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. 1968 Oct 1;128(4):729–751. doi: 10.1084/jem.128.4.729

DISTRIBUTION OF ANTIBODY-FORMING CELLS OF DIFFERENT SPECIFICITIES IN THE LYMPH NODES AND SPLEENS OF GUINEA PIGS

Ira Green 1
PMCID: PMC2138538  PMID: 4877427

Abstract

The distribution of antibody-forming cells of different specificities in the lymph nodes and spleens of guinea pigs immunized with two separate antigens or with antigens bearing two determinants was studied. When animals were immunized with two soluble protein antigens or antigens in which the two antigenic determinants were on the same molecule, antibody-forming cells of different specificities were always randomly intermixed. However, when animals were immunized with two heat-aggregated particulate protein antigens and then boosted with soluble protein antigens, cells of different specificities were often seen to occur in groups. These results suggest that antibody-forming cells may not arise by the antigen-stimulated proliferation of precommitted antibody-forming cells, but rather antibody-forming cells arise by a transformation of uncommitted precursor cells as the result of their interactions with a locally produced material derived from the processing of antigen.

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Selected References

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  1. ASKONAS B. A., WHITE R. G. Sites of antibody production in the guinea-pig; the relation between in vitro synthesis of anti-ovalbumin and gamma-globulin and distribution of antibody-containing plasma cells. Br J Exp Pathol. 1956 Feb;37(1):61–74. [PMC free article] [PubMed] [Google Scholar]
  2. BENACERRAF B., LEVINE B. B. Immunological specificity of delayed and immediate hypersensitivity reactions. J Exp Med. 1962 May 1;115:1023–1036. doi: 10.1084/jem.115.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bernier G. M., Cebra J. J. Frequency distribution of alpha, gamma, kappa and lambda polypeptide chains in human lymphoid tissues. J Immunol. 1965 Aug;95(2):246–253. [PubMed] [Google Scholar]
  4. COONS A. H., LEDUC E. H., CONNOLLY J. M. Studies on antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med. 1955 Jul 1;102(1):49–60. doi: 10.1084/jem.102.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ehrich W. E., Harris T. N. THE FORMATION OF ANTIBODIES IN THE POPLITEAL LYMPH NODE IN RABBITS. J Exp Med. 1942 Oct 1;76(4):335–348. doi: 10.1084/jem.76.4.335. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. FARAH F. S., KERN M., EISEN H. N. The preparation and some properties of purified antibody specific for the 2,4-dinitrophenyl group. J Exp Med. 1960 Dec 1;112:1195–1210. doi: 10.1084/jem.112.6.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FISHMAN M., ADLER F. L. Antibody formation initiated in vitro. II. Antibody synthesis in x-irradiated recipients of diffusion chambers containing nucleic acid derived from macrophages incubated with antigen. J Exp Med. 1963 Apr 1;117:595–602. doi: 10.1084/jem.117.4.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GELL P. G., BENACERRAF B. Studies on hypersensitivity. II. Delayed hypersensitivity to denatured proteins in guinea pigs. Immunology. 1959 Jan;2(1):64–70. [PMC free article] [PubMed] [Google Scholar]
  9. Gallily R., Feldman M. The role of macrophages in the induction of antibody in x-irradiated animals. Immunology. 1967 Feb;12(2):197–206. [PMC free article] [PubMed] [Google Scholar]
  10. Green I., Paul W. E., Benacerraf B. Hapten carrier relationships in the DNP-PLL foreign albumin complex system: induction of tolerance and stimulation of cells in vitro. J Exp Med. 1968 Jan 1;127(1):43–53. doi: 10.1084/jem.127.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green I., Paul W. E., Benacerraf B. The behavior of hapten-poly-L-lysine conjugates as complete antigens in genetic responder and as haptens in nonresponder guinea pigs. J Exp Med. 1966 May 1;123(5):859–879. doi: 10.1084/jem.123.5.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Green I., Vasalli P., Benacerraf B. Cellular localization of anti-DNP-PLL and anticonveyor albumin antibodies in genetic nonresponder guinea pigs immunized with DNP-PLL albumin complexes. J Exp Med. 1967 Mar 1;125(3):527–536. doi: 10.1084/jem.125.3.527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Green I., Vassalli P., Nussenzweig V., Benacerraf B. Specificity of the antibodies produced by single cells following immunization with antigens bearing two types of antigenic determinants. J Exp Med. 1967 Mar 1;125(3):511–526. doi: 10.1084/jem.125.3.511. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hall J. G., Morris B., Moreno G. D., Bessis M. C. The ultrastructure and function of the cells in lymph following antigenic stimulation. J Exp Med. 1967 Jan 1;125(1):91–110. doi: 10.1084/jem.125.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. KANTOR F. S., OJEDA A., BENCARERRAF B. Studies on artifical antigens. I. Antigenicity of DNP-polylysine and DNP copolymer of lysine and glutamic acid in guinea pigs. J Exp Med. 1963 Jan 1;117:55–69. doi: 10.1084/jem.117.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Kabat E. A. Unique features of the variable regions of Bence Jones proteins and their possible relation to antibody complementarity. Proc Natl Acad Sci U S A. 1968 Feb;59(2):613–619. doi: 10.1073/pnas.59.2.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. LANGEVOORT H. L. The histophysiology of the antibody response. I. Histogenesis of the plasma cell reaction in rabbit spleen. Lab Invest. 1963 Jan;12:106–118. [PubMed] [Google Scholar]
  18. LEDUC E. H., COONS A. H., CONNOLLY J. M. Studies on antibody production. II. The primary and secondary responses in the popliteal lymph node of the rabbit. J Exp Med. 1955 Jul 1;102(1):61–72. doi: 10.1084/jem.102.1.61. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. LEVINE B. B., BENACERRAF B. GENETIC CONTROL IN GUINEA PIGS OF IMMUNE RESPONSE TO CONJUGATES OF HAPTENS AND POLY-L-LYSINE. Science. 1965 Jan 29;147(3657):517–518. doi: 10.1126/science.147.3657.517. [DOI] [PubMed] [Google Scholar]
  20. LEVINE B. B., OJEDA A., BENACERRAF B. STUDIES ON ARTIFICIAL ANTIGENS. III. THE GENETIC CONTROL OF THE IMMUNE RESPONSE TO HAPTEN-POLY-L-LYSINE CONJUGATES IN GUINEA PIGS. J Exp Med. 1963 Dec 1;118:953–957. doi: 10.1084/jem.118.6.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. MAKELA O., NOSSAL G. J. Study of antibody-producing capacity of single cells by bacterial adherence and immobilization. J Immunol. 1961 Oct;87:457–463. [PubMed] [Google Scholar]
  22. MCDEVITT H. O., PETERS J. H., POLLARD L. W., HARTER J. G., COONS A. H. PURIFICATION AND ANALYSIS OF FLUORESCEIN-LABELED ANTISERA BY COLUMN CHROMATOGRAPHY. J Immunol. 1963 Apr;90:634–642. [PubMed] [Google Scholar]
  23. McDevitt H. O., Askonas B. A., Humphrey J. H., Schechter I., Sela M. The localization of antigen in relation to specific antibody-producing cells. I. Use of a synthetic polypeptide [(T,G)-A--L] labelled with iodine-125. Immunology. 1966 Oct;11(4):337–351. [PMC free article] [PubMed] [Google Scholar]
  24. McFarland W., Heilman D. H., Moorhead J. F. Functional anatomy of the lymphocyte in immunological reactions in vitro. J Exp Med. 1966 Nov 1;124(5):851–858. doi: 10.1084/jem.124.5.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. McMaster P. D., Hudack S. S. THE FORMATION OF AGGLUTININS WITHIN LYMPH NODES. J Exp Med. 1935 May 31;61(6):783–805. doi: 10.1084/jem.61.6.783. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Mitchell G. F., Miller J. F. Immunological activity of thymus and thoracic-duct lymphocytes. Proc Natl Acad Sci U S A. 1968 Jan;59(1):296–303. doi: 10.1073/pnas.59.1.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. NOSSAL G. J., ADA G. L., AUSTIN C. M. ANTIGENS IN IMMUNITY. IX. THE ANTIGEN CONTENT OF SINGLE ANTIBODY-FORMING CELLS. J Exp Med. 1965 Jun 1;121:945–954. doi: 10.1084/jem.121.6.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Nakano M., Braun W. Fluctuation tests with antibody-forming spleen cell populations. Science. 1966 Jan 21;151(3708):338–340. doi: 10.1126/science.151.3708.338. [DOI] [PubMed] [Google Scholar]
  29. Paul W. E., Siskind G. W., Benacerraf B., Ovary Z. Secondary antibody responses in haptenic systems: cell population selection by antigen. J Immunol. 1967 Oct;99(4):760–770. [PubMed] [Google Scholar]
  30. Pernis B., Chiappino G., Kelus A. S., Gell P. G. Cellular localization of immunoglobulins with different allotypic specificities in rabbit lymphoid tissues. J Exp Med. 1965 Nov 1;122(5):853–876. doi: 10.1084/jem.122.5.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schechter I. Antigenic competition between polypeptidyl determinants in normal and tolerant rabbits. J Exp Med. 1968 Feb 1;127(2):237–250. doi: 10.1084/jem.127.2.237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Smithies O. Antibody Induction and Tolerance. Science. 1965 Jul 9;149(3680):151–156. doi: 10.1126/science.149.3680.151. [DOI] [PubMed] [Google Scholar]
  33. Steiner L. A., Eisen H. N. The relative affinity of antibodies synthesized in the secondary response. J Exp Med. 1967 Dec 1;126(6):1185–1205. doi: 10.1084/jem.126.6.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Szilard L. THE MOLECULAR BASIS OF ANTIBODY FORMATION. Proc Natl Acad Sci U S A. 1960 Mar;46(3):293–302. doi: 10.1073/pnas.46.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Trentin J., Wolf N., Cheng V., Fahlberg W., Weiss D., Bonhag R. Antibody production by mice repopulated with limited numbers of clones of lymphoid cell precursors. J Immunol. 1967 Jun;98(6):1326–1337. [PubMed] [Google Scholar]
  36. WHITE R. G. Antibody production by single cells. Nature. 1958 Nov 15;182(4646):1383–1384. doi: 10.1038/1821383a0. [DOI] [PubMed] [Google Scholar]

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