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. 1968 Oct 1;128(4):855–874. doi: 10.1084/jem.128.4.855

CELL TO CELL INTERACTION IN THE IMMUNE RESPONSE

IV. SITE OF ACTION OF ANTILYMPHOCYTE GLOBULIN

W J Martin 1, J F A P Miller 1
PMCID: PMC2138548  PMID: 5691988

Abstract

In this series of papers it has been shown that the immune response of mice to sheep erythrocytes requires the participation of two classes of lymphoid cells. Thymus-derived cells initially react with antigen and then interact with another class of cells, the antibody-forming cell precursors, to cause their differentiation to antibody-forming cells. Antilymphocyte globulin depressed the ability of mice to respond to sheep erythrocytes. This effect was more marked when the antigen was injected intraperitoneally than intravenously, and occurred only when the antilymphocyte globulin was given before or simultaneously with antigen. Injection of thymus cells restored to near normal the ability to respond to an intravenous injection of sheep erythrocytes. Spleen cells from antilymphocyte globulin-treated mice gave a weak adoptive immune response in irradiated recipients. The addition of thymus cells however enabled a response similar to that given by normal spleen cells. When thymectomized irradiated recipients were used, normal spleen cells continued to give a higher response to a challenge of sheep erythrocytes at 2 and 4 wk postirradiation than did spleen cells from ALG-treated donors. This result is more consistent with the notion that thymus-derived target cells are eliminated, rather than temporarily inactivated, by antilymphocyte globulin. These findings suggest that, in vivo, antilymphocyte globulin acts selectively on the thymus-derived antigen-reactive cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BILLINGHAM R. E., BRENT L., MEDAWAR P. B. Quantitative studies on tissue transplantation immunity. II. The origin, strength and duration of actively and adoptively acquired immunity. Proc R Soc Lond B Biol Sci. 1954 Dec 15;143(910):58–80. doi: 10.1098/rspb.1954.0054. [DOI] [PubMed] [Google Scholar]
  2. Bainbridge D. R., Brent L., Gowland G. Distribution of allogeneic 51Cr-labelled lymph node cells in mice. Transplantation. 1966 Mar;4(2):138–153. doi: 10.1097/00007890-196603000-00003. [DOI] [PubMed] [Google Scholar]
  3. Berenbaum M. C. Time-dependent immunosuppressive effects of anti-thymocyte serum. Nature. 1967 Sep 30;215(5109):1481–1482. doi: 10.1038/2151481a0. [DOI] [PubMed] [Google Scholar]
  4. CONVERSE J. M., BALLANTYNE D. L., Jr, WOISKY J. The vascularization of skin homografts and transplantation immunity. Ann N Y Acad Sci. 1958 Oct 7;73(3):693–697. doi: 10.1111/j.1749-6632.1959.tb40846.x. [DOI] [PubMed] [Google Scholar]
  5. CROSS A. M., LEUCHARS E., MILLER J. F. STUDIES ON THE RECOVERY OF THE IMMUNE RESPONSE IN IRRADIATED MICE THYMECTOMIZED IN ADULT LIFE. J Exp Med. 1964 May 1;119:837–850. doi: 10.1084/jem.119.5.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  7. Goldschneider I., McGregor D. D. Migration of lymphocytes and thymocytes in the rat. I. The route of migration from blood to spleen and lymph nodes. J Exp Med. 1968 Jan 1;127(1):155–168. doi: 10.1084/jem.127.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guttmann R. D., Carpenter C. B., Lindquist R. R., Merrill J. P. Renal transplantation in the inbred rat. 3. A study of heterologous anti-thymocyte sera. J Exp Med. 1967 Dec 1;126(6):1099–1126. doi: 10.1084/jem.126.6.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Levey R. H., Medawar P. B. Further experiments on the action of antilymphocytic antiserum. Proc Natl Acad Sci U S A. 1967 Aug;58(2):470–477. doi: 10.1073/pnas.58.2.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Levey R. H., Medawar P. B. Nature and mode of action of antilymphocytic antiserum. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1130–1137. doi: 10.1073/pnas.56.4.1130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MILLER J. F. Studies on mouse leukaemia. The role of the thymus in leukaemogenesis by cell-free leukaemic filtrates. Br J Cancer. 1960 Mar;14:93–98. doi: 10.1038/bjc.1960.11. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Martin W. J., Miller J. F. Site of action of antilymphocyte globulin. Lancet. 1967 Dec 16;2(7529):1285–1287. doi: 10.1016/s0140-6736(67)90395-9. [DOI] [PubMed] [Google Scholar]
  13. McKhann C. F., Berrian J. H. Transplantation Immunity: Some Properties of Induction and Expression. Ann Surg. 1959 Dec;150(6):1025–1031. doi: 10.1097/00000658-195912000-00009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miller J. F., Mitchell G. F., Weiss N. S. Cellular basis of the immunological defects in thymectomized mice. Nature. 1967 Jun 3;214(5092):992–997. doi: 10.1038/214992a0. [DOI] [PubMed] [Google Scholar]
  16. Miller J. F., Osoba D. Current concepts of the immunological function of the thymus. Physiol Rev. 1967 Jul;47(3):437–520. doi: 10.1152/physrev.1967.47.3.437. [DOI] [PubMed] [Google Scholar]
  17. Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Monaco A. P., Wood M. L., Gray J. G., Russell P. S. Studies on heterologous anti-lymphocyte serum in mice. II. Effect on the immune response. J Immunol. 1966 Feb;96(2):229–238. [PubMed] [Google Scholar]
  19. Monaco A. P., Wood M. L., Russell P. S. Adult Thymectomy: Effect on Recovery from Immunologic Depression in Mice. Science. 1965 Jul 23;149(3682):432–435. doi: 10.1126/science.149.3682.432. [DOI] [PubMed] [Google Scholar]
  20. Parrott D. V., De Sousa M. A., East J. Thymus-dependent areas in the lymphoid organs of neonatally thymectomized mice. J Exp Med. 1966 Jan 1;123(1):191–204. doi: 10.1084/jem.123.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pinnas J. L., Fitch F. W. Immunologic competence of thymectomized rats to several soluble and particulate antigens. Int Arch Allergy Appl Immunol. 1966;30(3):217–230. doi: 10.1159/000229807. [DOI] [PubMed] [Google Scholar]
  22. SCOTHORNE R. J. Studies on the response of the regional lymph node to skin homografts. Ann N Y Acad Sci. 1957 Mar 22;64(5):1028-38; discussion, 1038-9. doi: 10.1111/j.1749-6632.1957.tb52494.x. [DOI] [PubMed] [Google Scholar]
  23. STROBER S., GOWANS J. L. THE ROLE OF LYMPHOCYTES IN THE SENSITIZATION OF RATS TO RENAL HOMOGRAFTS. J Exp Med. 1965 Aug 1;122:347–360. doi: 10.1084/jem.122.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Turk J. L., Willoughby D. A. Central and peripheral effects of anti-lymphocyte sera. Lancet. 1967 Feb 4;1(7484):249–251. doi: 10.1016/s0140-6736(67)91307-4. [DOI] [PubMed] [Google Scholar]
  25. Woodruff M. F. Purification of antilymphocytic antibody. Nature. 1968 Mar 2;217(5131):821–824. doi: 10.1038/217821a0. [DOI] [PubMed] [Google Scholar]

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