Abstract
Two mechanisms have been described whereby rabbit platelets in vitro may be induced to release their contained histamine by the reaction of antigen and antibody. Both processes require the participation of the complement system. In the first, the adherence of platelets to particulate antigens such as zymosan or erythrocytes which have fixed complement through the third component was followed by histamine release. Plasma lacking C6 activity was fully active in this system. In the second mechanism, the reaction of soluble antigen with antibody in the presence of plasma also caused release of histamine from platelets and platelet clumping was observed. The release process, which appeared to follow the adherence of platelets to the immune complex, required the action of C6 and perhaps the later-acting components. No evidence could be obtained that a soluble factor was produced which caused the release. Instead, an inhibitor of the release process was detected after incubation of antigen, antibody, and plasma. Antibody preparations capable of giving complement-dependent PCA reactions in rabbits were also shown to induce the release of histamine from platelets in vitro.
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Selected References
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- BARBARO J. F. The release of histamine from rabbit platelets by means of antigen-antibody precipitates. I. The participation of the immune complex in histamine release. J Immunol. 1961 Apr;86:369–376. [PubMed] [Google Scholar]
- BARBARO J. F. The release of histamine from rabbit platelets by means of antigen-antibody precipitates. II. The role of plasma in the release of histamine. J Immunol. 1961 Apr;86:377–381. [PubMed] [Google Scholar]
- Cooper N. R., Becker E. L. Complement associated peptidase activity of guinea pig serum. I. Role of complement components. J Immunol. 1967 Jan;98(1):119–131. [PubMed] [Google Scholar]
- GOCKE D. J. IN VITRO DAMAGE OF RABBIT PLATELETS BY AN UNRELATED ANTIGEN-ANTIBODY REACTION. II. STUDIES OF THE PLASMA REQUIREMENT. J Immunol. 1965 Feb;94:247–252. [PubMed] [Google Scholar]
- GOCKE D. J., OSLER A. G. IN VITRO DAMAGE OF RABBIT PLATELETS BY AN UNRELATED ANTIGEN-ANTIBODY REACTION. I. GENERAL CHARACTERISTICS OF THE REACTION. J Immunol. 1965 Feb;94:236–246. [PubMed] [Google Scholar]
- HUMPHREY J. H., JAQUES R. The release of histamine and 5-hydroxytryptamine (serotonin) from platelets by antigen-antibody reactions (in vitro). J Physiol. 1955 Apr 28;128(1):9–27. doi: 10.1113/jphysiol.1955.sp005289. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henson P. M., Cochrane C. G. Immunological induction of increased vascular permeability. I. A rabbit passive cutaneous anaphylactic reaction requiring complement, platelets, and neutrophils. J Exp Med. 1969 Jan 1;129(1):153–165. doi: 10.1084/jem.129.1.153. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Henson P. M. The adherence of leucocytes and platelets induced by fixed IgG antibody or complement. Immunology. 1969 Jan;16(1):107–121. [PMC free article] [PubMed] [Google Scholar]
- Kniker W. T., Cochrane C. G. The localization of circulating immune complexes in experimental serum sickness. The role of vasoactive amines and hydrodynamic forces. J Exp Med. 1968 Jan 1;127(1):119–136. doi: 10.1084/jem.127.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LINSCOTT W. D., COCHRANE C. G. GUINEA PIG BETA-1C-GLOBULIN: ITS RELATIONSHIP TO THE THIRD COMPONENT OF COMPLEMENT AND ITS ALTERATION FOLLOWING INTERACTION WITH IMMUNE COMPLEXES. J Immunol. 1964 Dec;93:972–984. [PubMed] [Google Scholar]
- Movat H. Z., Mustard J. F., Taichman N. S., Uriuhara T. Platelet aggregation and release of ADP, serotonin and histamine associated with phagocytosis of antigen-antibody complexes. Proc Soc Exp Biol Med. 1965 Oct;120(1):232–237. doi: 10.3181/00379727-120-30496. [DOI] [PubMed] [Google Scholar]
- NILSSON U. R., MUELLER-EBERHARD H. J. ISOLATION OF BETA IF-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT. J Exp Med. 1965 Aug 1;122:277–298. doi: 10.1084/jem.122.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spielvogel A. R. An ultrastructural study of the mechanisms of platelet-endotoxin interaction. J Exp Med. 1967 Aug 1;126(2):235–250. doi: 10.1084/jem.126.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward P. A., Becker E. L. Mechanisms of the inhibition of chemotaxis by phosphonate esters. J Exp Med. 1967 Jun 1;125(6):1001–1020. doi: 10.1084/jem.125.6.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]