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. 1968 Jan 31;127(2):277–290. doi: 10.1084/jem.127.2.277

ANTIGENS IN IMMUNITY

XV. ULTRASTRUCTURAL FEATURES OF ANTIGEN CAPTURE IN PRIMARY AND SECONDARY LYMPHOID FOLLICLES

G J V Nossal 1, A Abbot 1, Judith Mitchell 1, Z Lummus 1
PMCID: PMC2138444  PMID: 4169585

Abstract

This paper describes the trapping of antigen in lymphoid follicles of rat popliteal lymph nodes as revealed by electron microscopic radioautographs following injection of 125I-labeled Salmonella adelaide flagella and other materials. The antigen was taken up vigorously, and to an approximately equal extent, by both primary and secondary follicles. The rate of uptake was faster in preimmunized than in virgin adult rats. The bulk of the antigen in follicles was extracellular, and persisted in this location for at least 3 wk. Label was most frequently found at or near the surface of fine cell processes. Many of these were branches of dendritic follicular reticular cells. Such processes interdigitated with equally fine processes of lymphocytes, creating an elaborate meshwork. In some cases, antigen was found between lymphocytes which appeared to be in close apposition. Occasionally, a few grains appeared over lymphocyte nuclei and study of serial sections suggested that this probably represented true entry of small amounts of antigen into lymphocytes. The characteristic "tingible body" macrophages (TBM) of germinal centers appeared to play only a secondary role in follicular antigen retention. They showed degrees of labeling over their phagocytic inclusions varying from negligible to moderately heavy. Moreover, follicles lacking or poor in TBM retained antigen just as effectively as those containing numerous TBM. The hypothesis is advanced that TBM may be derived from monocytes that migrate down from the circular sinus. Follicular localization of three other materials was also studied, though not in such detail. These were 125I-HSA complexed to anti-HSA: 125I-labeled autologous IgG; and 125I-monomeric flagellin. All of these showed the basic features of intercellular, membrane-associated deposition noted with 125I-flagella. The role of follicular antigen depots in immune induction is discussed. The tentative conclusion is reached that follicular antigen in a primary follicle encounters natural antibody on the surface of certain antigen-reactive lymphocytes. The resultant reaction causes blast cell transformation and eventually the genesis of a germinal center.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADA G. L., NOSSAL G. J., AUSTIN C. M. ANTIGENS IN IMMUNITY. V. THE ABILITY OF CELLS IN LYMPHOID FOLLICLES TO RECOGNIZE FOREIGNNESS. Aust J Exp Biol Med Sci. 1964 Jun;42:331–346. [PubMed] [Google Scholar]
  2. CLARK S. L., Jr The reticulum of lymph nodes in mice studied with the electron microscope. Am J Anat. 1962 May;110:217–257. doi: 10.1002/aja.1001100303. [DOI] [PubMed] [Google Scholar]
  3. Curtain C. C., Baumgarten A. Immunocytochemical localization of the immunoglobulin factors Gm(a), Gm(b) and Inv(a) in human lymphoid tissue. Immunology. 1966 Jun;10(6):499–506. [PMC free article] [PubMed] [Google Scholar]
  4. MELLORS R. C., KORNGOLD L. THE CELLULAR ORIGIN OF HUMAN IMMUNOGLOBULINS (GAMMA-2, GAMMA-1M, GAMMA-1A). J Exp Med. 1963 Sep 1;118:387–396. doi: 10.1084/jem.118.3.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MILLER J. J., 3rd, NOSSAL G. J. ANTIGENS IN IMMUNITY. VI. THE PHAGOCYTIC RETICULUM OF LYMPH NODE FOLLICLES. J Exp Med. 1964 Dec 1;120:1075–1086. doi: 10.1084/jem.120.6.1075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mitchell J., Abbot A. Ultrastructure of the antigen-retaining reticulum of lymph node follicles as shown by high-resolution autoradiography. Nature. 1965 Oct 30;208(5009):500–502. doi: 10.1038/208500b0. [DOI] [PubMed] [Google Scholar]
  7. NOSSAL G. J., ADA G. L., AUSTIN C. M. ANTIGENS IN IMMUNITY. IV. CELLULAR LOCALIZATION OF 125-I- AND 131-I-LABELLED FLAGELLA IN LYMPH NODES. Aust J Exp Biol Med Sci. 1964 Jun;42:311–330. [PubMed] [Google Scholar]
  8. Nossal G. J., Abbot A., Mitchell J. Antigens in immunity. XIV. Electron microscopic radioautographic studies of antigen capture in the lymph node medulla. J Exp Med. 1968 Feb 1;127(2):263–276. doi: 10.1084/jem.127.2.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Nossal G. J., Ada G. L., Austin C. M., Pye J. Antigens in immunity. 8. Localization of 125-I-labelled antigens in the secondary response. Immunology. 1965 Oct;9(4):349–357. [PMC free article] [PubMed] [Google Scholar]
  10. Nossal G. J., Austin C. M., Pye J., Mitchell J. Antigens in immunity. XII. Antigen trapping in the spleen. Int Arch Allergy Appl Immunol. 1966;29(4):368–383. [PubMed] [Google Scholar]
  11. Nossal G. J. Mechanisms of antibody production. Annu Rev Med. 1967;18:81–96. doi: 10.1146/annurev.me.18.020167.000501. [DOI] [PubMed] [Google Scholar]
  12. Robinson W. A., Marbrook J., Diener E. Primary stimulation and measurement of antibody production to sheep red blood cells in vitro. J Exp Med. 1967 Aug 1;126(2):347–356. doi: 10.1084/jem.126.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SELL S., GELL P. G. STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM. J Exp Med. 1965 Aug 1;122:423–440. doi: 10.1084/jem.122.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Swartzendruber D. C. Desmosomes in germinal centers of mouse spleen. Exp Cell Res. 1965 Nov;40(2):429–432. doi: 10.1016/0014-4827(65)90277-6. [DOI] [PubMed] [Google Scholar]
  15. Swartzendruber D. C., Hanna M. G., Jr Electron microscopic autoradiography of germinal center cells in mouse spleen. J Cell Biol. 1965 Jun;25(3 Suppl):109–119. doi: 10.1083/jcb.25.3.109. [DOI] [PubMed] [Google Scholar]
  16. Williams G. M. Antigen localization in lymphopenic states. II. Further studies on whole body x-irradiation. Immunology. 1966 Nov;11(5):475–488. [PMC free article] [PubMed] [Google Scholar]

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