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. 1969 Jun 1;129(6):1131–1143. doi: 10.1084/jem.129.6.1131

THE REQUIREMENT OF MORE THAN ONE ANTIGENIC DETERMINANT FOR IMMUNOGENICITY

K Rajewsky 1, V Schirrmacher 1, S Nase 1, N K Jerne 1
PMCID: PMC2138664  PMID: 4181830

Abstract

Rabbits primarily stimulated with a BSA (bovine serum albumin)-sulfanilic acid complex will produce a good secondary response to the sulfanilic acid hapten if the carrier used in the secondary stimulus is again BSA, and not if the secondary carrier is HGG (human gamma globulin). In the latter situation, a good secondary response is obtained, however, if the rabbits are pretreated a few weeks earlier with free HGG. We conclude that the immune stimulus involves the recognition of carrier determinants unrelated to the hapten. As the receptors for recognition of unrelated determinants are probably situated on different cells, we suggest that the immune stimulus leading to antibody formation requires the interaction of two antigen-bridged cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASHLEY H., OVARY Z. EFFECT OF BOVINE GAMMA GLOBULIN ON SUBSEQUENT IMMUNIAZTION WITH DINITROPHENYLATED BOVINE GAMMA GLOBULIN. Proc Soc Exp Biol Med. 1965 Jun;119:311–314. doi: 10.3181/00379727-119-30165. [DOI] [PubMed] [Google Scholar]
  2. BULLOCK W. E., KANTOR F. S. HEMAGGLUTINATION REACTIONS OF HUMAN ERYTHROCYTES CONJUGATED COVALENTLY WITH DINITROPHENYL GROUPS. J Immunol. 1965 Mar;94:317–322. [PubMed] [Google Scholar]
  3. Claman H. N., Chaperon E. A., Triplett R. F. Thymus-marrow cell combinations. Synergism in antibody production. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1167–1171. doi: 10.3181/00379727-122-31353. [DOI] [PubMed] [Google Scholar]
  4. DIXON F. J., MAURER P. H. Specificity of the secondary response to protein antigens. J Immunol. 1955 Jun;74(6):418–431. [PubMed] [Google Scholar]
  5. Davies A. J., Leuchars E., Wallis V., Koller P. C. The mitotic response of thymus-derived cells to antigenic stimulus. Transplantation. 1966 Jul;4(4):438–451. doi: 10.1097/00007890-196607000-00008. [DOI] [PubMed] [Google Scholar]
  6. EISEN H. N., CARSTEN M. E., BELMAN S. Studies of hypersensitivity to low molecular weight substances. III. The 2,4-dinitrophenyl group as a determinant in the preciptin reaction. J Immunol. 1954 Nov;73(5):296–308. [PubMed] [Google Scholar]
  7. Gallily R., Feldman M. The role of macrophages in the induction of antibody in x-irradiated animals. Immunology. 1967 Feb;12(2):197–206. [PMC free article] [PubMed] [Google Scholar]
  8. Green I., Paul W. E., Benacerraf B. Hapten carrier relationships in the DNP-PLL foreign albumin complex system: induction of tolerance and stimulation of cells in vitro. J Exp Med. 1968 Jan 1;127(1):43–53. doi: 10.1084/jem.127.1.43. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. INGRAHAM J. S. [Specific, complement-dependent hemolysis of sheep erythrocytes by antiserum to azo hapten groups]. J Infect Dis. 1952 Nov-Dec;91(3):268–275. doi: 10.1093/infdis/91.3.268. [DOI] [PubMed] [Google Scholar]
  10. Levine B. B. Specificity of the anamnestic response to a double hapten conjugate in guinea pigs primed with a single hapten. J Immunol. 1967 Dec;99(6):1173–1177. [PubMed] [Google Scholar]
  11. Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mosier D. E. A requirement for two cell types for antibody formation in vitro. Science. 1967 Dec 22;158(3808):1573–1575. doi: 10.1126/science.158.3808.1573. [DOI] [PubMed] [Google Scholar]
  14. Paul W. E., Siskind G. W., Benacerraf B., Ovary Z. Secondary antibody responses in haptenic systems: cell population selection by antigen. J Immunol. 1967 Oct;99(4):760–770. [PubMed] [Google Scholar]
  15. Pinchuck P., Maurer P. H. Antigenicity of polypeptides (poly alpha amino acids). XVI. Genetic control of immunogenicity of synthetic polypeptides in mice. J Exp Med. 1965 Oct 1;122(4):673–679. doi: 10.1084/jem.122.4.673. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rajewsky K. Kreuzreagierende antigene Determinanten auf Lactatdehydrogenasen I und V durch Acetylierung. Biochim Biophys Acta. 1966 May 26;121(1):51–68. [PubMed] [Google Scholar]
  17. Rajewsky K., Rottländer E., Peltre G., Müller B. The immune response to a hybrid protein molecule; specificity of secondary stimulation and of tolerance induction. J Exp Med. 1967 Oct 1;126(4):581–606. doi: 10.1084/jem.126.4.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SALVIN S. B., SMITH R. F. Specificity of allergic reactions. II. Azoproteins in the anamnestic response. Proc Soc Exp Biol Med. 1960 Aug-Sep;104:584–590. doi: 10.3181/00379727-104-25916. [DOI] [PubMed] [Google Scholar]
  19. SELL S., GELL P. G. STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM. J Exp Med. 1965 Aug 1;122:423–440. doi: 10.1084/jem.122.2.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schierman L. W., McBride R. A. Adjuvant activity of erythrocyte isoantigens. Science. 1967 May 5;156(3775):658–659. doi: 10.1126/science.156.3775.658. [DOI] [PubMed] [Google Scholar]
  21. Sell S. Studies on rabbit lymphocytes in vitro. V. The induction of blast transformation with sheep antisera to rabbit IgG subunits. J Exp Med. 1967 Feb 1;125(2):289–301. doi: 10.1084/jem.125.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sell S. Studies on rabbit lymphocytes in vitro. VI. The induction of blast transformation with sheep antisera to rabbit IgA and IgM. J Exp Med. 1967 Mar 1;125(3):393–400. doi: 10.1084/jem.125.3.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Steiner L. A., Eisen H. N. The relative affinity of antibodies synthesized in the secondary response. J Exp Med. 1967 Dec 1;126(6):1185–1205. doi: 10.1084/jem.126.6.1185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Unanue E. R., Askonas B. A. The immune response of mice to antigen in macrophages. Immunology. 1968 Aug;15(2):287–296. [PMC free article] [PubMed] [Google Scholar]
  25. van Furth R., Schuit H. R., Hijmans W. The formation of immunoglobulins by human tissues in vitro. IV. Circulating lymphocytes in normal and pathological conditions. Immunology. 1966 Jul;11(1):29–40. [PMC free article] [PubMed] [Google Scholar]

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