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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1969 Jan 31;129(2):411–429. doi: 10.1084/jem.129.2.411

THE HUMAN SECRETORY IMMUNOGLOBULIN SYSTEM: IMMUNOHISTOLOGICAL LOCALIZATION OF γA, SECRETORY "PIECE," AND LACTOFERRIN IN NORMAL HUMAN TISSUES

D R Tourville 1, R H Adler 1, J Bienenstock 1, T B Tomasi Jr 1
PMCID: PMC2138606  PMID: 4178353

Abstract

The immunohistological localization of γA, secretory "piece" (SP), and lactoferrin (LF) in the mucosae of a variety of normal human tissues was investigated using specific fluoresceinated antisera. γA staining was localized in the apical portion of the mucosal epithelium, intercellular spaces, basement membrane area, and plasma cells of the interstitium or lamina propria of a number of normal human tissues. SP was ubiquitous in the mucosal epithelium of all tissues studied which included parotid and submaxillary glands, bronchi, pancreas, GI tract, sweat glands, kidney, and gall bladder. In addition, SP staining was localized in the intercellular spaces and on the surface of the epithelial cells lining the lumen of the secretory glands. No SP staining was observed in the plasma cells of the interstitium or lamina propria surrounding the secretory glands in these tissues, and no SP staining was observed in sections of normal spleen or lymph node tissue. SP staining was observed in the sweat glands, pancreas, and kidney in the absence of γA staining. LF was much less ubiquitous in the epithelial cells of the various tissues studied and appeared to be restricted primarily to the acinar epithelium of the bronchial mucosae, parotid, and submaxillary salivary glands, and was also found in renal tubular cells. A hypothetical model for the transport of γA and SP across mucosal membrane epithelium is presented.

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Selected References

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  1. Brandtzaeg P., Fjellanger I., Gjeruldsen S. T. Immunoglobulin M: local synthesis and selective secretion in patients with immunoglobulin A deficiency. Science. 1968 May 17;160(3829):789–791. doi: 10.1126/science.160.3829.789. [DOI] [PubMed] [Google Scholar]
  2. Brandtzaeg P., Fjellanger I., Gjeruldsen S. T. Localization of immunoglobulins in human nasal mucosa. Immunochemistry. 1967 Jan;4(1):57–60. doi: 10.1016/0019-2791(67)90197-8. [DOI] [PubMed] [Google Scholar]
  3. CRABBE P. A., CARBONARA A. O., HEREMANS J. F. THE NORMAL HUMAN INTESTINAL MUCOSA AS A MAJOR SOURCE OF PLASMA CELLS CONTAINING GAMMA-A-IMMUNOGLOBULIN. Lab Invest. 1965 Mar;14:235–248. [PubMed] [Google Scholar]
  4. Crabbé P. A., Heremans J. F. The distribution of immunoglobulin-containing cells along the human gastrointestinal tract. Gastroenterology. 1966 Sep;51(3):305–316. [PubMed] [Google Scholar]
  5. Crandall R. B., Cebra J. J., Crandall C. A. The relative proportions of IgG-, IgAand IgM-containing cells in rabbit tissues during experimental trichinosis. Immunology. 1967 Feb;12(2):147–158. [PMC free article] [PubMed] [Google Scholar]
  6. DIXON F. J., WEIGLE W. O., VAZQUEZ J. J. Metabolism and mammary secretion of serum proteins in the cow. Lab Invest. 1961 Mar-Apr;10:216–237. [PubMed] [Google Scholar]
  7. Eidelman S., Davis S. D. Immunoglobulin content of intestinal mucosal plasma-cells in ataxia telangiectasia. Lancet. 1968 Apr 27;1(7548):884–886. doi: 10.1016/s0140-6736(68)90238-9. [DOI] [PubMed] [Google Scholar]
  8. FARQUHAR M. G., PALADE G. E. Junctional complexes in various epithelia. J Cell Biol. 1963 May;17:375–412. doi: 10.1083/jcb.17.2.375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gelzayd E. A., Kraft S. C., Fitch F. W. Immunoglobulin A: localization in rectal mucosal epithelial cells. Science. 1967 Aug 25;157(3791):930–931. doi: 10.1126/science.157.3791.930. [DOI] [PubMed] [Google Scholar]
  10. Gelzayd E. A., Kraft S. C., Kirsner J. B. Distribution of immunoglobulins in human rectal mucosa. I. Normal control subjects. Gastroenterology. 1968 Mar;54(3):334–340. [PubMed] [Google Scholar]
  11. Hugon J. S., Borgers M. Absorption of horseradish peroxidase by the mucosal cells of the duodenum of mouse. I. The fasting animal. J Histochem Cytochem. 1968 Apr;16(4):229–236. doi: 10.1177/16.4.229. [DOI] [PubMed] [Google Scholar]
  12. Jeffries G. H., Sleisenger M. H. Studies of parietal cell antibody in pernicious anemia. J Clin Invest. 1965 Dec;44(12):2021–2028. doi: 10.1172/JCI105309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McFarlin D. E., Strober W., Wochner R. D., Waldmann T. A. Immunoglobulin A production in ataxia telangiectasia. Science. 1965 Nov 26;150(3700):1175–1177. doi: 10.1126/science.150.3700.1175. [DOI] [PubMed] [Google Scholar]
  14. Paronetto F., Koffler D. Immunofluorescent localization of immunoglobulins, complement, and fibrinogen in human diseases. I. Systemic lupus erythematosus. J Clin Invest. 1965 Oct;44(10):1657–1664. doi: 10.1172/JCI105272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. RUBIN W., FAUCI A. S., MARVIN S. F., SLEISENGER M. H., JEFRIES G. H. IMMUNOFLUORESCENT STUDIES IN ADULT CELIAC DISEASE. J Clin Invest. 1965 Mar;44:475–485. doi: 10.1172/JCI105161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rossen R. D., Morgan C., Hsu K. C., Butler W. T., Rose H. M. Localization of 11 S external secretory IgA by immunofluorescence in tissues lining the oral and respiratory passages in man. J Immunol. 1968 Apr;100(4):706–717. [PubMed] [Google Scholar]
  17. South M. A., Cooper M. D., Wollheim F. A., Hong R., Good R. A. The IgA system. I. Studies of the transport and immunochemistry of IgA in the saliva. J Exp Med. 1966 Apr 1;123(4):615–627. doi: 10.1084/jem.123.4.615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. TOMASI T. B., Jr, TAN E. M., SOLOMON A., PRENDERGAST R. A. CHARACTERISTICS OF AN IMMUNE SYSTEM COMMON TO CERTAIN EXTERNAL SECRETIONS. J Exp Med. 1965 Jan 1;121:101–124. doi: 10.1084/jem.121.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tomasi T. B., Jr, Bienenstock J. Secretory immunoglobulins. Adv Immunol. 1968;9:1–96. doi: 10.1016/s0065-2776(08)60441-1. [DOI] [PubMed] [Google Scholar]

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