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. 1987 Oct;169(10):4857–4860. doi: 10.1128/jb.169.10.4857-4860.1987

Structure and organization of the hisA gene of the thermophilic archaebacterium Methanococcus thermolithotrophicus.

C F Weil 1, G S Beckler 1, J N Reeve 1
PMCID: PMC213870  PMID: 3115964

Abstract

A restriction fragment of Methanococcus thermolithotrophicus genomic DNA was cloned into pUC8 to produce plasmid pET9301, which complements mutations in the hisA gene of Escherichia coli. Sequencing the DNA (2,155 base pairs) cloned from this thermophilic methanogen demonstrated that the M. thermolithotrophicus hisA gene is located within a cluster of open reading frames (ORFs) and is 68 and 69% homologous at the nucleotide level to the hisA genes of the mesophilic methanococci M. voltae and M. vannielii, respectively. The ORF (ORF 206) immediately 5' to the hisA gene of M. thermolithotrophicus is partially deleted in the genomes of the two mesophilic species, whereas ORF 114, which is 5' to ORF 206, is conserved in all three species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. Methanogens: reevaluation of a unique biological group. Microbiol Rev. 1979 Jun;43(2):260–296. doi: 10.1128/mr.43.2.260-296.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beckler G. S., Reeve J. N. Conservation of primary structure in the hisI gene of the archaebacterium, Methanococcus vannielii, the eubacterium Escherichia coli, and the eucaryote Saccharomyces cerevisiae. Mol Gen Genet. 1986 Jul;204(1):133–140. doi: 10.1007/BF00330200. [DOI] [PubMed] [Google Scholar]
  3. Bertani G., Baresi L. Genetic transformation in the methanogen Methanococcus voltae PS. J Bacteriol. 1987 Jun;169(6):2730–2738. doi: 10.1128/jb.169.6.2730-2738.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chiariotti L., Alifano P., Carlomagno M. S., Bruni C. B. Nucleotide sequence of the Escherichia coli hisD gene and of the Escherichia coli and Salmonella typhimurium hisIE region. Mol Gen Genet. 1986 Jun;203(3):382–388. doi: 10.1007/BF00422061. [DOI] [PubMed] [Google Scholar]
  5. Cue D., Beckler G. S., Reeve J. N., Konisky J. Structure and sequence divergence of two archaebacterial genes. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4207–4211. doi: 10.1073/pnas.82.12.4207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Klein A., Schnorr M. Genome complexity of methanogenic bacteria. J Bacteriol. 1984 May;158(2):628–631. doi: 10.1128/jb.158.2.628-631.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
  8. Reeve A., Shulman S. A., Zimmerman A. W., Cassidy S. B. Methylphenidate therapy for aggression in a man with ring 22 chromosome. Report and literature review. Arch Neurol. 1985 Jan;42(1):69–72. doi: 10.1001/archneur.1985.04060010075019. [DOI] [PubMed] [Google Scholar]
  9. STADTMAN T. C., BARKER H. A. Studies on the methane fermentation. X. A new formate-decomposing bacterium, Methanococcus vannielii. J Bacteriol. 1951 Sep;62(3):269–280. doi: 10.1128/jb.62.3.269-280.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Santoro N., Konisky J. Characterization of bromoethanesulfonate-resistant mutants of Methanococcus voltae: evidence of a coenzyme M transport system. J Bacteriol. 1987 Feb;169(2):660–665. doi: 10.1128/jb.169.2.660-665.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  12. Whitman W. B., Ankwanda E., Wolfe R. S. Nutrition and carbon metabolism of Methanococcus voltae. J Bacteriol. 1982 Mar;149(3):852–863. doi: 10.1128/jb.149.3.852-863.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Woese C. R., Olsen G. J. Archaebacterial phylogeny: perspectives on the urkingdoms. Syst Appl Microbiol. 1986;7:161–177. doi: 10.1016/s0723-2020(86)80001-7. [DOI] [PubMed] [Google Scholar]
  14. Wood A. G., Whitman W. B., Konisky J. A newly-isolated marine methanogen harbors a small cryptic plasmid. Arch Microbiol. 1985 Aug;142(3):259–261. doi: 10.1007/BF00693400. [DOI] [PubMed] [Google Scholar]

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