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. 1987 Nov;169(11):5002–5007. doi: 10.1128/jb.169.11.5002-5007.1987

Metabolism of endogenous trehalose by Streptomyces griseus spores and by spores or cells of other actinomycetes.

M J McBride 1, J C Ensign 1
PMCID: PMC213900  PMID: 3117770

Abstract

The disaccharide trehalose is accumulated as a storage product by spores of Streptomyces griseus. Nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. In contrast, spores rapidly depleted their trehalose pools during the first hours of germination. Extracts of dormant spores contained a high specific activity of the enzyme trehalase. The level of trehalase remained relatively constant during germination or incubation in buffer. Nongerminating spores of Streptomyces viridochromogenes, Streptomyces antibioticus, and Micromonospora echinospora and nongrowing spherical cells of Arthrobacter crystallopoietes and Nocardia corallina also maintained large amounts of trehalose and active trehalase. These trehalose reserves were depleted during spore germination or outgrowth of spherical Arthrobacter and Nocardia cells into rods.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Braña A. F., Méndez C., Díaz L. A., Manzanal M. B., Hardisson C. Glycogen and trehalose accumulation during colony development in Streptomyces antibioticus. J Gen Microbiol. 1986 May;132(5):1319–1326. doi: 10.1099/00221287-132-5-1319. [DOI] [PubMed] [Google Scholar]
  2. Crowe J. H., Crowe L. M., Chapman D. Preservation of membranes in anhydrobiotic organisms: the role of trehalose. Science. 1984 Feb 17;223(4637):701–703. doi: 10.1126/science.223.4637.701. [DOI] [PubMed] [Google Scholar]
  3. ENSIGN J. C., WOLFE R. S. NUTRITIONAL CONTROL OF MORPHOGENESIS IN ARTHROBACTER CRYSTALLOPIETES. J Bacteriol. 1964 Apr;87:924–932. doi: 10.1128/jb.87.4.924-932.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Elbein A. D. The metabolism of alpha,alpha-trehalose. Adv Carbohydr Chem Biochem. 1974;30:227–256. doi: 10.1016/s0065-2318(08)60266-8. [DOI] [PubMed] [Google Scholar]
  5. Ensign J. C. Formation, properties, and germination of actinomycete spores. Annu Rev Microbiol. 1978;32:185–219. doi: 10.1146/annurev.mi.32.100178.001153. [DOI] [PubMed] [Google Scholar]
  6. HILL E. P., SUSSMAN A. S. DEVELOPMENT OF TREHALASE AND INVERTASE ACTIVITY IN NEUROSPORA. J Bacteriol. 1964 Dec;88:1556–1566. doi: 10.1128/jb.88.6.1556-1566.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heinzen R. J., Ensign J. C. Effect of growth substrates on morphology of Nocardia corallina. Arch Microbiol. 1975 May 5;103(3):209–217. doi: 10.1007/BF00436352. [DOI] [PubMed] [Google Scholar]
  8. Hey-Ferguson A., Mitchell M., Elbein A. D. Trehalose metabolism in germinating spores of Streptomyces hygroscopicus. J Bacteriol. 1973 Nov;116(2):1084–1085. doi: 10.1128/jb.116.2.1084-1085.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hey A. E., Elbein A. D. Partial prufication and properties of a trehalase from Streptomyces hygroscopicus. J Bacteriol. 1968 Jul;96(1):105–110. doi: 10.1128/jb.96.1.105-110.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Horikoshi K., Ikeda Y. Trehalase in conidia of Aspergillus oryzae. J Bacteriol. 1966 May;91(5):1883–1887. doi: 10.1128/jb.91.5.1883-1887.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jackson D. P., Chan A. H., Cotter D. A. Utilization of trehalose during Dictyostelium discoideum spore germination. Dev Biol. 1982 Apr;90(2):369–374. doi: 10.1016/0012-1606(82)90386-4. [DOI] [PubMed] [Google Scholar]
  12. Kendrick K. E., Ensign J. C. Sporulation of Streptomyces griseus in submerged culture. J Bacteriol. 1983 Jul;155(1):357–366. doi: 10.1128/jb.155.1.357-366.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Koepsel R., Ensign J. C. Microcycle sporulation of Streptomyces viridochromogenes. Arch Microbiol. 1984 Nov;140(1):9–14. doi: 10.1007/BF00409764. [DOI] [PubMed] [Google Scholar]
  14. McBride M. J., Ensign J. C. Effects of intracellular trehalose content on Streptomyces griseus spores. J Bacteriol. 1987 Nov;169(11):4995–5001. doi: 10.1128/jb.169.11.4995-5001.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nakano M., Sacktor B. Renal trehalase: two subsites at the substrate-binding site. Biochim Biophys Acta. 1984 Nov 23;791(1):45–49. doi: 10.1016/0167-4838(84)90279-6. [DOI] [PubMed] [Google Scholar]
  16. Thevelein J. M. Regulation of trehalose mobilization in fungi. Microbiol Rev. 1984 Mar;48(1):42–59. doi: 10.1128/mr.48.1.42-59.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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