Abstract
Thoracic duct cells and spleen cells were tested for their ability to restore the primary antibody response of X-irradiated rats to bovine serum albumin (BSA), sheep red blood cells (SRBC), horse spleen femtin (HSF), and Salmonella typhi flagella. Spleen cells were at least as efficient as thoracic duct cells in restoring the response to BSA, HSF, and Salmonella typhi flagella. In further experiments thoracic duct cells lacking large dividing lymphocytes were tested for their ability to restore the primary response. Large lymphocytes were eliminated by the in vitro incubation of thoracic duct cells for 24 hr at 37°C or by treatment of thoracic duct cell donors with the mitotic inhibitor vinblastine sulfate 24 hr prior to cannulation of the thoracic duct. Experiments with SRBC show that incubated cells and cells from vinblastine-treated donors are as efficient as normal cells in restoring the primary antibody response. On the other hand, experiments with HSF and Salmonella typhi flagella show that incubated cells and cells from vinblastine-treated donors are about five times less efficient than normal cells in restoring the response. Normal thoracic duct cells were more efficient than incubated cells but less efficient than cells from vinblastine-treated donors in restoring the early response to BSA. The experimental findings indicate that the classes of thoracic duct lymphocytes which initiate the primary antibody response to SRBC differ from the classes which initiate the response to HSF and Salmonella typhi flagella, or BSA.
Full Text
The Full Text of this article is available as a PDF (625.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ada G. L., Byrt P. Specific inactivation of antigen-reactive cells with 125I-labelled antigen. Nature. 1969 Jun 28;222(5200):1291–1292. doi: 10.1038/2221291a0. [DOI] [PubMed] [Google Scholar]
- CAFFREY R. W., RIEKE W. O., EVERETT N. B. Radioautographic studies of small lymphocytes in the thoracic duct of the rat. Acta Haematol. 1962;28:145–154. doi: 10.1159/000207257. [DOI] [PubMed] [Google Scholar]
- GOWANS J. L., KNIGHT E. J. THE ROUTE OF RE-CIRCULATION OF LYMPHOCYTES IN THE RAT. Proc R Soc Lond B Biol Sci. 1964 Jan 14;159:257–282. doi: 10.1098/rspb.1964.0001. [DOI] [PubMed] [Google Scholar]
- GOWANS J. L., McGREGOR D. D., COWEN D. M. Initiation of immune responses by small lymphocytes. Nature. 1962 Nov 17;196:651–655. doi: 10.1038/196651a0. [DOI] [PubMed] [Google Scholar]
- Gowans J. L., Uhr J. W. The carriage of immunological memory by small lymphocytes in the rat. J Exp Med. 1966 Nov 1;124(5):1017–1030. doi: 10.1084/jem.124.5.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KOBAYASHI T., RINKER J. N., KOFFLER H. Purification and and chemical properties of flagellin. Arch Biochem Biophys. 1959 Oct;84:342–362. doi: 10.1016/0003-9861(59)90598-3. [DOI] [PubMed] [Google Scholar]
- McGregor D. D., Gowans J. L. THE ANTIBODY RESPONSE OF RATS DEPLETED OF LYMPHOCYTES BY CHRONIC DRAINAGE FROM THE THORACIC DUCT. J Exp Med. 1963 Jan 31;117(2):303–320. doi: 10.1084/jem.117.2.303. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McGregor D. D., McCullagh P. J., Gowans J. L. The role of lymphocytes in antibody formation. I. Restoration of the haemolysin response in x-irradiated rats with lymphocytes from normal and immunologically tolerant donors. Proc R Soc Lond B Biol Sci. 1967 Sep 12;168(1012):229–243. doi: 10.1098/rspb.1967.0063. [DOI] [PubMed] [Google Scholar]
- Nossal G. J., Cunningham A., Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. 3. Chromosomal marker analysis of single antibody-forming cells in reconstituted, irradiated, or thymectomized mice. J Exp Med. 1968 Oct 1;128(4):839–853. doi: 10.1084/jem.128.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
- Strober S. Initiation of primary antibody responses by both circulating and non-circulating lymphocytes. Nature. 1968 Aug 10;219(5154):649–651. doi: 10.1038/219649a0. [DOI] [PubMed] [Google Scholar]
- Strober S., Law L. W. Further studies on the role of circulating lymphocytes in the initiation of primary antibody responses to different antigens. Proc Natl Acad Sci U S A. 1969 Apr;62(4):1023–1030. doi: 10.1073/pnas.62.4.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strober S., Mandel M. A. Differences in the distribution of antigen reactive cells in the lymphoid tissues of the rat and mouse. Proc Soc Exp Biol Med. 1969 Jan;130(1):336–338. doi: 10.3181/00379727-130-33550. [DOI] [PubMed] [Google Scholar]
- Syeklocha D., Siminovitch L., Till J. E., McCulloch E. A. The proliferative state of antigen-sensitive precursors of hemolysin-producing cells, determined by the use of the inhibitor, vinblastine. J Immunol. 1966 Mar;96(3):472–477. [PubMed] [Google Scholar]