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. 1968 Jul 1;128(1):223–233. doi: 10.1084/jem.128.1.223

STUDIES ON THE COMPETENCE OF SINGLE CELLS TO PRODUCE ANTIBODIES OF TWO SPECIFICITIES

Harriet Gershon 1, Sara Bauminger 1, Michael Sela 1, Michael Feldman 1
PMCID: PMC2138506  PMID: 5662016

Abstract

Two series of experiments were performed, utilizing a modification of the hemolysin plaque technique which registers 19S antibody, in an attempt to determine the frequency of cells capable of simultaneously producing antibody to two non-cross-reacting antigens. Mice were immunized i.v. with rabbit and camel RBC and their spleens assayed for cells producing antibody against both antigens. 16,904 cells producing antibody of one or the other specificity, from 26 mice, were counted. Not one cell was detected which produced antibody of two specificities. Rabbits were immunized intradermally with HSA to which polyalanyl and p-azobenzenearsonate groups were chemically attached. The individual haptens, polyalanyl, and p-azobenzenearsonate groups were coupled to separate aliquots of SRBC, and the lymph nodes of immunized rabbits were assayed for cells releasing antibody against both haptens. In a study of 11 rabbits, after counting 27,845 cells producing antibody, we detected no "double" plaques.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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