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. 1968 Feb 29;127(3):589–603. doi: 10.1084/jem.127.3.589

HETEROGENEITY OF RABBIT IGM ANTIBODY AS DETECTED BY C'1a FIXATION

Leon W Hoyer 1, Tibor Borsos 1, Herbert J Rapp 1, Wilton E Vannier 1
PMCID: PMC2138465  PMID: 4169967

Abstract

The C'1a-fixing properties of purified rabbit IgM anti-benzenearsonate antibody were determined. When tested with sheep erythrocytes to which hapten had been coupled by diazo linkage, the number of C'1a molecules fixed was 21% of the number of IgM antibody molecules bound to the erythrocyte surface. This was not due to loss of C'1a-fixing capacity during the purification procedure. Preparative electrophoresis of the antibody concentrated C'1a-fixing molecules in the anodal region so that antibody fractions with greater C'1a-fixing capacity were obtained. The demonstration that C'1a fixation is a property of a subpopulation of IgM molecules provides evidence for previously unrecognized µ-chain heterogeneity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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