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. 1970 Oct 31;132(5):941–950. doi: 10.1084/jem.132.5.941

COMPLEMENT AND COMPLEMENT-LIKE ACTIVITY IN LOWER VERTEBRATES AND INVERTEBRATES

N K B Day 1, H Gewurz 1, R Johannsen 1, J Finstad 1, R A Good 1
PMCID: PMC2138876  PMID: 5470510

Abstract

A purified cobra venom factor with C-inhibiting activity also promotes lysis of erythrocytes in fresh mammalian serum. Lysis-inducing activity of purified cobra venom factor was found in sera of lower vertebrates including the cyclostome hagfish and in invertebrates. Lysis-inducing activity was most effective with frog serum. Frog serum was found to be more hemolytic for Es in the presence of CVF than when cells were sensitized with hemolysin. The hemolysis induced by CVF with frog serum, as in the higher vertebrates, was inhibited when sera were pretreated with known C inhibitors including heat, chelators, endotoxin, immune complexes, and CVF itself. Complexes formed with CVF and either frog serum or invertebrate hemolymph promoted lysis of indicator cells in the presence of frog serum in EDTA. This lysis was most marked when the starfish-CVF complex was used and was C-dependent. Conversely, complex formed with frog serum and CVF promoted lysis of E in the presence of invertebrate hemolymph (Limulus) in EDTA. Hence, serum components were to some degree at least interchangeable between vertebrate sera and invertebrate hemolymph. Lysis-inducing activity of purified CVF occurs in a wide range of species, has revealed activities resembling those of terminal C-components in lower vertebrates and invertebrates, and provides one means for study of C and C-like activities in primitive species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ballow M., Cochrane C. G. Two anticomplementary factors in cobra venom: hemolysis of guinea pig erythrocytes by one of them. J Immunol. 1969 Nov;103(5):944–952. [PubMed] [Google Scholar]
  2. Cochrane C. G. Immunologic tissue injury mediated by neutrophilic leukocytes. Adv Immunol. 1968;9:97–162. doi: 10.1016/s0065-2776(08)60442-3. [DOI] [PubMed] [Google Scholar]
  3. Cooper E. L. Chronic allograft rejection in Lumbricus terrestris. J Exp Zool. 1969 May;171(1):69–74. doi: 10.1002/jez.1401710110. [DOI] [PubMed] [Google Scholar]
  4. Flexner S., Noguchi H. SNAKE VENOM IN RELATION TO HAEMOLYSIS, BACTERIOLYSIS, AND TOXICITY. J Exp Med. 1902 Mar 1;6(3):277–301. doi: 10.1084/jem.6.3.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GOOD R. A., PAPERMASTER B. W. ONTOGENY AND PHYLOGENY OF ADAPTIVE IMMUNITY. Adv Immunol. 1964;27:1–115. doi: 10.1016/s0065-2776(08)60706-3. [DOI] [PubMed] [Google Scholar]
  6. Gewurz H., Mergenhagen S. E., Nowotny A., Phillips J. K. Interactions of the complement system with native and chemically modified endotoxins. J Bacteriol. 1968 Feb;95(2):397–405. doi: 10.1128/jb.95.2.397-405.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Legler D. W., Evans E. E. Comparative immunology: hemolytic complement in elasmobranchs. Proc Soc Exp Biol Med. 1967 Jan;124(1):30–34. doi: 10.3181/00379727-124-31659. [DOI] [PubMed] [Google Scholar]
  8. Mergenhagen S. E., Snyderman R., Gewurz H., Shin H. S. Significance of complement to the mechanism of action of endotoxin. Curr Top Microbiol Immunol. 1969;50:37–77. doi: 10.1007/978-3-642-46169-9_2. [DOI] [PubMed] [Google Scholar]
  9. Nelson R. A., Jr A new concept of immunosuppression in hypersensitivity reactions and in transplantation immunity. Surv Ophthalmol. 1966 Aug;11(4):498–505. [PubMed] [Google Scholar]
  10. Nelson R. A., Jr, Jensen J., Gigli I., Tamura N. Methods for the separation, purification and measurement of nine components of hemolytic complement in guinea-pig serum. Immunochemistry. 1966 Mar;3(2):111–135. doi: 10.1016/0019-2791(66)90292-8. [DOI] [PubMed] [Google Scholar]
  11. Pickering R. J., Wolfson M. R., Good R. A., Gewurz H. Passive hemolysis by serum and cobra venom factor: a new mechanism inducing membrane damage by complement. Proc Natl Acad Sci U S A. 1969 Feb;62(2):521–527. doi: 10.1073/pnas.62.2.521. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shin H. S., Gewurz H., Snyderman R. Reaction of a cobra venom factor with guinea pig complement and generation of an activity chemotactic for polymorphonuclear leukocytes. Proc Soc Exp Biol Med. 1969 May;131(1):203–207. doi: 10.3181/00379727-131-33840. [DOI] [PubMed] [Google Scholar]
  13. Ward P. A. A plasmin-split fragment of C'3 as a new chemotactic factor. J Exp Med. 1967 Aug 1;126(2):189–206. doi: 10.1084/jem.126.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Willoughby D. A., Coote E., Turk J. L. Complement in acute inflammation. J Pathol. 1969 Feb;97(2):295–305. doi: 10.1002/path.1710970215. [DOI] [PubMed] [Google Scholar]

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