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. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801

CELL TO CELL INTERACTION IN THE IMMUNE RESPONSE

I. HEMOLYSIN-FORMING CELLS IN NEONATALLY THYMECTOMIZED MICE RECONSTITUTED WITH THYMUS OR THORACIC DUCT LYMPHOCYTES

J F A P Miller 1, G F Mitchell 1
PMCID: PMC2138540  PMID: 5691985

Abstract

An injection of viable thymus or thoracic duct lymphocytes was absolutely essential to enable a normal or near-normal 19S liemolysin-forming cell response in the spleens of neonatally thymectomized mice challenged with sheep erythrocytes. Syngeneic thymus lymphocytes were as effective as thoracic duct lymphocytes in this system and allogeneic or semiallogeneic cells could also reconstitute their hosts. No significant elevation of the response was achieved by giving either bone marrow cells, irradiated thymus or thoracic duct cells, thymus extracts or yeast. Spleen cells from reconstituted mice were exposed to anti-H2 sera directed against either the donor of the thymus or thoracic duct cells, or against the neonatally thymectomized host. Only isoantisera directed against the host could significantly reduce the number of hemolysin-forming cells present in the spleen cell suspensions. It is concluded that these antibody-forming cells are derived, not from the inoculated thymus or thoracic duct lymphocytes, but from the host. Thoracic duct cells from donors specifically immunologically tolerant of sheep erythrocytes had a markedly reduced restorative capacity in neonatally thymectomized recipients challenged with sheep erythrocytes. These results have suggested that there are cell types, in thymus or thoracic duct lymph, with capacities to react specifically with antigen and to induce the differentiation, to antibody-forming cells, of hemolysin-forming cell precursors derived from a separate cell line present in the neonatally thymectomized hosts.

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Selected References

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  1. BRAUN W., NAKANO M. INFLUENCE OF OLIGODEOXYRIBONUCLEOTIDES ON EARLY EVENTS IN ANTIBODY FORMATION. Proc Soc Exp Biol Med. 1965 Jul;119:701–707. doi: 10.3181/00379727-119-30276. [DOI] [PubMed] [Google Scholar]
  2. Celada F., Klein G. Autonomy of H-2 genes in individual immunocytes. Nature. 1967 Sep 9;215(5106):1136–1139. doi: 10.1038/2151136a0. [DOI] [PubMed] [Google Scholar]
  3. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  4. Dietrich F. M., Dukor P. The immune response to heterologous red cells in mice. 3. Cyclophosphamide-induced tolerance to multispecies rd cells. Pathol Microbiol (Basel) 1967;30(6):909–917. doi: 10.1159/000161757. [DOI] [PubMed] [Google Scholar]
  5. EAST J., PARROTT D. M. PREVENTION OF WASTING IN MICE THYMECTOMIZED AT BIRTH AND THEIR SUBSEQUENT REJECTION OF ALLOGENEIC LEUKEMIC CELLS. J Natl Cancer Inst. 1964 Oct;33:673–690. [PubMed] [Google Scholar]
  6. FRIEDMAN H. DISTRIBUTION OF ANTIBODY PLAQUE FORMING CELLS IN VARIOUS TISSUES OF SEVERAL STRAINS OF MICE INJECTED WITH SHEEP ERYTHROCYTES. Proc Soc Exp Biol Med. 1964 Nov;117:526–530. doi: 10.3181/00379727-117-29628. [DOI] [PubMed] [Google Scholar]
  7. Goldschneider I., McGregor D. D. Migration of lymphocytes and thymocytes in the rat. I. The route of migration from blood to spleen and lymph nodes. J Exp Med. 1968 Jan 1;127(1):155–168. doi: 10.1084/jem.127.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gowans J. L., Uhr J. W. The carriage of immunological memory by small lymphocytes in the rat. J Exp Med. 1966 Nov 1;124(5):1017–1030. doi: 10.1084/jem.124.5.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. HILGARD H. R., YUNIS E. J., SJODIN K., MARTINEZ C., GOOD R. A. REVERSAL OF WASTING IN THYMECTOMIZED MICE BY THE INJECTION OF SYNGENEIC SPLEEN OR THYMUS CELL SUSPENSIONS. Nature. 1964 May 16;202:668–670. doi: 10.1038/202668a0. [DOI] [PubMed] [Google Scholar]
  10. Isaković K., Waksman B. H., Wennersten C. Immunologic reactivity in neonatally thymectomized rats receiving competent lymphoid cells during immunization. J Immunol. 1965 Oct;95(4):602–613. [PubMed] [Google Scholar]
  11. JAROSLOW B. N., TALIAFERRO W. H. The restoration of hemolysin-forming capacity in x-irradiated rabbits by tissue and yeast preparations. J Infect Dis. 1956 Jan-Feb;98(1):75–81. doi: 10.1093/infdis/98.1.75. [DOI] [PubMed] [Google Scholar]
  12. METCALF D. Further studies on the plasma lymphocytosis stimulating factor in chronic lymphatic leukaemia and some other disease states. Br J Cancer. 1956 Sep;10(3):431–441. doi: 10.1038/bjc.1956.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. MILLER J. F., HOWARD J. G. SOME SIMILARITIES BETWEEN THE NEONATAL THYMECTOMY SYNDROME AND GRAFT-VERSUS-HOST DISEASE. J Reticuloendothel Soc. 1964 Dec;1:369–392. [PubMed] [Google Scholar]
  14. MILLER J. F. Studies on mouse leukaemia. The role of the thymus in leukaemogenesis by cell-free leukaemic filtrates. Br J Cancer. 1960 Mar;14:93–98. doi: 10.1038/bjc.1960.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MILLER J. F. THE THYMUS AND THE DEVELOPMENT OF IMMUNOLOGIC RESPONSIVENESS. Science. 1964 Jun 26;144(3626):1544–1551. doi: 10.1126/science.144.3626.1544. [DOI] [PubMed] [Google Scholar]
  16. McGregor D. D., McCullagh P. J., Gowans J. L. The role of lymphocytes in antibody formation. I. Restoration of the haemolysin response in x-irradiated rats with lymphocytes from normal and immunologically tolerant donors. Proc R Soc Lond B Biol Sci. 1967 Sep 12;168(1012):229–243. doi: 10.1098/rspb.1967.0063. [DOI] [PubMed] [Google Scholar]
  17. Miller J. F., Mitchell G. F. The thymus and the precursors of antigen reactive cells. Nature. 1967 Nov 18;216(5116):659–663. doi: 10.1038/216659a0. [DOI] [PubMed] [Google Scholar]
  18. Miller J. F., Mitchell G. F., Weiss N. S. Cellular basis of the immunological defects in thymectomized mice. Nature. 1967 Jun 3;214(5092):992–997. doi: 10.1038/214992a0. [DOI] [PubMed] [Google Scholar]
  19. Miller J. F., Osoba D. Current concepts of the immunological function of the thymus. Physiol Rev. 1967 Jul;47(3):437–520. doi: 10.1152/physrev.1967.47.3.437. [DOI] [PubMed] [Google Scholar]
  20. Miller J. F. The thymus. Yesterday, today, and tomorrow. Lancet. 1967 Dec 16;2(7529):1299–1302. doi: 10.1016/s0140-6736(67)90407-2. [DOI] [PubMed] [Google Scholar]
  21. Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mitchell G. F., Miller J. F. Immunological activity of thymus and thoracic-duct lymphocytes. Proc Natl Acad Sci U S A. 1968 Jan;59(1):296–303. doi: 10.1073/pnas.59.1.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. NOSSAL G. J. STUDIES ON THE RATE OF SEEDING OF LYMPHOCYTES FROM THE INTACT GUINEA PIG THYMUS. Ann N Y Acad Sci. 1964 Nov 30;120:171–181. doi: 10.1111/j.1749-6632.1964.tb34715.x. [DOI] [PubMed] [Google Scholar]
  24. OSOBA D., MILLER J. F. THE LYMPHOD TISSUES AND IMMUNE RESPONSES OF NEONATALLY THYMECTOMIZED MICE BEARING THYMUS TISSUE IN MILLIPORE DIFFUSION CHAMBERS. J Exp Med. 1964 Jan 1;119:177–194. doi: 10.1084/jem.119.1.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Parrott D. V., De Sousa M. A., East J. Thymus-dependent areas in the lymphoid organs of neonatally thymectomized mice. J Exp Med. 1966 Jan 1;123(1):191–204. doi: 10.1084/jem.123.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Schooley J. C., Kelly L. S., Dobson E. L., Finney C. R., Havens V. W., Cantor L. N. Reticuloendothelial activity in neonatally thymectomized mice and irradiated mice thymectomized in adult life. J Reticuloendothel Soc. 1965 Dec;2(5):396–405. [PubMed] [Google Scholar]
  27. TAYLOR R. B. IMMUNOLOGICAL COMPETENCE OF THYMUS CELLS AFTER TRANSFER TO THYMECTOMIZED RECIPIENTS. Nature. 1963 Aug 31;199:873–874. doi: 10.1038/199873a0. [DOI] [PubMed] [Google Scholar]
  28. TRAININ N., LAW L. W., LEVEY R. H. PATTERNS OF RECONSTITUTION OF NEONATALLY THYMECTOMIZED MICE BY INJECTIONS OF ISOLATED LYMPHOPOIETIC AND HEMATOPOIETIC CELLS. Proc Soc Exp Biol Med. 1965 Jan;118:79–85. doi: 10.3181/00379727-118-29762. [DOI] [PubMed] [Google Scholar]
  29. Weissman I. L. Thymus cell migration. J Exp Med. 1967 Aug 1;126(2):291–304. doi: 10.1084/jem.126.2.291. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. YUNIS E. J., HILGARD H., SJODIN K., MARTINEZ C., GOOD R. A. IMMUNOLOGICAL RECONSTITUTION OF THYMECTOMIZED MICE BY INJECTIONS OF ISOLATED THYMOCYTES. Nature. 1964 Feb 22;201:784–786. doi: 10.1038/201784a0. [DOI] [PubMed] [Google Scholar]

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