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. 1971 May 1;133(5):1061–1073. doi: 10.1084/jem.133.5.1061

A CELLULAR DEFICIT IN THE RECONSTITUTIVE CAPACITY OF IMMUNE POPULATIONS OF LYMPHOID CELLS DEMONSTRABLE IN STUDIES OF DELAYED HYPERSENSITIVITY IN MICE

EVIDENCE FOR THYMUS-BONE MARROW CELL SYNERGISM

David Eidinger 1, Aleksander Ackerman 1
PMCID: PMC2138914  PMID: 4928817

Abstract

A cell-transfer system was employed in the present work to investigate several characteristics of the capacity of immune and normal lymphoid cells to transfer the delayed response to methylated human serum albumin in lethally irradiated syngeneic recipients. Spleen cells derived from donor mice immunized with goose erythrocytes were far less effective in transferring responsiveness when compared with equal numbers of normal cells. Statistical analyses indicated a frequency of 1 reactive cell or cell unit in 1.3 x 107 normal cells and in 6.2 x 107 immune cells. These findings provided confirmatory evidence that antigen-induced suppression (antigenic competition) employing sequential administration of two non-cross-reacting antigens is due to relative deficits of immunocompetent cells generated by lymphoproliferation in lymphoid tissues secondary to immunization with the initial antigen. The cellular deficit in the immune population was shown to be resident in a thymus cell population, which restored the number of responders to a level equivalent to the normal population. The thymic cell was akin to the antigen-reactive cell. The cell limiting the degree of response, that is the effector cell for both normal and immune cell populations, was of bone marrow origin. Both populations of cells were shown to act in synergy to reconstitute the delayed response to the antigen.

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Selected References

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  1. Cantor H., Asofsky R. Synergy among lymphoid cells mediating the graft-versus-host response. II. Synergy in graft-versus-host reactions produced by Balb-c lymphoid cells of differing anatomic origin. J Exp Med. 1970 Feb;131(2):235–246. doi: 10.1084/jem.131.2.235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Claman H. N., Chaperon E. A., Triplett R. F. Thymus-marrow cell combinations. Synergism in antibody production. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1167–1171. doi: 10.3181/00379727-122-31353. [DOI] [PubMed] [Google Scholar]
  3. Coe J. E., Feldman J. D., Lee S. Immunologic competence of thoracic duct cells. I. Delayed hypersensitivity. J Exp Med. 1966 Feb 1;123(2):267–281. doi: 10.1084/jem.123.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crowle A. J., Patrucco A. Preferential development by mice of delayed hypersensitivity to purified basic proteins. J Allergy. 1968 Sep;42(3):140–156. doi: 10.1016/0021-8707(68)90087-7. [DOI] [PubMed] [Google Scholar]
  5. Eidinger D., Khan S. A., Millar K. G. The effect of antigenic competition on various manifestations of humoral antibody formation and cellular immunity. J Exp Med. 1968 Nov 1;128(5):1183–1200. doi: 10.1084/jem.128.5.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Elkins W. L. The interaction of donor and host lymphoid cells in the pathogenesis of renal cortical destruction induced by a local graft versus host reaction. J Exp Med. 1966 Jan 1;123(1):103–118. doi: 10.1084/jem.123.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Green I., Paul W. E., Benacerraf B. A study of the passive transfer of delayed hypersensitivity to DNP-poly-L-lysine and DNP-GL in responder and nonresponder guinea pigs. J Exp Med. 1967 Nov 1;126(5):959–967. doi: 10.1084/jem.126.5.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Groves D. L., Lever W. E., Makinodan T. A model for the interaction of cell types in the generation of hemolytic plaque-forming cells. J Immunol. 1970 Jan;104(1):148–165. [PubMed] [Google Scholar]
  9. Halliday W. J., Webb M. Delayed hypersensitivity to chemically induced tumors in mice and correlation with an in vitro test. J Natl Cancer Inst. 1969 Jul;43(1):141–150. [PubMed] [Google Scholar]
  10. Hilgard H. R. Synergism of thymus and bone marrow in the production of gra a5hilgard HR: Synergism of thymus and bone marrow in the production of graft-versus-host splenomegaly in x-irradiated hosts. J Exp Med. 1970 Aug 1;132(2):317–328. doi: 10.1084/jem.132.2.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kerbel R. S., Eidinger D. Further studies of antigenic competition. 3. A model to account for the phenomenon based on a deficiency of cell-to-cell interaction in immune lymphoid cell populations. J Exp Med. 1971 May 1;133(5):1043–1060. doi: 10.1084/jem.133.5.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. LIACOPOULOS P., NEVEU T. NON-SPECIFIC INHIBITION OF THE IMMEDIATE AND DELAYED TYPES OF HYPERSENSITIVITY DURING IMMUNE PARALYSIS OF ADULT GUINEA-PIGS. Immunology. 1964 Jan;7:26–39. [PMC free article] [PubMed] [Google Scholar]
  13. Lawrence W., Jr, Simonsen M. The property of "strength" of histocompatibility antigens, and their ability to produce antigenic competition. Transplantation. 1967 Sep 5;5(5):1304–1322. doi: 10.1097/00007890-196709000-00009. [DOI] [PubMed] [Google Scholar]
  14. Liacopoulos P., Merchant B., Harrell B. E. Effect of donor immunization with somatic polysaccharides on the graft-vs-host reactivity of transferred donor splenocytes. Proc Soc Exp Biol Med. 1967 Jul;125(3):958–962. doi: 10.3181/00379727-125-32249. [DOI] [PubMed] [Google Scholar]
  15. Lonai P., Feldman M. Cooperation of lymphoid cells in an in vitro graft reaction system. The role of the thymus cell. Transplantation. 1970 Nov;10(5):372–381. doi: 10.1097/00007890-197011000-00003. [DOI] [PubMed] [Google Scholar]
  16. Lubaroff D. M., Waksman B. H. Bone marrow as a source of cells in reactions of cellular hypersensitivity. I. Passive transfer of tuberculin sensitivity in syngeneic systems. J Exp Med. 1968 Dec 1;128(6):1425–1435. doi: 10.1084/jem.128.6.1425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. NEVEU T. ETUDE DE LA COMP'ETITION DES ANTIG'ENES CHEZ LE COBAYE ADULTE. EFFET SUR L'INDUCTION DE L'HYPERSENSIBILIT'E RETARD'EE ET SUR LA FORMATION D'ANTICORPS ENVERS UNE PROT'EINE HOMOLOGUE CONJUGU'EE AVEC LE CHLORURE DE PICRYLE. Ann Inst Pasteur (Paris) 1964 Sep;107:320–339. [PubMed] [Google Scholar]
  19. Schwartz H., Leskowitz S. Antigenic competition in the development of delayed hypersensitivity to the azobenzenearsonate group. Immunochemistry. 1969 May;6(3):503–506. doi: 10.1016/0019-2791(69)90311-5. [DOI] [PubMed] [Google Scholar]

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