Skip to main content
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1970 Jun 1;131(6):1342–1357. doi: 10.1084/jem.131.6.1342

STUDIES ON THE ULTRASTRUCTURE OF BORDETELLA PERTUSSIS

I. MORPHOLOGY, ORIGIN, AND BIOLOGICAL ACTIVITY OF STRUCTURES PRESENT IN THE EXTRACELLUAR FLUID OF LIQUID CULTURES OF BORDETELLA PERTUSSIS

Jane H Morse 1, Stephen I Morse 1
PMCID: PMC2138840  PMID: 4315895

Abstract

Two distinct particles have been recognized in the extracellular fluid of B. pertussis cultures. Both appeared to arise from the surface (cell wall) of the organism. One of these, a membranous particle, seemed to derive from long projections on the organism composed of the outer membrane of the cell wall. The second particle, a fine filament, was not readily comparable with any previously described bacterial structure. The two particles could be separated from each other by gradient centrifugation in CsCl. Lymphocytosis-promoting factor and histamine-sensitizing activity were only associated with fractions containing the fine filaments.

Full Text

The Full Text of this article is available as a PDF (1.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLADEN H. A., MERGENHAGEN S. E. ULTRASTRUCTURE OF VEILLONELLA AND MORPHOLOGICAL CORRELATION OF AN OUTER MEMBRANE WITH PARTICLES ASSOCIATED WITH ENDOTOXIC ACTIVITY. J Bacteriol. 1964 Nov;88:1482–1492. doi: 10.1128/jb.88.5.1482-1492.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bayer M. E., Anderson T. F. The surface structure of Escherichia coli. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1592–1599. doi: 10.1073/pnas.54.6.1592. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Imaeda T., Kanetsuna F., Galindo B. Ultrastructure of cell walls of genus Mycobacterium. J Ultrastruct Res. 1968 Oct;25(1):46–63. doi: 10.1016/s0022-5320(68)80059-0. [DOI] [PubMed] [Google Scholar]
  4. Imaeda T., Kanetsuna F., Rieber M., Galindo B., Cesari I. M. Ultrastructural characteristics of mycobacterial growth. J Med Microbiol. 1969 Aug;2(3):181–186. doi: 10.1099/00222615-2-3-181. [DOI] [PubMed] [Google Scholar]
  5. Kuo T. T., Huang T. C., Chow T. Y. A filamentous bacteriophage from Xanthomonas oryzae. Virology. 1969 Nov;39(3):548–555. doi: 10.1016/0042-6822(69)90102-0. [DOI] [PubMed] [Google Scholar]
  6. Lane A. G. Appearance of mouse-lethal toxin in liquid cultures of Bordetella pertussis. Appl Microbiol. 1968 Sep;16(9):1400–1405. doi: 10.1128/am.16.9.1400-1405.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mason M. A. The spheroplasts of bordetella pertussis. Can J Microbiol. 1966 Jun;12(3):539–545. doi: 10.1139/m66-077. [DOI] [PubMed] [Google Scholar]
  8. Milleck J., Rockstroh T., Ocklitz H. W. Die Feinstruktur glycininduzierter Sphäroplasten im Vergleich zu der normaler Bakterienzellen von Bordetella pertussis. Zentralbl Bakteriol Orig. 1968;209(1):37–43. [PubMed] [Google Scholar]
  9. Morse S. I., Bray K. K. The occurrence and properties of leukocytosis and lymphocytosis-stimulating material in the supernatant fluids of Bordetella pertussis cultures. J Exp Med. 1969 Mar 1;129(3):523–550. doi: 10.1084/jem.129.3.523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Munoz J., Bergman R. K. Histamine-sensitizing factors from microbial agents, with special reference to Bordetella pertussis. Bacteriol Rev. 1968 Jun;32(2):103–126. doi: 10.1128/br.32.2.103-126.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pieroni R. E., Broderick E. J., Levine L. The soluble protective antigen and the histamine-sensitizing factor of Bordetella pertussis. J Immunol. 1965 Oct;95(4):643–650. [PubMed] [Google Scholar]
  12. Work E., Knox K. W., Vesk M. The chemistry and electron microscopy of an extracellular lipopolysaccharide from Escherichia coli. Ann N Y Acad Sci. 1966 Jun 30;133(2):438–449. doi: 10.1111/j.1749-6632.1966.tb52382.x. [DOI] [PubMed] [Google Scholar]
  13. ZINDER N. D., VALENTINE R. C., ROGER M., STOECKENIUS W. F1, A ROD-SHAPED MALE-SPECIFIC BACTERIOPHAGE THAT CONTAINS DNA. Virology. 1963 Aug;20:638–640. doi: 10.1016/0042-6822(63)90290-3. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES