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. 1971 Aug 1;134(2):351–365. doi: 10.1084/jem.134.2.351

TOXIC EFFECTS OF STREPTOCOCCAL M PROTEIN ON PLATELETS AND POLYMORPHONUCLEAR LEUKOCYTES IN HUMAN BLOOD

Edwin H Beachey 1, Gene H Stollerman 1
PMCID: PMC2139042  PMID: 4934500

Abstract

Purified M protein isolated from Group A streptococci produced cytotoxic reactions in normal human blood in vitro. In the presence of M antigen, platelets aggregated, fused, and lysed. Polymorphonuclear leukocytes (PMN) surrounded the platelet aggregates, then became highly vacuolated and lysed. In addition, PMN progressively lost their capacity to phagocytose unrelated bacteria and to migrate in glass capillary pipettes. Platelet-PMN reactions were directly proportional to the type-specific precipitin reactivity of each M preparation and could be removed with homologous M antibody, only. Moreover, the reactivity of M protein was abolished by enzymatic digestion with trypsin, but not with lysozyme, strongly suggesting that cell-wall mucopeptide was not involved. Preliminary studies showed that platelet-PMN reactions require heat-stable and heat-labile serum factors, presumably antibody and complement. It is suggested that cytotoxic determinants are uncovered by the extraction and purification process and are intimately associated with the type-specific M determinant, possibly in a molecular complex.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abdulla E. M., Schwab J. H. Biological properties of streptococcal cell-wall particles. 3. Dermonecrotic reaction to cell-wall mucopeptides. J Bacteriol. 1966 Jan;91(1):374–383. doi: 10.1128/jb.91.1.374-383.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BORN G. V. Aggregation of blood platelets by adenosine diphosphate and its reversal. Nature. 1962 Jun 9;194:927–929. doi: 10.1038/194927b0. [DOI] [PubMed] [Google Scholar]
  3. Bryant R. E., DesPrez R. M., VanWay M. H., Rogers D. E. Studies on human leukocyte motility. I. Effects of alterations in pH, electrolyte concentration, and phagocytosis on leukocyte migration, adhesiveness, and aggregation. J Exp Med. 1966 Sep 1;124(3):483–499. doi: 10.1084/jem.124.3.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dossett J. H., Kronvall G., Williams R. C., Jr, Quie P. G. Antiphagocytic effects of staphylococcal protein A. J Immunol. 1969 Dec;103(6):1405–1410. [PubMed] [Google Scholar]
  5. EKSTEDT R. D., STOLLERMAN G. H. Factors affecting the chain length of group A streptococci. II. Quantative M-anti-M relationships in the long chain test. J Exp Med. 1960 Oct 1;112:687–698. doi: 10.1084/jem.112.4.687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Forsgren A., Sjöquist J. "Protein A" from S. aureus. I. Pseudo-immune reaction with human gamma-globulin. J Immunol. 1966 Dec;97(6):822–827. [PubMed] [Google Scholar]
  7. Fox E. N., Wittner M. K. Antigenicity of the M proteins of group A hemolytic streptococci. IV. Cross-reactivity between serotypes. J Immunol. 1968 Jan;100(1):39–45. [PubMed] [Google Scholar]
  8. Fox E. N., Wittner M. K., Dorfman A. Antigenicity of the M proteins of group A hemolytic streptococci. 3. Antibody responses and cutaneous hypersensitivity in humans. J Exp Med. 1966 Dec 1;124(6):1135–1151. doi: 10.1084/jem.124.6.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fox E. N., Wittner M. K. The multiple molecular structure of the M proteins of group A streptococci. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1118–1125. doi: 10.1073/pnas.54.4.1118. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirata A. A., Terasaki P. I. Cross-reactions between streptococcal M proteins and human transplantation antigens. Science. 1970 May 29;168(3935):1095–1096. doi: 10.1126/science.168.3935.1095. [DOI] [PubMed] [Google Scholar]
  11. Jones J. M., Schwab J. H. Effects of streptococcal cell wall fragments on phagocytosis and tissue culture cells. Infect Immun. 1970 Mar;1(3):232–242. doi: 10.1128/iai.1.3.232-242.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. KANTOR F. S., COLE R. M. A fibrinogen precipitating factor (FPF) of group A streptococci. Proc Soc Exp Biol Med. 1959 Oct;102:146–150. doi: 10.3181/00379727-102-25172. [DOI] [PubMed] [Google Scholar]
  13. KANTOR F. S. FIBRINOGEN PRECIPITATION BY STREPTOCOCCAL M PROTEIN. I. IDENTITY OF THE REACTANTS, AND STOICHIOMETRY OF THE REACTION. J Exp Med. 1965 May 1;121:849–859. doi: 10.1084/jem.121.5.849. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LANCEFIELD R. C. Current knowledge of type-specific M antigens of group A streptococci. J Immunol. 1962 Sep;89:307–313. [PubMed] [Google Scholar]
  15. LAWRENCE H. S. The cellular transfer in humans of delayed cutaneous reactivity to hemolytic streptococci. J Immunol. 1952 Feb;68(2):159–178. [PubMed] [Google Scholar]
  16. LAWRENCE H. S. The transfer in humans of delayed skin sensitivity to streptococcal M substance and to tuberculin with disrupted leucocytes. J Clin Invest. 1955 Feb;34(2):219–230. doi: 10.1172/JCI103075. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pachman L. M., Fox E. N. Cellular and antibody reactions to streptococcal M protein types 1, 3, 6 and 12. J Immunol. 1970 Oct;105(4):898–907. [PubMed] [Google Scholar]
  18. STOLLERMAN G. H., EKSTEDT R. D., COHEN I. R. NATURAL RESISTANCE OF GERM-FREE MICE AND COLOSTRUM-DEPRIVED PIGLETS TO GROUP A STREPTOCOCCI. J Immunol. 1965 Jul;95:131–140. [PubMed] [Google Scholar]
  19. Stollerman G. H. Prospects for a vaccine against group A streptococci: the problem of the immunology of M proteins. Arthritis Rheum. 1967 Jun;10(3):245–255. doi: 10.1002/art.1780100311. [DOI] [PubMed] [Google Scholar]
  20. Swanson J., Hsu K. C., Gotschlich E. C. Electron microscopic studies on streptococci. I. M antigen. J Exp Med. 1969 Nov 1;130(5):1063–1091. doi: 10.1084/jem.130.5.1063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Swift H. F., Wilson A. T., Lancefield R. C. TYPING GROUP A HEMOLYTIC STREPTOCOCCI BY M PRECIPITIN REACTIONS IN CAPILLARY PIPETTES. J Exp Med. 1943 Aug 1;78(2):127–133. doi: 10.1084/jem.78.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Widdowson J. P., Maxted W. R., Grant D. L. The production of opacity in serum by group A streptococci and its relationship withthe presence of M antigen. J Gen Microbiol. 1970 Jun;61(3):343–353. doi: 10.1099/00221287-61-3-343. [DOI] [PubMed] [Google Scholar]

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