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. 1968 Jan 31;127(2):341–357. doi: 10.1084/jem.127.2.341

STUDIES ON THE PATHOGENESIS OF FEVER

XVI. PURIFICATION AND FURTHER CHEMICAL CHARACTERIZATION OF GRANULOCYTIC PYROGEN

Marilyn Sue Kozak 1, Helmut H Hahn 1, William J Lennarz 1, W Barry Wood Jr 1
PMCID: PMC2138451  PMID: 5635382

Abstract

Small quantities of highly purified granulocytic pyrogen have been separated from contaminating proteins by disc electrophoresis in polyacrylamide gel. The biologically active material thus isolated was shown to be electrophoretically homogeneous at pH 9 and pH 3.8. Earlier work on the chemical properties of the pyrogen molecule has been extended to include: (a) estimation of its molecular weight by gel filtration; (b) demonstration of free sulfhydryl groups essential for its biological activity; and (c) evidence that it is not inactivated by exhaustive extraction with ethanolether or n-heptane.

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Selected References

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