Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1968 Aug 1;128(2):293–307. doi: 10.1084/jem.128.2.293

CYTOLOGY OF IMMUNOLOGIC MEMORY

A MORPHOLOGIC STUDY OF LYMPHOID CELLS DURING THE ANAMNESTIC RESPONSE

Cesare Bosman 1, Joseph D Feldman 1
PMCID: PMC2138521  PMID: 5659523

Abstract

Pairs of rats were immunized with keyhole limpet hemocyanin (KLH) and simultaneously labeled with thymidine-methyl-3H or 5-iodo-2'-deoxyuridine-125I. From 10–50 days later, their lymphoid organs were examined 3 days after anamnestic stimulation with KLH or after primary injection of BGG. Light and electron microscopic study of the labeled cells revealed that immunologic memory resided in the mature resting monoribosomal lymphocyte which, upon stimulation, transformed to an immature polyribosomal lymphocyte and mitotically active blast cell. These latter elements differentiated into plasma cells directly or after mitosis.

Full Text

The Full Text of this article is available as a PDF (1,004.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALFOUR B. M., COOPER E. H., ALPEN E. L. MORPHOLOGICAL AND KINETIC STUDIES ON ANTIBODY-PRODUCING CELLS IN RAT LYMPH NODES. Immunology. 1965 Mar;8:230–244. [PMC free article] [PubMed] [Google Scholar]
  2. BRYANT B. J. Reutilization of leukocyte DNA by cells of regenerating liver. Exp Cell Res. 1962 Jun;27:70–79. doi: 10.1016/0014-4827(62)90044-7. [DOI] [PubMed] [Google Scholar]
  3. CAFFREY R. W., RIEKE W. O., EVERETT N. B. Radioautographic studies of small lymphocytes in the thoracic duct of the rat. Acta Haematol. 1962;28:145–154. doi: 10.1159/000207257. [DOI] [PubMed] [Google Scholar]
  4. CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CRADDOCK C. G., NAKAI G. S., FUKUTA H., VANSLAGER L. M. PROLIFERATIVE ACTIVITY OF THE LYMPHATIC TISSUES OF RATS AS STUDIED WITH TRITIUM-LABELED THYMIDINE. J Exp Med. 1964 Sep 1;120:389–412. doi: 10.1084/jem.120.3.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Celada F. Quantitative studies of the adoptive immunological memory in mice. II. Linear transmission of cellular memory. J Exp Med. 1967 Feb 1;125(2):199–211. doi: 10.1084/jem.125.2.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DIDERHOLM H., FICHTELIUS K. E., LINDER O. Availability time of 3H-label after administration of 3H-thymidine in vivo. Exp Cell Res. 1962 Sep;27:431–435. doi: 10.1016/0014-4827(62)90008-3. [DOI] [PubMed] [Google Scholar]
  8. EIDINOFF M. L., CHEONG L., GURPIDE E. G., BENUA R. S., ELLISON R. R. Incorporation of 5-iodouracil labelled with iodine-131 into the deoxyribonucleic acid of human leukaemic leucocytes following in vivo administration of 5-iododeoxyuridine labelled with iodine-131. Nature. 1959 Jun 13;183(4676):1686–1687. doi: 10.1038/1831686a0. [DOI] [PubMed] [Google Scholar]
  9. Feinendegen L. E., Bond V. P., Hughes W. L. Physiological thymidine reutilization in rat bone marrow. Proc Soc Exp Biol Med. 1966 Jun;122(2):448–455. doi: 10.3181/00379727-122-31159. [DOI] [PubMed] [Google Scholar]
  10. Feldman J. D., Nordquist R. E. Immunologic competence of thoracic duct cells. II. Ultrastructure. Lab Invest. 1967 Apr;16(4):564–579. [PubMed] [Google Scholar]
  11. Gowans J. L., Uhr J. W. The carriage of immunological memory by small lymphocytes in the rat. J Exp Med. 1966 Nov 1;124(5):1017–1030. doi: 10.1084/jem.124.5.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HILL M. Intercellular passage of tritium-thymidine-labeled DNA from donor lymphocytes to recipient bone marrow cells. Exp Cell Res. 1962 Oct;28:21–26. doi: 10.1016/0014-4827(62)90308-7. [DOI] [PubMed] [Google Scholar]
  13. HUGHES W. L., COMMERFORD S. L., GITLIN D., KRUEGER R. C., SCHULTZE B., SHAH V., REILLY P. DEOXYRIBONUCLEIC ACID METABOLISM IN VIVO: I. CELL PROLIFERATION AND DEATH AS MEASURED BY INCORPORATION AND ELIMINATION OF IODODEOXYURIDINE. Fed Proc. 1964 May-Jun;23:640–648. [PubMed] [Google Scholar]
  14. LITTLE J. R., BRECHER G., BRADLEY T. R., ROSE S. Determination of lymphocyte turnover by continuous infusion of H3 thymidine. Blood. 1962 Feb;19:236–242. [PubMed] [Google Scholar]
  15. MOSES M. J. APPLICATION OF AUTORADIOGRAPHY TO ELECTRON MICROSCOPY. J Histochem Cytochem. 1964 Feb;12:115–130. doi: 10.1177/12.2.115. [DOI] [PubMed] [Google Scholar]
  16. NOSSAL G. J., MAKELA O. Autoradiographic studies on the immune response.I. The kinetics of plasma cell proliferation. J Exp Med. 1962 Jan 1;115:209–230. doi: 10.1084/jem.115.1.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PRUSOFF W. H. Synthesis and biological activities of iododeoxyuridine, an analog of thymidine. Biochim Biophys Acta. 1959 Mar;32(1):295–296. doi: 10.1016/0006-3002(59)90597-9. [DOI] [PubMed] [Google Scholar]
  18. RIEKE W. O. The in vivo reutilization of lymphocytic and sarcoma DNA by cells growing in the peritoneal cavity. J Cell Biol. 1962 May;13:205–216. doi: 10.1083/jcb.13.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. ROBINSON S. H., BRECHER G. DELAYED INCORPORATION OF TRITIATED THYMIDINE INTO DNA. Science. 1963 Oct 18;142(3590):392–393. doi: 10.1126/science.142.3590.392. [DOI] [PubMed] [Google Scholar]
  20. ROBINSON S. H., BRECHER G., LOURIE I. S., HALEY J. E. LEUKOCYTE LABELING IN RATS DURING AND AFTER CONTINUOUS INFUSION OF TRITIATED THYMIDINE: IMPLICATIONS FOR LYMPHOCYTE LONGEVITY AND DNA REUTILIZATION. Blood. 1965 Sep;26:281–295. [PubMed] [Google Scholar]
  21. SCHOOLEY J. C. Autoradiographic observations of plasma cell formation. J Immunol. 1961 Mar;86:331–337. [PubMed] [Google Scholar]
  22. URSO P., MAKINODAN T. THE ROLES OF CELLULAR DIVISION AND MATURATION IN THE FORMATION OF PRECIPITATING ANTIBODY. J Immunol. 1963 Jun;90:897–907. [PubMed] [Google Scholar]
  23. WISSLER R. W., FITCH F. W., LA VIA M. F., GUNDERSON C. H. The cellular basis for antibody formation. J Cell Physiol Suppl. 1957 Dec;50(Suppl 1):265–301. doi: 10.1002/jcp.1030500417. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES