Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1969 Jun 1;129(6):1261–1273. doi: 10.1084/jem.129.6.1261

CELLS INVOLVED IN THE IMMUNE RESPONSE

VII. THE DEMONSTRATION, USING ALLOTYPIC MARKERS, OF ANTIBODY FORMATION BY IRRADIATION-RESISTANT CELLS OF IRRADIATED RABBITS INJECTED WITH NORMAL ALLOGENEIC BONE MARROW CELLS AND SHEEP ERYTHROCYTES

M Richter 1, N I Abdou 1
PMCID: PMC2138661  PMID: 4890861

Abstract

Bone marrow cells obtained from rabbits of one allotype were injected into irradiated rabbits of a different allotype. The recipients were also injected with sheep red blood cells, and their spleen cells were tested for plaque-forming capacity 7 days later. Spleen cells of all recipients gave large numbers of plaques as did spleen cells incubated with antiserum, directed toward donor allotype. However, incubation of the recipient spleen cells with antiserum directed toward recipient allotype completely suppressed plaque formation. These results demonstrate that antibody-formation in irradiated recipients of transferred lymphoid cells is a property of the recipient animal and that the antibody-forming cell is relatively irradiation-resistant. It was also demonstrated that only viable normal bone marrow cells are capable of transferring antibody-forming capacity to irradiated recipient rabbits. Neither sonicates nor heat-killed preparations of normal rabbit bone marrow cells possessed this capacity.

Full Text

The Full Text of this article is available as a PDF (729.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdou N. I., Richter M. Cells involved in the immune response. V. The migration of antigen-reactive immunocompetent cells out of the bone marrow following antigen administration. Int Arch Allergy Appl Immunol. 1969;35(4):330–334. [PubMed] [Google Scholar]
  2. Abdou N. I., Richter M. Cells involved in the immune response. VI. The immune response to red blood cells in irradiated rabbits after administration of normal, primed, or immune allogeneic rabbit bone marrow cells. J Exp Med. 1969 Apr 1;129(4):757–774. doi: 10.1084/jem.129.4.757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adler F. L., Fishman M., Dray S. Antibody formation initiated in vitro. 3. Antibody formation and allotypic specificity directed by ribonucleic acid from peritoneal exudate cells. J Immunol. 1966 Oct;97(4):554–558. [PubMed] [Google Scholar]
  4. Argyris B. F. Role of macrophages in antibody production. Immune response to sheep red blood cells. J Immunol. 1967 Oct;99(4):744–750. [PubMed] [Google Scholar]
  5. Chou C. T., Dubiski S., Cinader B. Cells as antigen carriers and as immunoglobulin producers. Synthesis of antibody and allogeneic immunoglobulin after transfer of antigen-treated cells to newborn rabbits. J Exp Med. 1967 Aug 1;126(2):305–330. doi: 10.1084/jem.126.2.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Claman H. N., Chaperon E. A., Triplett R. F. Immunocompetence of transferred thymus-marrow cell combinations. J Immunol. 1966 Dec;97(6):828–832. [PubMed] [Google Scholar]
  7. DRAY S., DUBISKI S., KELUS A., LENNOX E. S., OUDIN J. A notation for allotypy. Nature. 1962 Aug 25;195:785–786. doi: 10.1038/195785a0. [DOI] [PubMed] [Google Scholar]
  8. DRAY S., YOUNG G. O. Differences in the antigenic components of sera of individual rabbits as shown by induced isoprecipitins. J Immunol. 1958 Aug;81(2):142–149. [PubMed] [Google Scholar]
  9. DRAY S., YOUNG G. O., GERALD L. IMMUNOCHEMICAL IDENTIFICATION AND GENETICS OF RABBIT GAMMA-GLOBULIN ALLOTYPES. J Immunol. 1963 Sep;91:403–415. [PubMed] [Google Scholar]
  10. DRAY S., YOUNG G. O. Two antigenically different gamma-globulins in domestic rabbits revealed by isoprecipitins. Science. 1959 Apr 17;129(3355):1023–1025. doi: 10.1126/science.129.3355.1023. [DOI] [PubMed] [Google Scholar]
  11. DUBISKI S., DUDZIAK Z., SKALBA D. Serum groups in rabbits. Immunology. 1959 Jan;2(1):84–92. [PMC free article] [PubMed] [Google Scholar]
  12. DUBISKI S., SKALBA D., DUBISKA A., KELUS A. Iso-antigens of rabbit gamma-globulin. Nature. 1959 Dec 5;184(Suppl 23):1811–1812. doi: 10.1038/1841811a0. [DOI] [PubMed] [Google Scholar]
  13. FISHMAN M. Antibody formation in vitro. J Exp Med. 1961 Dec 1;114:837–856. doi: 10.1084/jem.114.6.837. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Gallily R., Feldman M. The role of macrophages in the induction of antibody in x-irradiated animals. Immunology. 1967 Feb;12(2):197–206. [PMC free article] [PubMed] [Google Scholar]
  15. Gottlieb A. A., Glisin V. R., Doty P. Studies on macrophage RNA involved in antibody production. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1849–1856. doi: 10.1073/pnas.57.6.1849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. HARRIS T. N., HARRIS S., BEALE H. D., SMITH J. J. Studies on the transfer of lymph node cells. IV. Effects of X-irradiation of recipient rabbits on the appearance of antibody after cell transfer. J Exp Med. 1954 Sep 1;100(3):289–300. doi: 10.1084/jem.100.3.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. HARRIS T. N., HARRIS S., FARBER M. B. Studies on the transfer of lymph node cells. XI. Effect on the anti-Shigella agglutinin titers of recipient rabbits of the prior injection of leucocytes from the donor animals. J Exp Med. 1958 Jul 1;108(1):21–36. doi: 10.1084/jem.108.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. HARRIS T. N., HARRIS S., FARBER M. B. Transfer of lymph node cells to recipient rabbits pre-injected with blood leucocytes of donors. Proc Soc Exp Biol Med. 1957 May;95(1):26–30. doi: 10.3181/00379727-95-23107. [DOI] [PubMed] [Google Scholar]
  19. Harris S., Harris T. N., Ogburn C. A. In vitro demonstration of suppressive antibody in mouse antisera vs. allogeneic spleen cells. J Immunol. 1967 Aug;99(2):447–453. [PubMed] [Google Scholar]
  20. Harris T. N., Harris S., Farber M. B. Suppression of transferred allogeneic spleen cells in the mouse: active induction and passive transfer by antiserum. J Immunol. 1967 Aug;99(2):438–446. [PubMed] [Google Scholar]
  21. JERNE N. K., NORDIN A. A. Plaque formation in agar by single antibody-producing cells. Science. 1963 Apr 26;140(3565):405–405. [PubMed] [Google Scholar]
  22. Kelus A. S., Gell P. G. Immunoglobulin allotypes of experimental animals. Prog Allergy. 1967;11:141–184. [PubMed] [Google Scholar]
  23. Lubaroff D. M., Waksman B. H. Bone marrow as a source of cells in reactions of cellular hypersensitivity. I. Passive transfer of tuberculin sensitivity in syngeneic systems. J Exp Med. 1968 Dec 1;128(6):1425–1435. doi: 10.1084/jem.128.6.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Lubaroff D. M., Waksman B. H. Bone marrow as source of cells in reactions of cellular hypersensitivity. II. Identification of allogeneic or hybrid cells by immunofluorescence in passively transferred tuberculin reactions. J Exp Med. 1968 Dec 1;128(6):1437–1447. doi: 10.1084/jem.128.6.1437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Mackaness G. B., Blanden R. V. Cellular immunity. Prog Allergy. 1967;11:89–140. [PubMed] [Google Scholar]
  26. McGregor D. D. Bone marrow origin of immunologically competent lymphocytes in the rat. J Exp Med. 1968 May 1;127(5):953–966. doi: 10.1084/jem.127.5.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Mitchell G. F., Miller J. F. Immunological activity of thymus and thoracic-duct lymphocytes. Proc Natl Acad Sci U S A. 1968 Jan;59(1):296–303. doi: 10.1073/pnas.59.1.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Mosier D. E., Coppleson L. W. A THREE-CELL INTERACTION REQUIRED FOR THE INDUCTION OF THE PRIMARY IMMUNE RESPONSE in vitro. Proc Natl Acad Sci U S A. 1968 Oct;61(2):542–547. doi: 10.1073/pnas.61.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. OUDIN J. Allotypy of rabbit serum proteins. II. Relationships between various allotypes: their common antigenic specificity, their distribution in a sample population; genetic implications. J Exp Med. 1960 Jul 1;112:125–142. doi: 10.1084/jem.112.1.125. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. OUDIN J. L'allotypie de certains antigènes protéidiques du sérum. C R Hebd Seances Acad Sci. 1956 May 23;242(21):2606–2608. [PubMed] [Google Scholar]
  33. OUDIN J. Réaction de précipitation spécifique entre des sérums d'animaux de même espèce. C R Hebd Seances Acad Sci. 1956 May 14;242(20):2489–2490. [PubMed] [Google Scholar]
  35. Pearsall N. N., Weiser R. S. The macrophage in allograft immunity. II. Passive transfer with immune macrophages. J Reticuloendothel Soc. 1968 Apr;5(2):121–133. [PubMed] [Google Scholar]
  36. Pribnow J. F., Silverman M. S. Studies on the radiosensitive phase of the primary antibody response in rabbits. I. The role of the macrophage. J Immunol. 1967 Feb;98(2):225–229. [PubMed] [Google Scholar]
  37. Robinson W. A., Marbrook J., Diener E. Primary stimulation and measurement of antibody production to sheep red blood cells in vitro. J Exp Med. 1967 Aug 1;126(2):347–356. doi: 10.1084/jem.126.2.347. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. SELL S., GELL P. G. STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM. J Exp Med. 1965 Aug 1;122:423–440. doi: 10.1084/jem.122.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Sell S., Gell P. G. Studies on rabbit lymphocytes in vitro. IV. Blast transformation of the lymphocytes from newborn rabbits induced by antiallotype serum to a paternal IgG allotype not present in the serum of the lymphocyte donors. J Exp Med. 1965 Nov 1;122(5):923–928. doi: 10.1084/jem.122.5.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Sell S. Immunoglobulin M allotypes of the rabbit: identification of a second specificity. Science. 1966 Aug 5;153(3736):641–643. doi: 10.1126/science.153.3736.641. [DOI] [PubMed] [Google Scholar]
  41. Singhal K., Richter M. Cells involved in the immune response. I. The response of normal rabbit bone marrow cells to antigens in vitro. Int Arch Allergy Appl Immunol. 1968;33(5):493–500. doi: 10.1159/000230064. [DOI] [PubMed] [Google Scholar]
  42. Singhal S. K., Richter M. Cells involved in the immune response. IV. The response of normal and immune rabbit bone marrow and lymphoid tissue lymphocytes to antigens in vitro. J Exp Med. 1968 Nov 1;128(5):1099–1128. doi: 10.1084/jem.128.5.1099. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Singhal S. K., Richter M., Osmond D. G. Cells involved in the immune response. 3. Responsiveness in antigens of a lymphocyte-rich fraction of normal rabbit bone marrow. Int Arch Allergy Appl Immunol. 1968;34(3):224–232. [PubMed] [Google Scholar]
  44. Virolainen M. Hematopoietic origin of macrophages as studied by chromosome markers in mice. J Exp Med. 1968 May 1;127(5):943–952. doi: 10.1084/jem.127.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Volkman A. The origin and turnover of mononuclear cells in peritoneal exudates in rats. J Exp Med. 1966 Aug 1;124(2):241–254. doi: 10.1084/jem.124.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Walsh P., Maurer P., Egan M. Detection of immune response against synthetic polymers of amino acids employing the plaque-forming cell system. I. Reaction with sheep erythrocytes coated with polymer. J Immunol. 1967 Feb;98(2):344–350. [PubMed] [Google Scholar]
  47. Weiler E., Melletz E. W., Breuninger-Peck E. Facilitation of immune hemolysis by an interaction between red cell-sensitizing antibody and gamma-globulin allotype antibody. Proc Natl Acad Sci U S A. 1965 Nov;54(5):1310–1317. doi: 10.1073/pnas.54.5.1310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES