Abstract
Macrophages were cultured for several hours after a brief exposure to radio-iodinated keyhole limpet hemocyanin. Most of the hemocyanin taken up by the macrophages was rapidly catabolized and eliminated from the cell. A few molecules were retained on the plasma membrane of the cells for prolonged periods and were not subject to endocytosis and catabolism. These few molecules of hemocyanin bound to the plasma membrane were identified by observing the fixation of antibody fragments to macrophages at low temperature. The membrane-bound antigen, which could be removed by trypsin or EDTA, was of large molecular size, though heterogeneous. A great part of the immune responses of mice to hemocyanin bound to live macrophages could be abrogated by treatment of the macrophages in vitro with antibody or trypsin. Hence, most of the immunogenicity of hemocyanin bound to macrophages was attributed to the few molecules of antigen bound to the plasma membrane.
Full Text
The Full Text of this article is available as a PDF (841.6 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ASKONAS B. A., RHODES J. M. IMMUNOGENICITY OF ANTIGEN-CONTAINING RIBONUCLEIC ACID PREPARATIONS FROM MACROPHAGES. Nature. 1965 Jan 30;205:470–474. doi: 10.1038/205470a0. [DOI] [PubMed] [Google Scholar]
- Breit G. Virtual coulomb excitation in nucleon transfer. Proc Natl Acad Sci U S A. 1967 Apr;57(4):849–855. doi: 10.1073/pnas.57.4.849. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cerottini J. C., McConahey P. J., Dixon F. J. Specificity of the immunosuppression caused by passive administration of antibody. J Immunol. 1969 Aug;103(2):268–275. [PubMed] [Google Scholar]
- Cohn Z. A. The structure and function of monocytes and macrophages. Adv Immunol. 1968;9:163–214. doi: 10.1016/s0065-2776(08)60443-5. [DOI] [PubMed] [Google Scholar]
- FARR R. S. A quantitative immunochemical measure of the primary interaction between I BSA and antibody. J Infect Dis. 1958 Nov-Dec;103(3):239–262. doi: 10.1093/infdis/103.3.239. [DOI] [PubMed] [Google Scholar]
- Friedman H. P., Stavitsky A. B., Solomon J. M. Induction in vitro of antibodies to phage T2: antigens in the RNA extract employed. Science. 1965 Sep 3;149(3688):1106–1107. doi: 10.1126/science.149.3688.1106. [DOI] [PubMed] [Google Scholar]
- Gallily R., Feldman M. The role of macrophages in the induction of antibody in x-irradiated animals. Immunology. 1967 Feb;12(2):197–206. [PMC free article] [PubMed] [Google Scholar]
- McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
- Nossal G. J., Abbot A., Mitchell J. Antigens in immunity. XIV. Electron microscopic radioautographic studies of antigen capture in the lymph node medulla. J Exp Med. 1968 Feb 1;127(2):263–276. doi: 10.1084/jem.127.2.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roelants G. E., Goodman J. W. The chemical nature of macrophage RNA-antigen complexes and their relevance to immune induction. J Exp Med. 1969 Sep 1;130(3):557–574. doi: 10.1084/jem.130.3.557. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unanue E. R., Askonas B. A. Persistence of immunogenicity of antigen after uptake by macrophages. J Exp Med. 1968 May 1;127(5):915–926. doi: 10.1084/jem.127.5.915. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unanue E. R., Askonas B. A. The immune response of mice to antigen in macrophages. Immunology. 1968 Aug;15(2):287–296. [PMC free article] [PubMed] [Google Scholar]
- Unanue E. R., Cerottini J. C., Bedford M. Persistence of antigen on the surface of macrophages. Nature. 1969 Jun 21;222(5199):1193–1195. doi: 10.1038/2221193a0. [DOI] [PubMed] [Google Scholar]
- Unanue E. R. The immune response of mice to keyhole limpet hemocyanin bound to macrophages. J Immunol. 1969 Apr;102(4):893–898. [PubMed] [Google Scholar]
- WEIGLE W. O. IMMUNOCHEMICAL PROPERTIES OF HEMOCYANIN. Immunochemistry. 1964 Dec;1:295–302. doi: 10.1016/0019-2791(64)90030-8. [DOI] [PubMed] [Google Scholar]