Abstract
The effects of a single injection of a bacterial endotoxin on the cellular changes of a primary immune response to a standard dose of sheep red blood cells were studied in the spleens and mesenteric lymph nodes of mice. Daily histological comparisons of these organs in mice, injected with endotoxin, or with antigen, or both, showed that endotoxin given simultaneously with sheep red blood cells, as antigen, significantly enhanced all of the cellular changes that appear in the mesenteric lymph nodes and spleens of mice that form antibody when that antigen is given alone. First, in the white pulp of the spleens and cortical regions of the nodes, there appeared an early and excessive proliferation of the large pyroninophilic cells which seems to be responsible for the earliest formation of antibody, as judged by this work and that of others cited in the body of the paper. Polymorphonuclear cells invaded the spleens of these animals early after simultaneous challenge with antigen and endotoxin, and in far greater numbers than have ever been seen in mice given the same antigen without endotoxin. "Activated" germinal centers formed in the lymphoid tissue either 1 day before the appearance of antibody in the blood stream or on the same day, and they became larger than in the mice given antigen only. On the other hand, these specific and characteristic cellular changes failed to appear in mice prevented from forming any antibody at all by injections of endotoxin given 2 days before the antigenic challenge. These findings are discussed in the light provided by data from recent reports of others as well as in the light of the accompanying paper (1) which demonstrated not only the enhancement of antibody formation following simultaneous injections of antigen and endotoxin, as already known, but a totally unexpected, complete suppression of its formation when endotoxin was given 2 days before antigen.
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Selected References
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- ADA G. L., NOSSAL G. J., AUSTIN C. M. ANTIGENS IN IMMUNITY. V. THE ABILITY OF CELLS IN LYMPHOID FOLLICLES TO RECOGNIZE FOREIGNNESS. Aust J Exp Biol Med Sci. 1964 Jun;42:331–346. [PubMed] [Google Scholar]
- CONGDON C. C., MAKINODAN T. Splenic white pulp alteration after antigen injection: relation to time of serum antibody production. Am J Pathol. 1961 Dec;39:697–709. [PMC free article] [PubMed] [Google Scholar]
- Franzl R. E., McMaster P. D. The primary immune response in mice. I. The enhancement and suppression of hemolysin production by a bacterial endotoxin. J Exp Med. 1968 Jun 1;127(6):1087–1107. doi: 10.1084/jem.127.6.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOWANS J. L., McGREGOR D. D., COWEN D. M. Initiation of immune responses by small lymphocytes. Nature. 1962 Nov 17;196:651–655. doi: 10.1038/196651a0. [DOI] [PubMed] [Google Scholar]
- GOWANS J. L. The fate of parental strain small lymphocytes in F1 hybrid rats. Ann N Y Acad Sci. 1962 Oct 24;99:432–455. doi: 10.1111/j.1749-6632.1962.tb45326.x. [DOI] [PubMed] [Google Scholar]
- HANNA M. G., Jr GERMINAL CENTER CHANGES AND PLASMA CELL REACTION DURING THE PRIMARY IMMUNE RESPONSE. Int Arch Allergy Appl Immunol. 1965;26:230–251. doi: 10.1159/000229574. [DOI] [PubMed] [Google Scholar]
- KEUNING F. J., van der MEER, NIEUWENHUIS P., OUDENDIJK P. The histophysiology of the antibody response. II. Antibody responses and splenic plasma cell reaction in sublethally x-irradiated rabbits. Lab Invest. 1963 Feb;12:156–170. [PubMed] [Google Scholar]
- LANGEVOORT H. L. The histophysiology of the antibody response. I. Histogenesis of the plasma cell reaction in rabbit spleen. Lab Invest. 1963 Jan;12:106–118. [PubMed] [Google Scholar]
- NOSSAL G. J., ADA G. L., AUSTIN C. M. ANTIGENS IN IMMUNITY. IV. CELLULAR LOCALIZATION OF 125-I- AND 131-I-LABELLED FLAGELLA IN LYMPH NODES. Aust J Exp Biol Med Sci. 1964 Jun;42:311–330. [PubMed] [Google Scholar]
- SCOTHORNE R. J., MCGREGOR I. A. Cellular changes in lymph nodes and spleen following skin homografting in the rabbit. J Anat. 1955 Jul;89(3):283–292. [PMC free article] [PubMed] [Google Scholar]
- THORBECKE G. J., ASOFSKY R. M., HOCHWALD G. M., SISKIND G. W. Gamma globulin and antibody formation in vitro. III. Induction of secondary response at different intervals after the primary; the role of secondary nodules in the preparation for the secondary response. J Exp Med. 1962 Sep 1;116:295–310. doi: 10.1084/jem.116.3.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WARD P. A., JOHNSON A. G., ABELL M. R. Studies on the adjuvant action of bacterial endotoxins on antibody formation. III. Histologic response of the rabbit spleen to a single injection of a purified protein antigen. J Exp Med. 1959 May 1;109(5):463–474. doi: 10.1084/jem.109.5.463. [DOI] [PMC free article] [PubMed] [Google Scholar]