Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1970 Aug 1;132(2):353–367. doi: 10.1084/jem.132.2.353

ANTIGEN-SPECIFIC SYNERGISM IN THE IMMUNE RESPONSE OF IRRADIATED MICE GIVEN MARROW CELLS AND PERITONEAL CAVITY CELLS OR EXTRACTS

James C Kennedy 1, Perry E Treadwell 1, Edwin S Lennox 1
PMCID: PMC2138736  PMID: 4927598

Abstract

A synergistic immune response to foreign erythrocytes may be induced in heavily irradiated mice injected with a mixture of isologous cells obtained from marrow and from the peritoneal cavity. Under appropriate conditions, homologous or heterologous peritoneal cavity cells, heat-killed cells, or cellfree extracts made from such cells are also effective. The activity of the peritoneal cavity cells or extracts is antigen-specific, in the sense that cells or extracts obtained from animals previously immunized with the test antigen produce much stronger synergistic effects than do cells from animals immunized with some other antigen; however, the peritoneal cavity cells or extracts are not immunogenic when tested in primed animals. The marrow cells, demonstrated to contain precursors of the antibody-forming cells produced during this synergistic immune response, also show a form of antigen-specificity.

Full Text

The Full Text of this article is available as a PDF (820.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ASKONAS B. A., RHODES J. M. IMMUNOGENICITY OF ANTIGEN-CONTAINING RIBONUCLEIC ACID PREPARATIONS FROM MACROPHAGES. Nature. 1965 Jan 30;205:470–474. doi: 10.1038/205470a0. [DOI] [PubMed] [Google Scholar]
  2. Adler F. L., Fishman M., Dray S. Antibody formation initiated in vitro. 3. Antibody formation and allotypic specificity directed by ribonucleic acid from peritoneal exudate cells. J Immunol. 1966 Oct;97(4):554–558. [PubMed] [Google Scholar]
  3. Albright J. F., Omer T. F., Deitchman J. W. Antigen competition: antigens compete for a cell occurring with limited frequency. Science. 1970 Jan 9;167(3915):196–198. doi: 10.1126/science.167.3915.196. [DOI] [PubMed] [Google Scholar]
  4. Argyris B. F. Role of macrophages in antibody production. Immune response to sheep red blood cells. J Immunol. 1967 Oct;99(4):744–750. [PubMed] [Google Scholar]
  5. Bell C., Dray S. Conversion of non-immune spleen cells by ribonucleic acid of lymphoid cells from an immunized rabbit to produce gammaM antibody of foreign light chain allotype. J Immunol. 1969 Dec;103(6):1196–1211. [PubMed] [Google Scholar]
  6. Braun W., Nakano M. Antibody formation: stimulation by polyadenylic and polycytidylic acids. Science. 1967 Aug 18;157(3790):819–821. doi: 10.1126/science.157.3790.819. [DOI] [PubMed] [Google Scholar]
  7. Bretscher P. A., Cohn M. Minimal model for the mechanism of antibody induction and paralysis by antigen. Nature. 1968 Nov 2;220(5166):444–448. doi: 10.1038/220444a0. [DOI] [PubMed] [Google Scholar]
  8. Claman H. N., Chaperon E. A., Triplett R. F. Immunocompetence of transferred thymus-marrow cell combinations. J Immunol. 1966 Dec;97(6):828–832. [PubMed] [Google Scholar]
  9. Claman H. N., Chaperon E. A., Triplett R. F. Thymus-marrow cell combinations. Synergism in antibody production. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1167–1171. doi: 10.3181/00379727-122-31353. [DOI] [PubMed] [Google Scholar]
  10. Friedman H. P., Stavitsky A. B., Solomon J. M. Induction in vitro of antibodies to phage T2: antigens in the RNA extract employed. Science. 1965 Sep 3;149(3688):1106–1107. doi: 10.1126/science.149.3688.1106. [DOI] [PubMed] [Google Scholar]
  11. Haskill J. S., Byrt P., Marbrook J. In vitro and in vivo studies of the immune response to sheep erythrocytes using partially purified cell preparations. J Exp Med. 1970 Jan 1;131(1):57–76. doi: 10.1084/jem.131.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kornfeld L., Weyzen W. W. Antibody formation by transferred peritoneal cells and spleen cells of mice. I. Transfer of cells from immunized non-irradiated donors to syngeneic recipients with and without antigen. Immunology. 1968 Nov;15(5):751–764. [PMC free article] [PubMed] [Google Scholar]
  13. Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mitchell G. F., Miller J. F. Immunological activity of thymus and thoracic-duct lymphocytes. Proc Natl Acad Sci U S A. 1968 Jan;59(1):296–303. doi: 10.1073/pnas.59.1.296. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Mosier D. E. A requirement for two cell types for antibody formation in vitro. Science. 1967 Dec 22;158(3808):1573–1575. doi: 10.1126/science.158.3808.1573. [DOI] [PubMed] [Google Scholar]
  17. Mosier D. E. Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters. J Exp Med. 1969 Feb 1;129(2):351–362. doi: 10.1084/jem.129.2.351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mosier D. E., Coppleson L. W. A THREE-CELL INTERACTION REQUIRED FOR THE INDUCTION OF THE PRIMARY IMMUNE RESPONSE in vitro. Proc Natl Acad Sci U S A. 1968 Oct;61(2):542–547. doi: 10.1073/pnas.61.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mosier D. E., Fitch F. W., Rowley D. A., Davies A. J. Cellular deficit in thymectomized mice. Nature. 1970 Jan 17;225(5229):276–277. doi: 10.1038/225276a0. [DOI] [PubMed] [Google Scholar]
  20. Munro A., Hunter P. In vitro reconstitution of the immune response of thymus-deprived mice to sheep red blood cells. Nature. 1970 Jan 17;225(5229):277–278. doi: 10.1038/225277a0. [DOI] [PubMed] [Google Scholar]
  21. Pierce C. W., Benacerraf B. Immune response in vitro: independence of "activated" lymphoid cells. Science. 1969 Nov 21;166(3908):1002–1004. doi: 10.1126/science.166.3908.1002. [DOI] [PubMed] [Google Scholar]
  22. Radovich J., Hemingsen H., Talmage D. W. The enhancing effect of bone marrow cells on the immune response of irradiated mice reconstituted with spleen cells from normal and immunized donors. J Immunol. 1968 Apr;100(4):756–759. [PubMed] [Google Scholar]
  23. Raidt D. J., Mishell R. I., Dutton R. W. Cellular events in the immune response : analysis and in vitro response of mouse spleen cell populations separated by differential flotation in albumin gradients. J Exp Med. 1968 Oct 1;128(4):681–698. doi: 10.1084/jem.128.4.681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Unanue E. R., Askonas B. A. The immune response of mice to antigen in macrophages. Immunology. 1968 Aug;15(2):287–296. [PMC free article] [PubMed] [Google Scholar]
  25. VOGT M., DULBECCO R. Steps in the neoplastic transformation of hamster embryo cells by polyoma virus. Proc Natl Acad Sci U S A. 1963 Feb 15;49:171–179. doi: 10.1073/pnas.49.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES