Skip to main content
NCBI home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1970 Jun 1;131(6):1169–1189. doi: 10.1084/jem.131.6.1169

THE EMERGENCE OF ANTIBODIES WITH EITHER IDENTICAL OR UNRELATED INDIVIDUAL ANTIGENIC SPECIFICITY DURING REPEATED IMMUNIZATIONS WITH STREPTOCOCCAL VACCINES

Klaus Eichmann 1, Dietmar G Braun 1, Ten Feizi 1, Richard M Krause 1
PMCID: PMC2138849  PMID: 4192569

Abstract

Electrophoretically monodisperse antibody components in rabbit antisera to the carbohydrates of the Groups A and C streptococci have been examined for their individual antigenic specificity. In these antibody components which were isolated by preparative electrophoresis, individual antigenic specificity was confined to the specific antibody and was absent in the nonantibody γ-globulin. Radioprecipitation experiments and the use of immune absorbent columns constructed from goat anti-antisera, which had been absorbed with fraction II, revealed that all the specific antibody in an electrophoretically monodisperse component was reactive with the homologous anti-antibody. Antibodies with either identical or distinct individual antigenic specificities may occur in the same rabbit with repeated immunizations. Antibodies with identical antigenic specificity had identical electrophoretic mobility, whereas antibodies with unrelated antigenic specificities had distinct electrophoretic mobilities. In the interval between immunizations, if antibody to the carbohydrate antigen was absent, there was no detectable antibody with individual antigenic specificity.

Full Text

The Full Text of this article is available as a PDF (1.1 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALLLEN J. C., KUNKEL H. G., KABAT E. A. STUDIES ON HUMAN ANTIBODIES. II. DISTRIBUTION OF GENETIC FACTORS. J Exp Med. 1964 Mar 1;119:453–465. doi: 10.1084/jem.119.3.453. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Avrameas S., Ternynck T. The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969 Jan;6(1):53–66. doi: 10.1016/0019-2791(69)90178-5. [DOI] [PubMed] [Google Scholar]
  3. BLEIWEIS A. S., KARAKAWA W. W., KRAUSE R. M. IMPROVED TECHNIQUE FOR THE PREPARATION OF STREPTOCOCCAL CELL WALLS. J Bacteriol. 1964 Oct;88:1198–1200. doi: 10.1128/jb.88.4.1198-1200.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Braun D. G., Eichmann K., Krause R. M. Rabbit antibodies to streptococcal carbohydrates. Influence of primary and secondary immunization and of possible genetic factors on the antibody response. J Exp Med. 1969 Apr 1;129(4):809–830. doi: 10.1084/jem.129.4.809. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Braun D. G., Krause R. M. The individual antigenic specificity of antibodies to streptococcal carbohydrates. J Exp Med. 1968 Nov 1;128(5):969–989. doi: 10.1084/jem.128.5.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Daugharty H., Hopper J. E., MacDonald A. B., Nisonoff A. Quantitative investigations of idiotypic antibodies. I. Analysis of precipitating antibody populations. J Exp Med. 1969 Nov 1;130(5):1047–1062. doi: 10.1084/jem.130.5.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Eichmann K., Lackland H., Hood L., Krause R. M. Induction of rabbit antibody with molecular uniformity after immunization with group C streptococci. J Exp Med. 1970 Jan 1;131(1):207–221. doi: 10.1084/jem.131.1.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fleischman J. B., Braun D. G., Krause R. M. Streptococcal group-specific antibodies: occurrence of a restricted population following secondary immunization. Proc Natl Acad Sci U S A. 1968 May;60(1):134–139. doi: 10.1073/pnas.60.1.134. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GREY H. M., MANNIK M., KUNKEL H. G. INDIVIDUAL ANTIGENIC SPECIFICITY OF MYELOMA PROTEINS. CHARACTERISTICS AND LOCALIZATION TO SUBUNITS. J Exp Med. 1965 Apr 1;121:561–575. doi: 10.1084/jem.121.4.561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HOLM S. E. A MODIFIED GEL CHAMBER TECHNIQUE FOR IMMUNODIFFUSION ANALYSIS. Int Arch Allergy Appl Immunol. 1965;26:34–43. doi: 10.1159/000229544. [DOI] [PubMed] [Google Scholar]
  11. Hood L., Lackland H., Eichman K., Kindt T. J., Braun D. G., Krause R. M. Amino acid sequence restriction in rabbit antibody light chains. Proc Natl Acad Sci U S A. 1969 Jul;63(3):890–896. doi: 10.1073/pnas.63.3.890. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KRAUSE R. M., McCARTY M. Studies on the chemical structure of the streptococcal cell wall. II. The composition of group C cell walls and chemical basis for serologic specificity of the carbohydrate moiety. J Exp Med. 1962 Jan 1;115:49–62. doi: 10.1084/jem.115.1.49. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kelus A. S., Gell P. G. Immunological analysis of rabbit anti-antibody systems. J Exp Med. 1968 Jan 1;127(1):215–234. doi: 10.1084/jem.127.1.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kindt T. J., Todd C. W., Eichmann K., Krause R. M. Allotype exclusion in uniform rabbit antibody to streptococcal carbohydrate. J Exp Med. 1970 Feb;131(2):343–354. doi: 10.1084/jem.131.2.343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kunkel H. G. Experimental approaches tohomogenous antibody populations. iIndividual antigenic specificity, cross specificity and diversity of human antibodies. Fed Proc. 1970 Jan-Feb;29(1):55–58. [PubMed] [Google Scholar]
  16. Kunkel H. G., Mannik M., Williams R. C. Individual Antigenic Specificity of Isolated Antibodies. Science. 1963 Jun 14;140(3572):1218–1219. doi: 10.1126/science.140.3572.1218. [DOI] [PubMed] [Google Scholar]
  17. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  18. MCCARTY M. Variation in the group-specific carbohydrate of group A streptococci. II. Studies on the chemical basis for serological specificity of the carbohydrates. J Exp Med. 1956 Nov 1;104(5):629–643. doi: 10.1084/jem.104.5.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MacDonald A. B., Alescio L., Nisonoff A. Biosynthesis of antibody molecules with similar properties during prolonged immunization. Biochemistry. 1969 Jul;8(7):3109–3114. doi: 10.1021/bi00835a062. [DOI] [PubMed] [Google Scholar]
  20. MacDonald A. B., Nisonoff A. Quantitative investigations of idiotypic antibodies. 3. Persistence and variations of idiotypic specificities during the course of immunization. J Exp Med. 1970 Mar 1;131(3):583–601. doi: 10.1084/jem.131.3.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. McCARTY M. Further studies on the chemical basis for serological specificity of Group A streptococcal carbohydrate. J Exp Med. 1958 Sep 1;108(3):311–323. doi: 10.1084/jem.108.3.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Miller E. J., Osterland C. K., Davie J. M., Krause R. M. Electrophoretic analysis of polypeptide chains isolated from antibodies in the serum of immunized rabbits. J Immunol. 1967 Apr;98(4):710–715. [PubMed] [Google Scholar]
  23. NOSSAL G. J., MAKELA O. Autoradiographic studies on the immune response.I. The kinetics of plasma cell proliferation. J Exp Med. 1962 Jan 1;115:209–230. doi: 10.1084/jem.115.1.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Osterland C. K., Miller E. J., Karakawa W. W., Krause R. M. Characteristics of streptococcal group-specific antibody isolated from hyperimmune rabbits. J Exp Med. 1966 Apr 1;123(4):599–614. doi: 10.1084/jem.123.4.599. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Oudin J., Michel M. Idiotypy of rabbit antibodies. I. Comparison of idiotypy of antibodies against Salmonella typhi with that of antibodies against other bacteria in the same rabbits, or of antibodies against Salmonella typhi in various rabbits. J Exp Med. 1969 Sep 1;130(3):595–617. doi: 10.1084/jem.130.3.595. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Oudin J., Michel M. Idiotypy of rabbit antibodies. II. Comparison of idiotypy of various kinds of antibodies formed in the same rabbits against Salmonella typhi. J Exp Med. 1969 Sep 1;130(3):619–642. doi: 10.1084/jem.130.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Reisfeld R. A., Small P. A., Jr Electrophoretic heterogeneity of polypeptide chains of specific antibodies. Science. 1966 May 27;152(3726):1253–1255. doi: 10.1126/science.152.3726.1253. [DOI] [PubMed] [Google Scholar]
  28. SCHOOLEY J. C. Autoradiographic observations of plasma cell formation. J Immunol. 1961 Mar;86:331–337. [PubMed] [Google Scholar]
  29. Yount W. J., Dorner M. M., Kunkel H. G., Kabat E. A. Studies on human antibodies. VI. Selective variations in subgroup composition and genetic markers. J Exp Med. 1968 Mar 1;127(3):633–646. doi: 10.1084/jem.127.3.633. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES