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. 1969 Feb 28;129(3):523–550. doi: 10.1084/jem.129.3.523

THE OCCURRENCE AND PROPERTIES OF LEUKOCYTOSIS AND LYMPHOCYTOSIS-STIMULATING MATERIAL IN THE SUPERNATANT FLUIDS OF BORDETELLA PERTUSSIS CULTURES

Stephen I Morse 1, Karen K Bray 1
PMCID: PMC2138620  PMID: 4304138

Abstract

1. Leukocytosis- and lymphocytosis-stimulating activity was present in fluid cultures of B. pertussis. The activity was found primarily in the culture supernatant fluid. 2. The sequential changes in the leukocyte response were similar to those previously observed following injection of intact bacteria into mice. 3. Activity was destroyed by heat and was diminished, but not abolished, by prolonged treatment with proteolytic enzymes. 4. A water-insoluble fraction of the culture supernatant fluid was isolated which contained virtually all of the activity. The specific activity was more than 100-fold greater than that of the intact bacteria, and injection of microgram quantities produced a response. 5. The distribution of histamine-sensitizing factor followed that of leukocytosis-stimulating activity. In contrast, mouse protective antigen was localized to the bacterial pellet.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANERJEA A., MUNOZ J. Antigens of Bordetella pertussis. II. Purification of heat-labile toxin. J Bacteriol. 1962 Aug;84:269–274. doi: 10.1128/jb.84.2.269-274.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Greenbaum A. L., Clark J. B., McLean P. The activities of nicotinamide mononucleotide adenylyltransferase and of nicotinamide-adenine dinucleotide kinase in the livers of rats subjected to different hormonal treatments. Biochem J. 1965 Aug;96(2):507–516. doi: 10.1042/bj0960507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. KAGEYAMA M. STUDIES OF A PYOCIN. I. PHYSICAL AND CHEMICAL PROPERTIES. J Biochem. 1964 Jan;55:49–53. doi: 10.1093/oxfordjournals.jbchem.a127839. [DOI] [PubMed] [Google Scholar]
  4. KIND L. S. The altered reactivity of mice after inoculation with Bordetella pertussis vaccine. Bacteriol Rev. 1958 Sep;22(3):173–182. doi: 10.1128/br.22.3.173-182.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KUWAJIMA Y., MASUI M., ASANO A., MATSUI T. A soluble antigen of phase 1 Hemophilus pertussis. I. Mouse protection test of filtrate or supernatant of cultures in a medium without soluble starch or carbon particles. J Immunol. 1956 Mar;76(3):175–180. [PubMed] [Google Scholar]
  6. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  7. MUNOZ J. J. SYMPOSIUM ON RELATIONSHIP OF STRUCTURE OF MICROORGANISMS TO THEIR IMMUNOLOGICAL PROPERTIES. I. IMMUNOLOGICAL AND OTHER BIOLOGICAL ACTIVITIES OF BORDETELLA PERTUSSIS ANTIGENS. Bacteriol Rev. 1963 Dec;27:325–340. doi: 10.1128/br.27.4.325-340.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MUNOZ J., HESTEKIN B. M. Method of preparing histamine sensitizing factor. Nature. 1962 Dec 22;196:1192–1193. doi: 10.1038/1961192a0. [DOI] [PubMed] [Google Scholar]
  9. Morse S. I. The effect of hydrocortisone and x-irradiation on the lymphocytosis induced by Bordetella pertussis. J Exp Med. 1966 Feb 1;123(2):283–297. doi: 10.1084/jem.123.2.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Munoz J., Bergman R. K. Some histamine sensitizing properties of soluble preparations of the histamine sensitizing factor(HSF) from Bordetella pertussis. J Immunol. 1966 Jul;97(1):120–125. [PubMed] [Google Scholar]
  11. Nagel J. Isolation from Bordetella pertussis of protective antigen free from toxic activity and histamine sensitizing factor. Nature. 1967 Apr 1;214(5083):96–97. doi: 10.1038/214096a0. [DOI] [PubMed] [Google Scholar]
  12. Pieroni R. E., Broderick E. J., Levine L. The soluble protective antigen and the histamine-sensitizing factor of Bordetella pertussis. J Immunol. 1965 Oct;95(4):643–650. [PubMed] [Google Scholar]
  13. Preston N. W., Garrity P. Histamine-sensitising factor of Bordetella pertussis differentiated from immunogens by neutralisation and passive protection tests. J Pathol Bacteriol. 1967 Apr;93(2):483–492. doi: 10.1002/path.1700930209. [DOI] [PubMed] [Google Scholar]
  14. RONDLE C. J., MORGAN W. T. The determination of glucosamine and galactosamine. Biochem J. 1955 Dec;61(4):586–589. doi: 10.1042/bj0610586. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROWATT E. Some factors affecting the growth of Bordetella pertussis. J Gen Microbiol. 1957 Oct;17(2):279–296. doi: 10.1099/00221287-17-2-279. [DOI] [PubMed] [Google Scholar]
  16. ROWATT E. The growth of Bordetella pertussis: a review. J Gen Microbiol. 1957 Oct;17(2):297–326. doi: 10.1099/00221287-17-2-297. [DOI] [PubMed] [Google Scholar]
  17. SUTHERLAND I. W., WILKINSON J. F. A new growth medium for virulent Bordetella pertussis. J Pathol Bacteriol. 1961 Oct;82:431–438. doi: 10.1002/path.1700820220. [DOI] [PubMed] [Google Scholar]
  18. Sato Y., Nagase K. I. Isolation of protective antigen from Bordetella pertussis. Biochem Biophys Res Commun. 1967 Apr 20;27(2):195–201. doi: 10.1016/s0006-291x(67)80061-5. [DOI] [PubMed] [Google Scholar]

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