Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1971 Jun 1;133(6):1282–1293. doi: 10.1084/jem.133.6.1282

DIFFERENTIAL IMMUNOFLUORESCENCE OF FERTILIZED MOUSE EGGS WITH H-2 AND NON H-2 ANTIBODY

Joy Palm 1, Susan Heyner 1, R L Brinster 1
PMCID: PMC2138925  PMID: 4930569

Abstract

Mouse embryos at the two-cell and blastocyst stages, as well as unfertilized eggs, have been studied by indirect immunofluorescence for the expression of H-2 and non-H-2 histocompatibility antigens on surface membranes. Serologically-specific reactivity to non-H-2 antibody (H-3 and H-6) was observed as diffuse, patchy staining over the entire surface of the blastomeres at the two-cell stage. In contrast, no reactivity of two-cell or unfertilized egg embryos of four inbred strains was observed when antisera containing only multispecific H-2 cytolytic antibody were used. Antisera containing H-2 along with non-H-2 antibody of unknown specificity showed varying degrees of reactivity, which could be shown by absorption studies to be due to the non-H-2 content of the serum. The results suggest that the initial expression of histocompatibility genes varies and support the hypothesis that the appearance of these cell components may relate to specific stages of differentiation.

Full Text

The Full Text of this article is available as a PDF (704.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMOS D. B., ZUMPFT M., ARMSTRONG P. H-5.A AND H-6.A, TWO MOUSE ISOANTIGENS ON RED CELLS AND TISSUES DETECTED SEROLOGICALLY. Transplantation. 1963 Jul;1:270–283. doi: 10.1097/00007890-196301030-00002. [DOI] [PubMed] [Google Scholar]
  2. BILLINGHAM R. E. TRANSPLANTATION IMMUNITY AND THE MATERNAL-FETAL RELATION. N Engl J Med. 1964 Mar 26;270:667–CONTD. doi: 10.1056/NEJM196403262701306. [DOI] [PubMed] [Google Scholar]
  3. Baranska W., Koldovsky P., Koprowski H. Antigenic study of unfertilized mouse eggs: cross reactivity with SV40-induced antigens. Proc Natl Acad Sci U S A. 1970 Sep;67(1):193–199. doi: 10.1073/pnas.67.1.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brinster R. L. Studies on the development of mouse embryos in vitro. IV. Interaction of energy sources. J Reprod Fertil. 1965 Oct;10(2):227–240. doi: 10.1530/jrf.0.0100227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coggin J. H., Ambrose K. R., Anderson N. G. Fetal antigen capable of inducing transplantation immunity against SV40 hamster tumor cells. J Immunol. 1970 Aug;105(2):524–526. [PubMed] [Google Scholar]
  6. Duff R., Rapp F. Reaction of serum from pregnant hamsters with surface of cells transformed by SV40. J Immunol. 1970 Aug;105(2):521–523. [PubMed] [Google Scholar]
  7. EDIDIN M. TRANSPLANTATION ANTIGENS IN THE MOUSE EMBRYO. THE FATE OF EARLY EMBRYO TISSUES TRANSPLANTED TO ADULT HOSTS. J Embryol Exp Morphol. 1964 Jun;12:309–316. [PubMed] [Google Scholar]
  8. Fellous M., Dausset J. Probable heploid expression of HL-A antigens on human spermatozoon. Nature. 1970 Jan 10;225(5228):191–193. doi: 10.1038/225191a0. [DOI] [PubMed] [Google Scholar]
  9. GOODLIN R. C., HERZENBERG L. A. PREGNANCY INDUCED HEMAGGLUTININS TO PATERNAL H-2 ANTIGENS IN MULTIPAROUS MICE. Transplantation. 1964 May;2:357–361. doi: 10.1097/00007890-196405000-00004. [DOI] [PubMed] [Google Scholar]
  10. Gasser D. L. Genetic control of the immune response in mice. I. Segregation data and localization to the fifth linkage group of a gene affecting antibody production. J Immunol. 1969 Jul;103(1):66–70. [PubMed] [Google Scholar]
  11. Gold P., Freedman S. O. Specific carcinoembryonic antigens of the human digestive system. J Exp Med. 1965 Sep 1;122(3):467–481. doi: 10.1084/jem.122.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Goldberg E. H., Aoki T., Boyse E. A., Bennett D. Detection of H-2 antigens on mouse spermatozoa by the cytotoxicity test. Nature. 1970 Nov 7;228(5271):570–572. doi: 10.1038/228570a0. [DOI] [PubMed] [Google Scholar]
  13. Heyner S., Brinster R. L., Palm J. Effect of iso-antibody on pre-implantation mouse embryos. Nature. 1969 May 24;222(5195):783–784. doi: 10.1038/222783a0. [DOI] [PubMed] [Google Scholar]
  14. Hull P. Maternal-foetal incompatibility associated with the H-3 locus in the mouse. Heredity (Edinb) 1969 May;24(2):203–209. doi: 10.1038/hdy.1969.26. [DOI] [PubMed] [Google Scholar]
  15. Kirby D. R., Billington W. D., James D. A. Transplantation of eggs to the kidney and uterus of immunised mice. Transplantation. 1966 Nov;4(6):713–718. doi: 10.1097/00007890-196611000-00006. [DOI] [PubMed] [Google Scholar]
  16. Lilly F. The histocompatibility-2 locus and susceptibility to tumor induction. Natl Cancer Inst Monogr. 1966 Sep;22:631–642. [PubMed] [Google Scholar]
  17. McDevitt H. O., Sela M. Genetic control of the antibody response. I. Demonstration of determinant-specific differences in response to synthetic polypeptide antigens in two strains of inbred mice. J Exp Med. 1965 Sep 1;122(3):517–531. doi: 10.1084/jem.122.3.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Olds P. J. An attempt to detect M-2 antigens on mouse eggs. Transplantation. 1968 May;6(3):478–479. doi: 10.1097/00007890-196805000-00020. [DOI] [PubMed] [Google Scholar]
  19. SCHLESINGER M. SEROLOGIC STUDIES OF EMBRYONIC AND TROPHOBLASTIC TISSUES OF THE MOUSE. J Immunol. 1964 Aug;93:255–263. [PubMed] [Google Scholar]
  20. Sanderson A. R. Quantitative titration, kinetic behaviour, and inhibition of cytotoxic mouse isoantisera. Immunology. 1965 Sep;9(3):287–300. [PMC free article] [PubMed] [Google Scholar]
  21. Simmons R. L. Histoincompatibility and the survival of the fetus: current controversies. Transplant Proc. 1969 Mar;1(1):47–52. [PubMed] [Google Scholar]
  22. TYAN M. L., COLE L. J. Development of transplantation isoantigens in the mouse embryo plus trophoblast. Transplant Bull. 1962 Oct;30:136–140. [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES