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. 1970 Feb 28;131(3):429–442. doi: 10.1084/jem.131.3.429

THE EFFECT OF GLUCOCORTICOSTEROIDS ON THE KINETICS OF MONONUCLEAR PHAGOCYTES

Jan Thompson 1, Ralph van Furth 1
PMCID: PMC2138814  PMID: 5413324

Abstract

The effect of glucocorticosteroids on the kinetics of mononuclear phagocytes, i.e., peripheral blood monocytes and peritoneal macrophages, was studied in normal mice, as well as in mice in which an inflammatory reaction was evoked in the peritoneal cavity. The administration of glucocorticosteroids resulted in a rapid decrease (within 3–6 hr) in the number of circulating monocytes, the duration being dependent on the nature and dose of the compound. The water-soluble dexamethasone sodium phosphate is only briefly active (less than 12 hr), but hydrocortisone acetate, which forms a subcutaneous depot, reduced the number of monocytes for more than 2 wk. In normal mice, hydrocortisone did not affect the number of macrophages already present in the peritoneal cavity, but the transit of mononuclear phagocytes from the circulation into the peritoneal cavity was arrested. During an inflammatory response in the peritoneal cavity, hydrocortisone suppresses both the increase in the number of monocytes in the peripheral blood and the increase in the number of peritoneal macrophages. This reduction of the inflammatory exudate appeared to be due to a diminished influx of mononuclear phagocytes from the peripheral blood. No lytic action of glucocorticosteroids on the mononuclear phagocytes could be demonstrated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALNER H. Identification of peritoneal macrophages in mouse radiation chimeras. Transplantation. 1963 Apr;1:217–223. doi: 10.1097/00007890-196301020-00009. [DOI] [PubMed] [Google Scholar]
  2. BOGGS D. R., ATHENS J. W., CARTWRIGHT G. E., WINTROBE M. M. THE EFFECT OF ADRENAL GLUCOCORTICOSTEROIDS UPON THE CELLULAR COMPOSITION OF INFLAMMATORY EXUDATES. Am J Pathol. 1964 May;44:763–773. [PMC free article] [PubMed] [Google Scholar]
  3. Boak J. L., Christie G. H., Ford W. L., Howard J. G. Pathways in the development of liver macrophages: alternative precursors contained in populations of lymphocytes and bone-marrow cells. Proc R Soc Lond B Biol Sci. 1968 Feb 27;169(1016):307–327. doi: 10.1098/rspb.1968.0013. [DOI] [PubMed] [Google Scholar]
  4. CUMMINGS M. M., DRUMMOND M. C., MICHAEL M., Jr, BLOOM W. L. The influence of cortisone on artificially induced peritoneal exudates. Bull Johns Hopkins Hosp. 1952 Mar;90(3):185–191. [PubMed] [Google Scholar]
  5. Craddock C. G., Winkelstein A., Matsuyuki Y., Lawrence J. S. The immune response to foreign red blood cells and the participation of short-lived lymphocytes. J Exp Med. 1967 Jun 1;125(6):1149–1172. doi: 10.1084/jem.125.6.1149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DOUGHERTY T. F., SCHNEEBELI G. L. Role of cortisone in regulation of inflammation. Proc Soc Exp Biol Med. 1950 Dec;75(3):854–859. doi: 10.3181/00379727-75-18368. [DOI] [PubMed] [Google Scholar]
  7. EBERT R. H., BARCLAY W. R. [Changes in connective tissue reaction induced by cortisone]. Ann Intern Med. 1952 Sep;37(3):506–518. doi: 10.7326/0003-4819-37-3-506. [DOI] [PubMed] [Google Scholar]
  8. Elliott E. V., Sinclair N. R. Effect of cortisone acetate on 19S and 7S haemolysin antibody. A time course study. Immunology. 1968 Nov;15(5):643–652. [PMC free article] [PubMed] [Google Scholar]
  9. FURNESS G. Effect of cortisone on the macrophages of different species of animal. J Bacteriol. 1959 Apr;77(4):461–464. doi: 10.1128/jb.77.4.461-464.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fauve R. M., Pierce-Chase C. H. Comparative effects of corticosteroids on host resistance to infection in relation to chemical structure. J Exp Med. 1967 May 1;125(5):807–821. doi: 10.1084/jem.125.5.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GELL P. G. H., HINDE I. T. The histology of the tuberculin reaction and its modification by cortisone. Br J Exp Pathol. 1951 Dec;32(6):516–529. [PMC free article] [PubMed] [Google Scholar]
  12. GERMUTH F. G., Jr, NEDZEL G. A., OTTINGER B., OYAMA J. Anatomic and histologic changes in rabbits with experimental hypersensitivity treated with Compound E and ACTH. Proc Soc Exp Biol Med. 1951 Jan;76(1):177–182. doi: 10.3181/00379727-76-18430. [DOI] [PubMed] [Google Scholar]
  13. GOODMAN J. W. ON THE ORIGIN OF PERITONEAL FLUID CELLS. Blood. 1964 Jan;23:18–26. [PubMed] [Google Scholar]
  14. Morse S. I. The effect of hydrocortisone and x-irradiation on the lymphocytosis induced by Bordetella pertussis. J Exp Med. 1966 Feb 1;123(2):283–297. doi: 10.1084/jem.123.2.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pinkett M. O., Cowdrey C. R., Nowell P. C. Mixed hematopoietic and pulmonary origin of 'alveolar macrophages' as demonstrated by chromosome markers. Am J Pathol. 1966 May;48(5):859–867. [PMC free article] [PubMed] [Google Scholar]
  16. SPEIRS R. S., WENCK U. Effect of cortisone on the cellular response during allergic inflammation. Acta Haematol. 1957 May;17(5):271–279. doi: 10.1159/000205247. [DOI] [PubMed] [Google Scholar]
  17. Spector W. G., Coote E. Differentially labelled blood cells in the reaction to paraffin oil. J Pathol Bacteriol. 1965 Oct;90(2):589–598. doi: 10.1002/path.1700900227. [DOI] [PubMed] [Google Scholar]
  18. Spector W. G., Walters M. N., Willoughby D. A. The origin of the mononuclear cells in inflammatory exudates induced by fibrinogen. J Pathol Bacteriol. 1965 Jul;90(1):181–192. doi: 10.1002/path.1700900119. [DOI] [PubMed] [Google Scholar]
  19. TOMPKINS E. H. The response of monocytes to adrenal cortical extract. J Lab Clin Med. 1952 Mar;39(3):365–371. [PubMed] [Google Scholar]
  20. VOLKMAN A., GOWANS J. L. THE ORIGIN OF MACROPHAGES FROM BONE MARROW IN THE RAT. Br J Exp Pathol. 1965 Feb;46:62–70. [PMC free article] [PubMed] [Google Scholar]
  21. VOLKMAN A., GOWANS J. L. THE PRODUCTION OF MACROPHAGES IN THE RAT. Br J Exp Pathol. 1965 Feb;46:50–61. [PMC free article] [PubMed] [Google Scholar]
  22. Virolainen M. Hematopoietic origin of macrophages as studied by chromosome markers in mice. J Exp Med. 1968 May 1;127(5):943–952. doi: 10.1084/jem.127.5.943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Volkman A. The origin and turnover of mononuclear cells in peritoneal exudates in rats. J Exp Med. 1966 Aug 1;124(2):241–254. doi: 10.1084/jem.124.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WENCK U., SPEIRS R. The effect of cortisone on blood leucocytes and peritoneal fluid cells of mice. Acta Haematol. 1957 Apr;17(4):193–201. doi: 10.1159/000205230. [DOI] [PubMed] [Google Scholar]
  25. van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]

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