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. 1969 Sep 1;130(3):519–541. doi: 10.1084/jem.130.3.519

CROSSING OVER BETWEEN GENES IN THE IMMUNOGLOBULIN HEAVY CHAIN LINKAGE GROUP OF THE MOUSE

Rose Lieberman 1, Michael Potter 1
PMCID: PMC2138713  PMID: 4185248

Abstract

Immunoglobulin heavy chain genes were found in wild mice (Mus musculus) that could best be explained as recombinants of immunoglobulin genotypes. In wild mice from Kitty Hawk, N. C., two new heavy chain linkage groups, G3,5,7,8H9,11FfA- and G3,5,8H9,11FfA-, were found, each of which genetically controls both the 3 and 5 distinct immunoglobulin determinants. In inbred strains the 3 and 5 determinants are found independently. The new heavy chain allotype G3,5,7,8H9,11FfA- probably arose from a homologous (intragenic) cross-over between G3,8H9,11FfA- and G5,7,8H9,11FfA14 heavy chain linkage groups. It was suggested that genes controlling G3,8G5,7,8, G3,5,8, and G3,5,7,8 are alleles. Another homozygous wild mouse (Kyushu, Japan) showed a new heavy chain allotype, 2G1,6,7,8H9,16FsA15. The 2 and G1,6,7,8 determinants are also separated in inbred strains. The 2 determinant in inbred mice is not on the γF, γH, or γA heavy chain and is probably on a γG or γG-like immunoglobulin heavy chain. Papain digestion of serum from the Kyushu mouse showed two electrophoretically different Fc fragments, one carrying the G1,6,7,8 and the other the 2 determinant. The new heavy chain allotype, 2G1,6,7,8H9,16FsA15, of the Kyushu wild mouse probably arose from a nonhomologous (unequal) cross over between 2G-H9,16FsA15 and G1,6,7,8H9,11FfA12,13,14 heavy chain linkage groups. The linkage group of the Kyushu wild mouse has at least five heavy chain genes, while that of the inbred mice has four.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barth W. F., McLaughlin C. L., Fahey J. L. The immunoglobulins of mice. VI. Response to immunization. J Immunol. 1965 Nov;95(5):781–790. [PubMed] [Google Scholar]
  2. Coe J. E. 7Sgamma-1 and 7Sgamma-2 antibody response in the mouse. I. Influence of strain, antigen and adjuvant. J Immunol. 1966 Apr;96(4):744–753. [PubMed] [Google Scholar]
  3. Dreyer W. J., Bennett J. C. The molecular basis of antibody formation: a paradox. Proc Natl Acad Sci U S A. 1965 Sep;54(3):864–869. doi: 10.1073/pnas.54.3.864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. FAHEY J. L., SELL S. THE IMMUNOGLOBULINS OF MICE. V. THE METABOLIC (CATABOLIC) PROPERTIES OF FIVE IMMUNOGLOBULIN CLASSES. J Exp Med. 1965 Jul 1;122:41–58. doi: 10.1084/jem.122.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. FAHEY J. L., WUNDERLICH J., MISHELL R. THE IMMUNOGLOBULINS OF MICE. I. FOUR MAJOR CLASSES OF IMMUNOGLOBULINS: 7S GAMMA-2-, 7S GAMMA-1-, GAMMA-1A (BETA-2A)-, AND 18S GAMMA-1M-GLOBULINS. J Exp Med. 1964 Aug 1;120:223–242. doi: 10.1084/jem.120.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HERZENBERG L. A. A CHROMOSOME REGION FOR GAMMA 2A AND BETA 2A GLOBULIN H CHAIN ISOANTIGENS IN THE MOUSE. Cold Spring Harb Symp Quant Biol. 1964;29:455–462. doi: 10.1101/sqb.1964.029.01.048. [DOI] [PubMed] [Google Scholar]
  7. Hood L., Ein D. Immunologlobulin lambda chain structure: two genes, one polypeptide chain. Nature. 1968 Nov 23;220(5169):764–767. doi: 10.1038/220764a0. [DOI] [PubMed] [Google Scholar]
  8. Lieberman R., Potter M. Close linkage in genes controlling gamma-A and gamma-G heavy chain structure in BALB/c mice. J Mol Biol. 1966 Jul;18(3):516–528. doi: 10.1016/s0022-2836(66)80040-2. [DOI] [PubMed] [Google Scholar]
  9. Lieberman R., Potter M. Polymorphism of heavy-chain genes in immunoglobulins of wild mice. Science. 1966 Oct 28;154(3748):535–537. doi: 10.1126/science.154.3748.535. [DOI] [PubMed] [Google Scholar]
  10. Litwin S. D., Kunkel H. G. The genetic control of gamma-globulin heavy chains. Studies of the major heavy chain subgroup utilizing multiple genetic markers. J Exp Med. 1967 May 1;125(5):847–862. doi: 10.1084/jem.125.5.847. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. MERRYMAN C., BENACERRAF B. STUDIES ON THE STRUCTURE OF MOUSE ANTIBODIES. Proc Soc Exp Biol Med. 1963 Nov;114:372–376. doi: 10.3181/00379727-114-28681. [DOI] [PubMed] [Google Scholar]
  12. NUSSENZWEIG R. S., MERRYMAN C., BENACERRAF B. ELECTROPHORETIC SEPARATION AND PROPERTIES OF MOUSE ANTIHAPTEN ANTIBODIES INVOLVED IN PASSIVE CUTANEOUS ANAPHYLAXIS AND PASSIVE HEMOLYSIS. J Exp Med. 1964 Aug 1;120:315–328. doi: 10.1084/jem.120.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Natvig J. B., Kunkel H. G., Yount W. J., Nielsen J. C. FURTHER STUDIES ON THE gammaG-HEAVY CHAIN GENE COMPLEXES, WITH PARTICULAR REFERENCE TO THE GENETIC MARKERS Gm(g) AND Gm(n). J Exp Med. 1968 Oct 1;128(4):763–784. doi: 10.1084/jem.128.4.763. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Porter R. R. The structure of the heavy chain of immunoglobulin and its relevance to the nature of the antibody-combining site. The Second CIBA Medal Lecture. Biochem J. 1967 Nov;105(2):417–426. doi: 10.1042/bj1050417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Potter M., Appella E., Geisser S. Variations in the heavy polypeptide chain structure of gamma myeloma immunoglobulins from an inbred strain of mice and a hypothesis as to their origin. J Mol Biol. 1965 Dec;14(2):361–372. doi: 10.1016/s0022-2836(65)80187-5. [DOI] [PubMed] [Google Scholar]
  16. Potter M., Lieberman R., Dray S. Isoantibodies specific for myeloma gamma-G and gamma-H immunoglobulins of BALB-c mice. J Mol Biol. 1966 Apr;16(2):334–346. doi: 10.1016/s0022-2836(66)80177-8. [DOI] [PubMed] [Google Scholar]
  17. Potter M., Lieberman R. Genetics of immunoglobulins in the mouse. Adv Immunol. 1967;7:91–145. doi: 10.1016/s0065-2776(08)60127-3. [DOI] [PubMed] [Google Scholar]
  18. Prahl J. W. The C-terminal sequences of the heavy chains of human immunoglobulin G myeloma proteins of differing isotopes and allotypes. Biochem J. 1967 Dec;105(3):1019–1028. doi: 10.1042/bj1051019. [DOI] [PMC free article] [PubMed] [Google Scholar]

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