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. 1971 Feb 28;133(3):572–588. doi: 10.1084/jem.133.3.572

FACTORS INFLUENCING PULMONARY METHANE EXCRETION IN MAN

AN INDIRECT METHOD OF STUDYING THE IN SITU METABOLISM OF THE METHANE-PRODUCING COLONIC BACTERIA

John H Bond Jr 1, Rolf R Engel 1, Michael D Levitt 1
PMCID: PMC2138941  PMID: 5111441

Abstract

Measurements of pulmonary excretion of methane (CH4) were used to obtain information on the CH4-producing bacteria in man. Preliminary studies indicated that (a) all CH4 excreted by man is produced by colonic bacteria, (b) there is no appreciable utilization of CH4 by man, and (c) breath CH4 can serve as a relatively accurate indicator of CH4 production in the intestine. The rate of pulmonary CH4 excretion varied enormously, ranging from undetectable (<5 x 10–6 ml/min) to 0.66 ml/minute. In general, the CH4 excretion rate for subjects was consistently very low (nonproducers) or relatively large (producers). 33.6% of the adult population were producers of CH4. Whereas diet, age over 10 yr, and sex did not influence the rate of CH4 production, some familial factor appeared to play an important role. 84% of siblings of CH4 producers also were producers, while only 18% of the siblings of nonproducers were found to be CH4 producers. This familial tendency appeared to be determined by early environmental rather than genetic factors. These studies of CH4 excretion demonstrate that the exposure of individuals to intestinal bacterial metabolites may differ markedly and that these differences may be chronic and determined by familial factors.

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Selected References

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  1. Bircher J., Müller J., Guggenheim P., Haemmerli U. P. Treatment of chronic portal-systemic encephalopathy with lactulose. Lancet. 1966 Apr 23;1(7443):890–892. doi: 10.1016/s0140-6736(66)91573-x. [DOI] [PubMed] [Google Scholar]
  2. Calloway D. H., Murphy E. L. The use of expired air to measure intestinal gas formation. Ann N Y Acad Sci. 1968 Feb 26;150(1):82–95. doi: 10.1111/j.1749-6632.1968.tb19034.x. [DOI] [PubMed] [Google Scholar]
  3. Coburn R. F., Williams W. J., Kahn S. B. Endogenous carbon monoxide production in patients with hemolytic anemia. J Clin Invest. 1966 Apr;45(4):460–468. doi: 10.1172/JCI105360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DUBOS R., SCHAEDLER R. W., COSTELLO R., HOET P. INDIGENOUS, NORMAL, AND AUTOCHTHONOUS FLORA OF THE GASTROINTESTINAL TRACT. J Exp Med. 1965 Jul 1;122:67–76. doi: 10.1084/jem.122.1.67. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dougherty R. W., O'Toole J. J., Allison M. J. Oxidation of intra-arterially administered carbon 14-labelled methane in sheep. Proc Soc Exp Biol Med. 1967 Apr;124(4):1155–1157. doi: 10.3181/00379727-124-31949. [DOI] [PubMed] [Google Scholar]
  6. FORDTRAN J. S., LEVITAN R., BIKERMAN V., BURROWS B. A., INGELFINGER F. J. The kinetics of water absorption in the human intestine. Trans Assoc Am Physicians. 1961;74:195–206. [PubMed] [Google Scholar]
  7. HUNGATE R. E., FLETCHER D. W., DOUGHERTY R. W., BARRENTINE B. F. Microbial activity in the bovine rumen: its measurement and relation to bloat. Appl Microbiol. 1955 May;3(3):161–173. doi: 10.1128/am.3.3.161-173.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hoskins L. C. Ecological studies of intestinal bacteria. Relation between the specificity of fecal ABO blood group antigen-degrading enzymes from enteric bacteria and the ABO blood group of the human host. J Clin Invest. 1969 Apr;48(4):664–673. doi: 10.1172/JCI106024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KIRK E. The quantity and composition of human colonic flatus. Gastroenterology. 1949 May;12(5):782–794. [PubMed] [Google Scholar]
  10. Levitt M. D. Production and excretion of hydrogen gas in man. N Engl J Med. 1969 Jul 17;281(3):122–127. doi: 10.1056/NEJM196907172810303. [DOI] [PubMed] [Google Scholar]
  11. MURAYAMA M. A MOLECULAR MECHANISM OF SICKLED ERYTHROCYTE FORMATION. Nature. 1964 Apr 18;202:258–260. doi: 10.1038/202258a0. [DOI] [PubMed] [Google Scholar]
  12. Mushin R., Dubos R. Colonization of the mouse intestine with Escherichia coli. J Exp Med. 1965 Oct 1;122(4):745–757. doi: 10.1084/jem.122.4.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nottingham P. M., Hungate R. E. Isolation of methanogenic bacteria from feces of man. J Bacteriol. 1968 Dec;96(6):2178–2179. doi: 10.1128/jb.96.6.2178-2179.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SEARS H. J., BROWNLEE I., UCHIYAMA J. K. Persistence of individual strains of Escherichia coli in the intestinal tract of man. J Bacteriol. 1950 Feb;59(2):293–301. doi: 10.1128/jb.59.2.293-301.1950. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. SMITH P. H., HUNGATE R. E. Isolation and characterization of Methanobacterium ruminantium n. sp. J Bacteriol. 1958 Jun;75(6):713–718. doi: 10.1128/jb.75.6.713-718.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stadtman T. C. Methane fermentation. Annu Rev Microbiol. 1967;21:121–142. doi: 10.1146/annurev.mi.21.100167.001005. [DOI] [PubMed] [Google Scholar]
  17. Zubrzycki L., Spaulding E. H. STUDIES ON THE STABILITY OF THE NORMAL HUMAN FECAL FLORA. J Bacteriol. 1962 May;83(5):968–974. doi: 10.1128/jb.83.5.968-974.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]

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