Abstract
A number of azobenzenearsonate (ABA) conjugates have been prepared and tested for ability to react with antibody, to sensitize for hapten-specific delayed hypersensitivity and to induce hapten-specific unresponsiveness. All conjugates tested by in vitro or in vivo methods show a capacity to react with preformed antibody. Conjugates of L-amino acid polymers are immunogenic and induce tolerance. Conjugates of D-amino acid polymers or conjugates with high density of ABA groups are nonimmunogenic and fail to induce tolerance. Since paired D- and L-polymer conjugates react comparably with preformed antibody but differ markedly in tolerance induction, it is argued that combination with an antibody-like receptor molecule on the surface of an immune-competent cell is not a sufficient condition for tolerance. The lack of effectiveness of sterically crowded conjugates as well as D-polymer conjugates argues for a preliminary biologic "processing" of antigen necessary for induction of immunity or tolerance. Such a processing event might well involve enzymatic attack on the antigen.
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Selected References
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- Borek F., Stupp Y., Sela M. Formation and isolation of rabbit antibodies to a synthetic antigen of low molecular weight. J Immunol. 1967 Apr;98(4):739–744. [PubMed] [Google Scholar]
- Diener E., Armstrong W. D. Immunological tolerance in vitro: kinetic studies at the cellular level. J Exp Med. 1969 Mar 1;129(3):591–603. doi: 10.1084/jem.129.3.591. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dresser D. W., Mitchison N. A. The mechanism of immunological paralysis. Adv Immunol. 1968;8:129–181. doi: 10.1016/s0065-2776(08)60466-6. [DOI] [PubMed] [Google Scholar]
- Eskowitz S. Production of hapten-specific unresponsiveness in adult guinea-pigs by prior injection of monovalent conjugates. Immunology. 1967 Jul;13(1):9–17. [PMC free article] [PubMed] [Google Scholar]
- GELL P. G., BENACERRAF B. Studies on hypersensitivity. IV. The relationship between contact and delayed sensitivity: a study of the specificity of cellular immune reactions. J Exp Med. 1961 Mar 1;113:571–585. doi: 10.1084/jem.113.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gill T. J., 3rd, Papermaster D. S., Mowbray J. F. Synthetic polypeptide metabolism. I. The metabolic fate of enantiomorphic polymers. J Immunol. 1965 Nov;95(5):794–803. [PubMed] [Google Scholar]
- Leskowitz S., Jones V. E., Zak S. J. Immunochemical study of antigenic specificity in delayed hypersensitivity. V. Immunization with monovalent low molecular weight conjugates. J Exp Med. 1966 Feb 1;123(2):229–237. doi: 10.1084/jem.123.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leskowitz S. Mechanism of delayed reactions. Science. 1967 Jan 20;155(3760):350–352. doi: 10.1126/science.155.3760.350. [DOI] [PubMed] [Google Scholar]
- Leskowitz S. Tolerance. Annu Rev Microbiol. 1967;21:157–180. doi: 10.1146/annurev.mi.21.100167.001105. [DOI] [PubMed] [Google Scholar]
- OVARY Z., KARUSH F. Studies on the immunologic mechanism of anaphylaxis. I. Antibody-hapten interactions studied by passive cutaneous anaphylaxis in the guinea pig. J Immunol. 1960 Apr;84:409–415. [PubMed] [Google Scholar]
- Schlossman S. F., Ben-Efraim S., Yaron A., Sober H. A. Immunochemical studies on the antigenic determinants required to elicit delayed and immediate hypersensitivity reactions. J Exp Med. 1966 Jun 1;123(6):1083–1095. doi: 10.1084/jem.123.6.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TABACHNICK M., SOBOTKA H. Azoproteins. I. Spectrophotometric studies of amino acid azo derivatives. J Biol Chem. 1959 Jul;234(7):1726–1730. [PubMed] [Google Scholar]
- WASSERMAN E., LEVINE L. Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition. J Immunol. 1961 Sep;87:290–295. [PubMed] [Google Scholar]
- WOFSY L., METZGER H., SINGER S. J. Affinity labeling-a general method for labeling the active sites of antibody and enzyme molecules. Biochemistry. 1962 Nov;1:1031–1039. doi: 10.1021/bi00912a013. [DOI] [PubMed] [Google Scholar]