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. 1971 Feb 28;133(3):442–453. doi: 10.1084/jem.133.3.442

QUANTITATIVE STUDIES ON THE MIXED LYMPHOCYTE INTERACTION IN RATS

V. TEMPO AND SPECIFICITY OF THE PROLIFERATIVE RESPONSE AND THE NUMBER OF REACTIVE CELLS FROM IMMUNIZED DONORS

Darcy B Wilson 1, Peter C Nowell 1
PMCID: PMC2138939  PMID: 5165123

Abstract

These studies were designed to determine what influence prior immunization with homologous H isoantigens might have on the subsequent proliferative activity of lymphocytes from these animals in the mixed lymphocyte interaction. The results demonstrate the following: (a) Subcutaneous immunization with splenic cells from donors differing at the major H locus accelerates the tempo of the proliferative response to F1 cells bearing these same antigens in the MLI, whereas antigen given systemically reduces the proliferative response. (b) The altered proliferative behavior is specific for the immunizing antigens. (c) The period after immunization during which the MLI displays an altered tempo is a short one, lasting not longer than 3 wk. (d) Whether they are derived from previously immunized or from normal donors, the proportion of lymphocytes responsive in the MLI is the same, even though the response profiles are different. These results suggest that in comparison to immune responses to other types of antigens, immunologic reactivity to the major H isoantigens already involves a large number of antigen-reactive cells in normal animals and that the proportion of these cells is not increased as a result of immunization. Rather, lymphocytes from immunized animals respond more rapidly to the presence of these antigens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ford W. L., Burr W., Simonsen M. A lymph node weight assay for the graft-versus-host activity of rat lymphoid cells. Transplantation. 1970 Sep;10(3):258–266. doi: 10.1097/00007890-197009000-00007. [DOI] [PubMed] [Google Scholar]
  2. Johnston J. M., Wilson D. B. Origin of immunoreactive lymphocytes in rats. Cell Immunol. 1970 Oct;1(4):430–444. doi: 10.1016/0008-8749(70)90019-5. [DOI] [PubMed] [Google Scholar]
  3. MOORHEAD P. S., NOWELL P. C., MELLMAN W. J., BATTIPS D. M., HUNGERFORD D. A. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res. 1960 Sep;20:613–616. doi: 10.1016/0014-4827(60)90138-5. [DOI] [PubMed] [Google Scholar]
  4. PALM J. SEROLOGICAL DETECTION OF HISTOCOMPATIBILITY ANTIGENS IN TWO STRAINS OF RATS. Transplantation. 1964 Sep;2:603–612. doi: 10.1097/00007890-196409000-00001. [DOI] [PubMed] [Google Scholar]
  5. Ramseier H., Palm J. Further studies of histocompatibility loci in rats. Transplantation. 1967 Jul;5(4):721–729. doi: 10.1097/00007890-196707000-00014. [DOI] [PubMed] [Google Scholar]
  6. Silvers W. K., Wilson D. B., Palm J. Mixed leukocyte reactions and histocompatibility in rats. Science. 1967 Feb 10;155(3763):703–704. doi: 10.1126/science.155.3763.703. [DOI] [PubMed] [Google Scholar]
  7. Virolainen M., Häyry P., Defendi V. Effects of presensitization on the mixed lymphocyte reaction of rat spleen cell cultures. Transplantation. 1969 Aug;8(2):179–188. doi: 10.1097/00007890-196908000-00011. [DOI] [PubMed] [Google Scholar]
  8. Wilson D. B., Billingham R. E. Lymphocytes and transplantation immunity. Adv Immunol. 1967;7:189–273. doi: 10.1016/s0065-2776(08)60129-7. [DOI] [PubMed] [Google Scholar]
  9. Wilson D. B., Blyth JL NOWELL P. C. Quantitative studies on the mixed lymphocyte interaction in rats. 3. Kinetics of the response. J Exp Med. 1968 Nov 1;128(5):1157–1181. doi: 10.1084/jem.128.5.1157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wilson D. B., Nowell P. C. Quantitative studies on the mixed lymphocyte interaction in rats. IV. Immunologic potentiality of the responding cells. J Exp Med. 1970 Feb;131(2):391–407. doi: 10.1084/jem.131.2.391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wilson D. B. Quantitative studies on the mixed lymphocyte interaction in rats. I. Conditions and parameters of response. J Exp Med. 1967 Oct 1;126(4):625–654. doi: 10.1084/jem.126.4.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wilson D. B., Silvers W. K., Nowell P. C. Quantitative studies on the mixed lymphocyte interaction in rats. II. Relationship of the proliferative response to the immunologic status of the donors. J Exp Med. 1967 Oct 1;126(4):655–665. doi: 10.1084/jem.126.4.655. [DOI] [PMC free article] [PubMed] [Google Scholar]

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