Abstract
Long-term (chronic) allotype suppression, previously reported only in rabbits, is shown here to occur in at least one strain combination of mice as well. Close to 50% of the offspring of SJL (Igb) males mated to BALB/c (Iga) females immunized against the paternal allotype were found to be suppressed for Ig-1b (γG2a) at 6 months of age. These mice are called "chronically" suppressed. The percentage of offspring in this strain combination suppressed for the paternal allotype at 8 wk of age is the same as that seen in an earlier strain combination tested, [(C57 x BALB/c)F1], in which all mice recover from suppression by 10–12 wk. After 8 wk, two distinct patterns of long-term (chronic) suppression emerge in (SJL x BALB/c)F1 mice: a small number of these mice never produce detectable amounts of Ig-1b throughout their lives, while the majority produce detectable Ig-1b sporadically, sometimes over a period of several weeks, the level of which eventually falls below detectability. Attempts to "cure" suppression by destroying the existent lymphoid population and forcing endogenous repopulation in chronically suppressed animals were unsuccessful. Furthermore, attempts to restore Ig-1b production by injection of cells from syngeneic Iga/Igb donors into irradiated, chronically suppressed recipients were also unsuccessful, although the same cell inocula, when injected into irradiated BALB/c (Iga/Iga) mice produced high levels of gamma globulin carrying the allotype. These results suggest that long-term allotype suppression resulting from perinatal exposure of offspring to specific anti-allotype antibody (anti-Ig-1b), is not due merely to an absence of Ig-1b-producing cells or their progenitors, but appears to be an active process, which dominates physiologically over normal production.
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Selected References
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- Anderson H. R. Allotypic suppression of adult mouse spleen cells. Immunology. 1970 Jul;19(1):169–179. [PMC free article] [PubMed] [Google Scholar]
- DRAY S. Effect of maternal isoantibodies on the quantitative expression of two allelic genes controlling gamma-globulin allotypic specificities. Nature. 1962 Aug 18;195:677–680. doi: 10.1038/195677a0. [DOI] [PubMed] [Google Scholar]
- Dubiski S. Suppression of synthesis of allotypically defined immunoglobulins and compensation by another sub-class of immunoglobulin. Nature. 1967 Jun 24;214(5095):1365–1366. doi: 10.1038/2141365a0. [DOI] [PubMed] [Google Scholar]
- HERZENBERG L. A., WARNER N. L., HERZENBERG L. A. IMMUNOGLOBULIN ISOANTIGENS (ALLOTYPES) IN THE MOUSE. I. GENETICS AND CROSS-REACTIONS OF THE 7S GAMMA-2A-ISOANTIGENS CONTROLLED BY ALLELES AT THE IG-1 LOCUS. J Exp Med. 1965 Mar 1;121:415–438. doi: 10.1084/jem.121.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Herzenberg L. A., Goodlin R. C., Rivera E. C. Immunoglobulin synthesis in mice: suppression by anti-allotype antibody. J Exp Med. 1967 Oct 1;126(4):701–713. doi: 10.1084/jem.126.4.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mage R., Dray S. Persistent altered phenotypic expression of allelic gamma-G-immunoglobulin allotypes in heterozygous rabbits exposed to isoantibodies in fetal and neonatal life. J Immunol. 1965 Sep;95(3):525–535. [PubMed] [Google Scholar]
- OUCHTERLONY O. Antigen-antibody reactions in gels. Acta Pathol Microbiol Scand. 1949;26(4):507–515. doi: 10.1111/j.1699-0463.1949.tb00751.x. [DOI] [PubMed] [Google Scholar]