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. 1987 Nov;169(11):4901–4906. doi: 10.1128/jb.169.11.4901-4906.1987

LiCl treatment releases a nickase implicated in genetic transformation of Streptococcus pneumoniae.

T Fujii 1, D Naka 1, N Toyoda 1, H Seto 1
PMCID: PMC213883  PMID: 2822653

Abstract

When cells competent for genetic transformation of Streptococcus pneumoniae which could bind and enable entry of extracellular DNA molecules were treated with LiCl, they released a nickase that introduced nicks into a double-stranded DNA in the presence of EDTA. The nickase was specific for competent cells and coupled with DNA-binding activity. Furthermore, when noncompetent cells were treated with LiCl, they released the putative receptors for the competence activator.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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