Abstract
A study of the kinetics of antibody-producing cells has been carried out by the use of rosette formation for detection of individual antibody-producing cells, and labeling with tritiated thymidine, in cells obtained from mouse spleens at intervals after injection of SRBC. Following a primary injection of the antigen, the number of RFC per million cells was found to increase to a peak at 5 days, then, after a decrease, to a second peak at about the 10th day. The curve of tritium labeling of RFC was also biphasic, with peaks on the 3rd and 7th day. The second increase in rosette-forming cells could be shown to involve, especially between the 7th and 9th day, a second increase in lymphoid cell RFC and, among these, 7S antibody-producing cells. When the population examined was restricted to large lymphocytes, two peaks of RFC per million cells and two peaks of labeling were again found. In this case, however, the peaks of RFC and of labeling were reached on the same day in each instance, rather than with the 2 day difference found in the entire spleen cell suspension or the entire lymphoid cell population. Electron microscopic examination of labeled rosette-forming cells showed these to be largely lymphocytes, but to include rather well differentiated plasmablasts as well. No macrophages were found among labeled RFC in the primary response. A substantial number of labeled lymphocytes were found in close contiguity with rosette-forming macrophages. The percentage of labeling in such lymphocytes was as high, on the respective days, as the percentage of labeled cells among the RFC of the entire suspension.
Full Text
The Full Text of this article is available as a PDF (964.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Avrameas S., Leduc E. H. Detection of simultaneous antibody synthesis in plasma cells and specialized lymphocytes in rabbit lymph nodes. J Exp Med. 1970 Jun 1;131(6):1137–1168. doi: 10.1084/jem.131.6.1137. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CARO L. G., VAN TUBERGEN R. P., KOLB J. A. High-resolution autoradiography. I. Methods. J Cell Biol. 1962 Nov;15:173–188. doi: 10.1083/jcb.15.2.173. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cunningham A. J., Smith J. B., Mercer E. H. Antibody formation by single cells from lymph nodes and efferent lymph of sheep. J Exp Med. 1966 Oct 1;124(4):701–714. doi: 10.1084/jem.124.4.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dutton R. W., Mishell R. I. Cell populations and cell proliferation in the in vitro response of normal mouse spleen to heterologous erythrocytes. Analysis by the hot pulse technique. J Exp Med. 1967 Sep 1;126(3):443–454. doi: 10.1084/jem.126.3.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gudat F. G., Harris T. N., Harris S., Hummeler K. Studies on antibody-producing cells. I. Ultrastructure of 19S and 7S antibody-producing cells. J Exp Med. 1970 Sep 1;132(3):448–474. doi: 10.1084/jem.132.3.448. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harris T. N., Hummeler K., Harris S. Electron microscopic observations on antibody-producing lymph node cells. J Exp Med. 1966 Jan 1;123(1):161–172. doi: 10.1084/jem.123.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hummeler K., Harris T. N., Tomassini N., Hechtel M., Farber M. B. Electron microscopic observations on antibody-producing cells in lymph and blood. J Exp Med. 1966 Aug 1;124(2):255–262. doi: 10.1084/jem.124.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koros A. M., Mazur J. M., Mowery M. J. Radioautographic studies of plaque-forming cells. I. Antigen-stimulated proliferation of plaque-forming cells. J Exp Med. 1968 Aug 1;128(2):235–257. doi: 10.1084/jem.128.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mosier D. E. A requirement for two cell types for antibody formation in vitro. Science. 1967 Dec 22;158(3808):1573–1575. doi: 10.1126/science.158.3808.1573. [DOI] [PubMed] [Google Scholar]
- NOSSAL G. J., SZENBERG A., ADA G. L., AUSTIN C. M. SINGLE CELL STUDIES ON 19S ANTIBODY PRODUCTION. J Exp Med. 1964 Mar 1;119:485–502. doi: 10.1084/jem.119.3.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Storb U., Bauer W., Storb R., Fliedner T. M., Weiser R. S. Ultrastructure of rosette-forming cells in the mouse during the antibody response. J Immunol. 1969 Jun;102(6):1474–1485. [PubMed] [Google Scholar]
- Storb U., Chambers V., Storb R., Weiser R. S. Antibody-carrying cells in the immune response II. Ultrastructure of "rosette"-forming cells. J Reticuloendothel Soc. 1967 Jan;4(1):69–83. [PubMed] [Google Scholar]
- Storb U., Weiser R. S. Antibody-carrying cells in the immune response 1. Identification of "rosette"-forming cells by light microscopy. J Reticuloendothel Soc. 1967 Jan;4(1):51–68. [PubMed] [Google Scholar]
- Storb U., Weiser R. S. Kinetics of mouse spleen cell populations during the immune response. J Reticuloendothel Soc. 1968 Apr;5(2):81–106. [PubMed] [Google Scholar]
- TRUMP B. F., SMUCKLER E. A., BENDITT E. P. A method for staining epoxy sections for light microscopy. J Ultrastruct Res. 1961 Aug;5:343–348. doi: 10.1016/s0022-5320(61)80011-7. [DOI] [PubMed] [Google Scholar]
- URSO P., MAKINODAN T. THE ROLES OF CELLULAR DIVISION AND MATURATION IN THE FORMATION OF PRECIPITATING ANTIBODY. J Immunol. 1963 Jun;90:897–907. [PubMed] [Google Scholar]