIN CLASS OF ERYTHROCYTE AUTOANTIBODY

Noel L Warner 1, Richard Wistar Jr 1
PMCID: PMC2138433  PMID: 4169438

Abstract

Determination of serum immunoglobulin levels in NZB and normal mice has indicated that an elevation of the γM-globulin level occurs in NZB mice. This can be demonstrated in young NZB mice well before other autoimmune manifestations are observed. A slight elevation of γM-globulin was also observed in NZB hybrid mice. Erythrocyte autoantibodies were analyzed by the direct Coombs' test with specific antiimmunoglobulin reagents. Autoantibodies in NZB mice can be of all immunoglobulin classes, although predominantly of γG1-class. In (NZB x NZC)F1 hybrid mice, although Coombs' positivity develops around the same age as in NZB mice, there is a greater restriction of the autoantibodies into γM- and γG1-classes. Preliminary attempts to quantitate immunoglobulins coating NZB red cells have shown that there are approximately 400 molecules of γM and 5000 molecules of γG-globulin per NZB mouse red cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIELSCHOWSKY M., BIELSCHOWSKY F. OBSERVATIONS ON NZB/B1 MICE; DIFFERENTIAL FERTILITY IN RECIPROCAL CROSSES AND THE TRANSMISSION OF THE AUTO-IMMUNE HAEMOLYTIC ANAEMIA TO NZB/B1 X NZC/B1 HYBRIDS. Aust J Exp Biol Med Sci. 1964 Aug;42:561–568. [PubMed] [Google Scholar]
  2. Barth W. F., McLaughlin C. L., Fahey J. L. The immunoglobulins of mice. VI. Response to immunization. J Immunol. 1965 Nov;95(5):781–790. [PubMed] [Google Scholar]
  3. Burnet F. M., Holmes M. C. The natural history of the NZB/NZW F1 hybrid mouse: a laboratory model of systemic lupus erythematosus. Australas Ann Med. 1965 Aug;14(3):185–191. doi: 10.1111/imj.1965.14.3.185. [DOI] [PubMed] [Google Scholar]
  4. Burnet M., Holmes M. C. Genetic investigations of autoimmune disease in mice. Nature. 1965 Jul 24;207(995):368–371. doi: 10.1038/207368a0. [DOI] [PubMed] [Google Scholar]
  5. Coe J. E. 7Sgamma-1 and 7Sgamma-2 antibody response in the mouse. I. Influence of strain, antigen and adjuvant. J Immunol. 1966 Apr;96(4):744–753. [PubMed] [Google Scholar]
  6. Diener E. The immune response in NZB and NZB x C3H F1-hybrid mice as measured by the haemolysin plaque technique. Int Arch Allergy Appl Immunol. 1966;30(2):120–131. doi: 10.1159/000229801. [DOI] [PubMed] [Google Scholar]
  7. East J., De Sousa M. A. The thymus autoimmunity and malignancy in New Zealand black mice. Natl Cancer Inst Monogr. 1966 Sep;22:605–616. [PubMed] [Google Scholar]
  8. East J., de Sousa M. A., Parrott D. M. Immunopathology of New Zealand black (NZB) mice. Transplantation. 1965 Nov;3(6):711–729. doi: 10.1097/00007890-196511000-00003. [DOI] [PubMed] [Google Scholar]
  9. FAHEY F. L., LAWRENCE M. E. QUANTITATIVE DETERMINATION OF 6.6 S GAMMA-GLOBULINS, BETA-2A-GLOBULINS AND GAMMA-1-MACROGLOBULINS IN HUMAN SERUM. J Immunol. 1963 Nov;91:597–603. [PubMed] [Google Scholar]
  10. FINK M. A., QUINN V. A. Antibody production in inbred strains of mice. J Immunol. 1953 Jan;70(1):61–67. [PubMed] [Google Scholar]
  11. FUDENBERG H., GERMAN J. L., 3rd, KUNKEL H. G. The occurrence of rheumatoid factor and other abnormalities in families of patients with agammaglobulinemia. Arthritis Rheum. 1962 Dec;5:565–588. doi: 10.1002/art.1780050605. [DOI] [PubMed] [Google Scholar]
  12. GREENBURY C. L., MOORE D. H., NUNN L. A. REACTION OF 7S AND 19S COMPONENTS OF IMMUNE RABBIT ANTISERA WITH HUMAN GROUP A AND AB RED CELLS. Immunology. 1963 Sep;6:421–433. [PMC free article] [PubMed] [Google Scholar]
  13. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Giltinan P. J., Norins L. C., Holmes M. C. Relation of reticulocytosis to positive Coombs tests in NZB and other mice. Blood. 1965 Dec;26(6):790–797. [PubMed] [Google Scholar]
  15. HELYER B. J., HOWIE J. B. Renal disease associated with positive lupus erythematosus tests in a cross-bred strain of mice. Nature. 1963 Jan 12;197:197–197. doi: 10.1038/197197a0. [DOI] [PubMed] [Google Scholar]
  16. HELYER B. J., HOWIE J. B. Spontaneous auto-immune disease in NZB/BL mice. Br J Haematol. 1963 Apr;9:119–131. doi: 10.1111/j.1365-2141.1963.tb05450.x. [DOI] [PubMed] [Google Scholar]
  17. HENSON J. B., GORHAM J. R., LEADER R. W., WAGNER B. M. Experimental hypergammaglobulinemia in mink. J Exp Med. 1962 Sep 1;116:357–364. doi: 10.1084/jem.116.3.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. HERZENBERG L. A., WARNER N. L., HERZENBERG L. A. IMMUNOGLOBULIN ISOANTIGENS (ALLOTYPES) IN THE MOUSE. I. GENETICS AND CROSS-REACTIONS OF THE 7S GAMMA-2A-ISOANTIGENS CONTROLLED BY ALLELES AT THE IG-1 LOCUS. J Exp Med. 1965 Mar 1;121:415–438. doi: 10.1084/jem.121.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. HOLMES M. C., BURNET F. M. THE INHERITANCE OF AUTOIMMUNE DISEASE IN MICE: A STUDY OF HYBRIDS OF THE STRAINS NZB AND C3H. Heredity (Edinb) 1964 Aug;19:419–434. doi: 10.1038/hdy.1964.52. [DOI] [PubMed] [Google Scholar]
  20. HOLMES M. C., BURNET F. M. THE NATURAL HISTORY OF AUTOIMMUNE DISEASE IN NZB MICE. A COMPARISON WITH THE PATTERN OF HUMAN AUTOIMMUNE MANIFESTATIONS. Ann Intern Med. 1963 Sep;59:265–276. doi: 10.7326/0003-4819-59-3-265. [DOI] [PubMed] [Google Scholar]
  21. Kamm D. P., Miyasato F., Pollak V. E. Serum proteins in NZB/Bl and related strains of mice. Proc Soc Exp Biol Med. 1966 Jul;122(3):903–906. doi: 10.3181/00379727-122-31285. [DOI] [PubMed] [Google Scholar]
  22. Kember N. F. An in vivo cell survival system based on the recovery of rat growth cartilage from radiation injury. Nature. 1965 Jul 31;207(996):501–503. doi: 10.1038/207501a0. [DOI] [PubMed] [Google Scholar]
  23. LEDDY J. P., BAKEMEIER R. F. STRUCTURAL ASPECTS OF HUMAN ERYTHROCYTE AUTOANTIBODIES. I. L CHAIN TYPES AND ELECTROPHORETIC DISPERSION. J Exp Med. 1965 Jan 1;121:1–17. doi: 10.1084/jem.121.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. LONG G., HOLMES M. C., BURNET F. M. AUTOANTIBODIES PRODUCED AGAINST MOUSE ERYTHROCYTES IN NZB MICE. Aust J Exp Biol Med Sci. 1963 Aug;41:315–322. doi: 10.1038/icb.1963.31. [DOI] [PubMed] [Google Scholar]
  25. MELLORS R. C. AUTOIMMUNE DISEASE IN NZB/BL MICE. I. PATHOLOGY AND PATHOGENESIS OF A MODEL SYSTEM OF SPONTANEOUS GLOMERULONEPHRITIS. J Exp Med. 1965 Jul 1;122:25–40. doi: 10.1084/jem.122.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Mellors R. C. Autoimmune disease in NZB-Bl mice. II. Autoimmunity and malignant lymphoma. Blood. 1966 Apr;27(4):435–448. [PubMed] [Google Scholar]
  27. Minna J. D., Iverson G. M., Herzenberg L. A. Identification of a gene locus for gamma-G-1 immunoglobulin H chains and its linkage to the H chain chromosome region in the mouse. Proc Natl Acad Sci U S A. 1967 Jul;58(1):188–194. doi: 10.1073/pnas.58.1.188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. NORINS L. C., HOLMES M. C. ANTINUCLEAR FACTOR IN MICE. J Immunol. 1964 Jul;93:148–154. [PubMed] [Google Scholar]
  29. NORINS L. C., HOLMES M. C. GLOBULINS OF NZB MOUSE ERYTHROCYTES. J Immunol. 1964 Dec;93:897–901. [PubMed] [Google Scholar]
  30. NOSSAL G. J., SZENBERG A., ADA G. L., AUSTIN C. M. SINGLE CELL STUDIES ON 19S ANTIBODY PRODUCTION. J Exp Med. 1964 Mar 1;119:485–502. doi: 10.1084/jem.119.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. NUSSENZWEIG R. S., MERRYMAN C., BENACERRAF B. ELECTROPHORETIC SEPARATION AND PROPERTIES OF MOUSE ANTIHAPTEN ANTIBODIES INVOLVED IN PASSIVE CUTANEOUS ANAPHYLAXIS AND PASSIVE HEMOLYSIS. J Exp Med. 1964 Aug 1;120:315–328. doi: 10.1084/jem.120.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. OVARY Z., BARTH W. F., FAHEY J. L. THE IMMUNOGLOBULINS OF MICE. 3. SKIN SENSITIZING ACTIVITY OF MOUSE IMMUNOGLOBULINS. J Immunol. 1965 Mar;94:410–415. [PubMed] [Google Scholar]
  33. POTTER M., KUFF E. L. DISORDERS IN THE DIFFERENTIATON OF PROTEIN SECRETION IN NEOPLASTIC PLASMA CELLS. J Mol Biol. 1964 Aug;9:537–544. doi: 10.1016/s0022-2836(64)80225-4. [DOI] [PubMed] [Google Scholar]
  34. Potter M., Appella E., Geisser S. Variations in the heavy polypeptide chain structure of gamma myeloma immunoglobulins from an inbred strain of mice and a hypothesis as to their origin. J Mol Biol. 1965 Dec;14(2):361–372. doi: 10.1016/s0022-2836(65)80187-5. [DOI] [PubMed] [Google Scholar]
  35. ROITT I. M., CAMPBELL P. N., DONIACH D. The nature of the thyroid auto-antibodies present in patients with Hashimoto's thyroiditis (lymphadenoid goitre). Biochem J. 1958 Jun;69(2):248–256. doi: 10.1042/bj0690248. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Seligmann M., Danon F., Mihaesco C., Fudenberg H. H. Immunoglobulin abnormalities in families of patients with Waldenström's macroglobulinemia. Am J Med. 1967 Jul;43(1):66–83. doi: 10.1016/0002-9343(67)90149-0. [DOI] [PubMed] [Google Scholar]
  37. Siegler R. PATHOGENESIS OF THYMIC CHANGES IN NZB MICE WITH HEMOLYTIC ANEMIA. J Exp Med. 1965 Oct 31;122(5):929–942. doi: 10.1084/jem.122.5.929. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. WEILER R. J., HOFSTRA D., SZENTIVANYI A., BLAISDELL R., TALMAGE D. W. The inhibition of labeled antigen precipitation as a measure of serum gamma-globulin. J Immunol. 1960 Aug;85:130–137. [PubMed] [Google Scholar]
  39. Warner N. L., Herzenberg L. A., Goldstein G. Immunoglobulin isoantigens (allotypes) in the mouse. II. Allotypic analysis of three gammaG2-myeloma proteins from (NZB x BALB/c)F1 hybrids and of normal gammaG2-globulins. J Exp Med. 1966 Apr 1;123(4):707–721. doi: 10.1084/jem.123.4.707. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Warner N. L., Herzenberg L. A. Immunoglobulin isoantigens (allotypes) in the mouse. 3. Detection of allotypic antigens with heterologous antisera. J Immunol. 1966 Oct;97(4):525–531. [PubMed] [Google Scholar]
  41. Yount W. J., Kunkel H. G., Litwin S. D. Studies of the Vi (gamma-2c) subgroup of gamma-globulin. A relationship between concentration and genetic type among normal individuals. J Exp Med. 1967 Jan 1;125(1):177–190. doi: 10.1084/jem.125.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. ZINGALE S. B., SANCHEZAVALOS J. C., ANDRADA J. A., STRINGA S. G., MANNI J. A. APPEARANCE OF ANTICOAGULANT FACTORS AND CERTAIN "AUTOIMMUNE" ANTIBODIES FOLLOWING ANTIGENIC STIMULATION WITH BLOOD GROUP SUBSTANCES IN PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS. Arthritis Rheum. 1963 Oct;6:581–598. doi: 10.1002/art.1780060503. [DOI] [PubMed] [Google Scholar]
  43. de Vries M. J., Hijmans W. Pathological changes of thymic epithelial cells and autoimmune disease in NZB, NZW and (NZB x NZW)F1 mice. Immunology. 1967 Feb;12(2):179–196. [PMC free article] [PubMed] [Google Scholar]

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