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The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1971 Jan 1;133(1):156–167. doi: 10.1084/jem.133.1.156

IMMUNOGLOBULINS ON THE SURFACE OF LYMPHOCYTES

I. DISTRIBUTION AND QUANTITATION

Enrique Rabellino 1, Sonia Colon 1, Howard M Grey 1, Emil R Unanue 1
PMCID: PMC2138891  PMID: 4099713

Abstract

The distribution, and quantity of immunoglobulins on the surface of lymphocytes has been studied by means of immunofluorescence and a quantitative radio-immunoassay. Surface immunoglobulins were found on approximately 45% of spleen and marrow lymphocytes and 7–14% of lymphocytes from lymph nodes, peripheral blood, and peritoneal exudate. Thymic lymphocytes contained undetectable amounts of immunoglobulin. In the spleen the different immunoglobulins were present in the following order: γG2 > γG1 > M > γA > γG3. The surface immunoglobulin was largely removable by brief treatment with trypsin. Quantitative analysis indicated that 50,000–150,000 molecules of immunoglobulin were present on an individual cell. A variety of observations make it likely that this lymphocyte-associated immunoglobulin. is a product of the cell to which it is attached rather than a form of cytophilic antibody.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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