Abstract
An enzyme with the characteristics of classical renin was isolated from brain extracts of nephrectomized dogs. This enzyme is thermolabile, nondialyzable, and forms a vasoconstrictive material when incubated with renin plasma substrate at pH 7. A short lasting pressor activity was also found in brain extracts of dogs and rats. This activity was due to a substance identified by chemical and pharmacological tests as angiotensin. Countercurrent distribution of brain extracts of rats showed that 38% of the pressor activity corresponded to angiotensin II and the remainder to angiotensin I. A remarkable correlation was found between angiotensin and norepinphrine concentrations in different portions of the encephalon of the dog.
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Selected References
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- Barrett J. D., Sambhi M. P. Simultaneous assay of angiotensin I and II and determination of converting enzyme activity. J Pharmacol Exp Ther. 1969 Dec;170(2):326–333. [PubMed] [Google Scholar]
- Boadle M. C., Hughes J., Roth R. H. Angiotensin accelerates catecholamine biosynthesis in sympathetically innervated tissues. Nature. 1969 Jun 7;222(5197):987–988. doi: 10.1038/222987a0. [DOI] [PubMed] [Google Scholar]
- CARLSSON A. The occurrence, distribution and physiological role of catecholamines in the nervous system. Pharmacol Rev. 1959 Jun;11(2 Pt 2):490–493. [PubMed] [Google Scholar]
- DAVIS J. O. The control of aldosterone secretion. Physiologist. 1962 May;5:65–86. [PubMed] [Google Scholar]
- Finkielman S., Nahmod V. E. In vitro production of angiotensin I by renal glomeruli. Nature. 1969 Jun 21;222(5199):1186–1188. doi: 10.1038/2221186a0. [DOI] [PubMed] [Google Scholar]
- GOULD A. B., SKEGGS L. T., Jr, KAHN J. R. THE PRESENCE OF RENIN ACTIVITY IN BLOOD VESSEL WALLS. J Exp Med. 1964 Mar 1;119:389–399. doi: 10.1084/jem.119.3.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haas E., Goldblatt H., Gipson E. C., Lewis L. Extraction, purification, and assay of human renin free of angiotensinase. Circ Res. 1966 Oct;19(4):739–749. doi: 10.1161/01.res.19.4.739. [DOI] [PubMed] [Google Scholar]
- Hayduk K., Boucher R., Genest J. Renin activity content in various tissues od dogs under different physiopathological states. Proc Soc Exp Biol Med. 1970 May;134(1):252–255. doi: 10.3181/00379727-134-34770. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- McCubbin J. W., DeMoura R. S., Page I. H., Olmsted F. Arterial hypertension elicited by subpressor amounts of angiotensin. Science. 1965 Sep 17;149(3690):1394–1395. doi: 10.1126/science.149.3690.1394. [DOI] [PubMed] [Google Scholar]
- PEART W. S. THE RENIN-ANGIOTENSIN SYSTEM. Pharmacol Rev. 1965 Jun;17:143–182. [PubMed] [Google Scholar]
- Peach M. J., Bumpus F. M., Khairallah P. A. Inhibition of norepinephrine uptake in hearts by angiotensin II and analogs. J Pharmacol Exp Ther. 1969 Jun;167(2):291–299. [PubMed] [Google Scholar]
- Roth M., Weitzman A. F., Piquilloud Y. Converting enzyme content of different tissues of the rat. Experientia. 1969 Dec 15;25(12):1247–1247. doi: 10.1007/BF01897474. [DOI] [PubMed] [Google Scholar]
- SCORNIK O. A., PALADINI A. C. Angiotension blood levels in dogs with experimental renal hypertension. Am J Physiol. 1961 Sep;201:526–530. doi: 10.1152/ajplegacy.1961.201.3.526. [DOI] [PubMed] [Google Scholar]
- Skeggs L. T., Lentz K. E., Kahn J. R., Dorer F. E., Levine M. Pseudorenin. A new angiotensin-forming enzyme. Circ Res. 1969 Oct;25(4):451–462. doi: 10.1161/01.res.25.4.451. [DOI] [PubMed] [Google Scholar]
- Smookler H. H., Severs W. B., Kinnard W. J., Buckley J. P. Centrally mediated cardiovascular effects of angiotensin II. J Pharmacol Exp Ther. 1966 Sep;153(3):485–494. [PubMed] [Google Scholar]