Abstract
Isolated segments of human colon and to a lesser extent ileum were capable of synthesizing hemolytically active C'1. This conclusion was based on the following evidence: After elimination of C'1 from tissue with EDTA, we found that segments of the intestinal tract in short-term organ culture showed a 50–1000-fold increase in C'1 activity. The rate of production of C'1 in human intestine was highly temperature dependent; C'1 production was reversibly inhibited by puromycin and actinomycin D. Furthermore, 14C-labeled amino acids were incorporated into molecules which behaved like C'1. No significant C'1hu synthesis was observed in isolated segments of jejunum, stomach, liver, kidney, lung, spleen, lymph node, and thymus.
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- BORSOS T., RAPP H. J. CHROMATOGRAPHIC SEPARATION OF THE FIRST COMPONENT OF COMPLEMENT AND ITS ASSAY ON A MOLECULAR BASIS. J Immunol. 1963 Dec;91:851–858. [PubMed] [Google Scholar]
- Borsos T., Rapp H. J. Hemolysin titration based on fixation of the activated first component of complement: evidence that one molecule of hemolysin suffices to sensitize an erythrocyte. J Immunol. 1965 Sep;95(3):559–566. [PubMed] [Google Scholar]
- Borsos T., Rapp H. J. Immune hemolysis: a simplified method for the preparation of EAC'4 with guinea pig or with human complement. J Immunol. 1967 Aug;99(2):263–268. [PubMed] [Google Scholar]
- Colten H. R., Borsos T., Rapp H. J. In vitro synthesis of the first component of complement by guinea pig small intestine. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1158–1163. doi: 10.1073/pnas.56.4.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Colten H. R., Gordon J. M., Rapp H. J., Borsos T. Synthesis of the first component of guinea pig complement by columnar epithelial cells of the small intestine. J Immunol. 1968 Apr;100(4):788–792. [PubMed] [Google Scholar]
- Gewurz H., Pickering R. J., Muschel L. H., Mergenhagen S. E., Good R. A. Complement-dependent biological functions in complement deficiency in man. Lancet. 1966 Aug 13;2(7459):356–360. doi: 10.1016/s0140-6736(66)92658-4. [DOI] [PubMed] [Google Scholar]
- JONSEN J., KASS E. Agammaglobulinemia; report of titrations of complement components in a case. Acta Pathol Microbiol Scand. 1957;41(5):393–396. [PubMed] [Google Scholar]
- Jacobs J. C., de Capoa A., McGilvray E., Morse J. H., Schullinger J. N., Blanc W. A., Heird W. C., Miller O. J., Rossen R. D., Walzer R. A. Complement deficiency and chromosomalbreaks in a case of Swiss-type agammaglobulinaemia. Lancet. 1968 Mar 9;1(7541):499–503. doi: 10.1016/s0140-6736(68)91467-0. [DOI] [PubMed] [Google Scholar]
- MULLER-EBERHARD H. J., KUNKEL H. G. Isolation of a thermolabile serum protein which precipitates gamma-globulin aggregates and participates in immune hemolysis. Proc Soc Exp Biol Med. 1961 Feb;106:291–295. doi: 10.3181/00379727-106-26313. [DOI] [PubMed] [Google Scholar]
- O'Connell E. J., Enriquez P., Linman J. W., Gleich G. J., McDuffie F. C. Absence of activity of first component of complement in man: association with thymic alymphoplasia and defective inflammatory response. J Lab Clin Med. 1967 Nov;70(5):715–724. [PubMed] [Google Scholar]
- RAPP H. J., BORSOS T. EFFECTS OF LOW IONIC STRENGTH ON IMMUNE HEMOLYSIS. J Immunol. 1963 Dec;91:826–832. [PubMed] [Google Scholar]
- Rapp H. J., Borsos T. Forssman antigen and antibody: preparation of water soluble antigen and measurement of antibody concentration by precipitin analysis, by C'1a fixation and by hemolytic activity. J Immunol. 1966 Jun;96(6):913–919. [PubMed] [Google Scholar]
- Stecher V. J., Morse J. H., Thorbecke G. J. Sites of production of primate serum proteins associated with complement system. Proc Soc Exp Biol Med. 1967 Feb;124(2):433–438. doi: 10.3181/00379727-124-31758. [DOI] [PubMed] [Google Scholar]