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. 1987 Oct;169(10):4848–4851. doi: 10.1128/jb.169.10.4848-4851.1987

Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp.

Y Wang 1, I Mahler 1, H S Levinson 1, H O Halvorson 1
PMCID: PMC213867  PMID: 2820946

Abstract

A 7.9-kilobase (kb) chromosomal fragment was cloned from a mercury-resistant Bacillus sp. In Escherichia coli, in the presence of a second plasmid carrying functional transport genes, resistance to HgCl2 and to phenylmercury acetate (PMA) was expressed. Shortening the cloned fragment to 3.8 kb abolished resistance to PMA but not to HgCl2. In Bacillus subtilis, the 3.8-kb fragment produced mercuric reductase constitutively but did not produce resistance to HgCl2 or to PMA.

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Selected References

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