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. 1971 Feb 28;133(3):602–619. doi: 10.1084/jem.133.3.602

AN EOSINOPHIL LEUKOCYTE CHEMOTACTIC FACTOR OF ANAPHYLAXIS

A B Kay 1, Daniel J Stechschulte 1, K Frank Austen 1
PMCID: PMC2138951  PMID: 4106805

Abstract

The capacity of actively or passively sensitized guinea pig lung to react with antigen to release a factor specifically chemotactic for eosinophil leukocytes (ECF-A) has been demonstrated. The release of ECF-A was also accompanied by the elaboration of both histamine and SRS-A and the appearance of all these mediators exhibited a similar response in terms of the time course of passve sensitization, the effect of antigen dose, the time course of release, divalent cation dependence and enhancement by the presence of succinate or maleate. Decomplementation by the administration of purified cobra venom factor had no effect on the antigen-induced release of ECF-A from actively or passively sensitized lung fragments. When fragments of guinea pig lung were passively sensitized with fractions of guinea pig 7S IgG, only the IgG1-containing fractions prepared tissue for the antigen-induced release of ECF-A. Histamine, SRS-A, bradykinin, serotonin, and the prostaglandins PGE1, PGE2, and PGF2α were not eosinophilotactic per se; neither was ECF-A detected following the incubation of these agents with sensitized lung in the absence of antigen. Both eosinophilotactic activity and SRS-A survived extraction in 80% ethanol and evaporation to dryness. SRS-A, however, withstood boiling in alkaline solution for 20 min, whereas ECF-A activity was abolished by this procedure. SRS-A and ECF-A could also be separated by gel filtration. ECF-A activity was completely recovered following its passage through a column of Sephadex G-25 and had an estimated molecular weight of between 500 and 1000. On the basis of size and a formation mechanism independent of the complement system, ECF-A is distinguishable from a previously described complement-dependent eosinophilotactic factor (ECF-C). Thus, ECF-A represents a hitherto undescribed agent which selectively attracts eosinophil leukocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AUSTEN K. F., BROCKLEHURST W. E. Anaphylaxis in chopped guinea pig lung. II. Enhancement of the anaphylactic release of histamine and slow reacting substance by certain dibasic aliphatic acids and inhibition by monobasic fatty acids. J Exp Med. 1961 Mar 1;113:541–557. doi: 10.1084/jem.113.3.541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. AUSTEN K. F., BROCKLEHURST W. E. Inhibition of the anaphylactic release of histamine from chopped guinea pig lung by chymotrypsin substrates and inhibitors. Nature. 1960 Jun 11;186:866–868. doi: 10.1038/186866a0. [DOI] [PubMed] [Google Scholar]
  3. BAKER A. R., BLOCH K. J., AUSTEN K. F. IN VITRO PASSIVE SENSITIZATION OF CHOPPED GUINEA PIG LUNG BY GUINEA PIG 7S ANTIBODIES. J Immunol. 1964 Oct;93:525–531. [PubMed] [Google Scholar]
  4. BENACERRAF B., LEVINE B. B. Immunological specificity of delayed and immediate hypersensitivity reactions. J Exp Med. 1962 May 1;115:1023–1036. doi: 10.1084/jem.115.5.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. BENACERRAF B., OVARY Z., BLOCH K. J., FRANKLIN E. C. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies. J Exp Med. 1963 Jun 1;117:937–949. doi: 10.1084/jem.117.6.937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BLOCH K. J., KOURILSKY F. M., OVARY Z., BENACERRAF B. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis. J Exp Med. 1963 Jun 1;117:965–981. doi: 10.1084/jem.117.6.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. BROCKLEHURST W. E. The release of histamine and formation of a slow-reacting substance (SRS-A) during anaphylactic shock. J Physiol. 1960 Jun;151:416–435. doi: 10.1113/jphysiol.1960.sp006449. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ballow M., Cochrane C. G. Two anticomplementary factors in cobra venom: hemolysis of guinea pig erythrocytes by one of them. J Immunol. 1969 Nov;103(5):944–952. [PubMed] [Google Scholar]
  9. Jonasson O., Becker E. L. Release of kallikrein from guinea pig lung during anaphylaxis. J Exp Med. 1966 Mar 1;123(3):509–522. doi: 10.1084/jem.123.3.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kay A. B. Studies on eosinophil leucocyte migration. II. Factors specifically chemotactic for eosinophils and neutrophils generated from guinea-pig serum by antigen-antibody complexes. Clin Exp Immunol. 1970 Nov;7(5):723–737. [PMC free article] [PubMed] [Google Scholar]
  11. MONGAR J. L., SCHILD H. O. Inhibition of the anaphylactic reaction. J Physiol. 1957 Feb 15;135(2):301–319. doi: 10.1113/jphysiol.1957.sp005712. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MONGAR J. L., SCHILD H. O. The effect of calcium and pH on the anaphylactic reaction. J Physiol. 1958 Feb 17;140(2):272–284. doi: 10.1113/jphysiol.1958.sp005933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Muller-Eberhard H. J., Nilsson U. R., Dalmasso A. P., Polley M. J., Calcott M. A. A molecular concept of immune cytolysis. Arch Pathol. 1966 Sep;82(3):205–217. [PubMed] [Google Scholar]
  14. Orange R. P., Austen K. F. Slow reacting substance of anaphylaxis. Adv Immunol. 1969;10:105–144. doi: 10.1016/s0065-2776(08)60416-2. [DOI] [PubMed] [Google Scholar]
  15. Shin H. S., Gewurz H., Snyderman R. Reaction of a cobra venom factor with guinea pig complement and generation of an activity chemotactic for polymorphonuclear leukocytes. Proc Soc Exp Biol Med. 1969 May;131(1):203–207. doi: 10.3181/00379727-131-33840. [DOI] [PubMed] [Google Scholar]
  16. Stechschulte D. J., Austen K. F., Bloch K. J. Antibodies involved in antigen-induced release of slow reacting substance of anaphylaxis (SRS-A) in the guinea pig and rat. J Exp Med. 1967 Jan 1;125(1):127–147. doi: 10.1084/jem.125.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]

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