Abstract
The effect of challenge by antigen on persistence of clones of antibody-producing cells and on the induction of new clones was investigated through quantitative measurements of idiotypic specificities. In each of nine rabbits idiotypic specificities present in the earliest bleedings were completely replaced after a few months; subsequent changes occurred much more slowly. On a quantitative basis the population of molecules used as immunogen always reacted most effectively with the homologous anti-idiotypic antiserum. Little effect of increased antigen dose on the rate of change of idiotype was observed. Even large amounts of antigen administered every 2 wk caused only gradual changes in idiotypic specificities. This was attributed either to more effective capture of antigen by memory cells, as compared to precursor cells, or to the induction of tolerance in those clones that were not expressed. In two of three rabbits on a monthly injection schedule, the idiotypic specificities identified underwent very slow changes over a period as long as 17 months. Changes occurred more rapidly when antigen was administered every 2 wk. In each of four rabbits investigated, all idiotypic specificities identified before a 5 month rest period were still present afterwards, indicating the survival of essentially all clones of antibody-producing cells during that interval. Quantitative inhibition data indicated that some new clones of cells were initiated.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
- Daugharty H., Hopper J. E., MacDonald A. B., Nisonoff A. Quantitative investigations of idiotypic antibodies. I. Analysis of precipitating antibody populations. J Exp Med. 1969 Nov 1;130(5):1047–1062. doi: 10.1084/jem.130.5.1047. [DOI] [PMC free article] [PubMed] [Google Scholar]
- EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
- Eichmann K., Braun D. G., Feizi T., Krause R. M. The emergence of antibodies with either identical or unrelated individual antigenic specificity during repeated immunizations with streptococcal vaccines. J Exp Med. 1970 Jun 1;131(6):1169–1189. doi: 10.1084/jem.131.6.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GELL P. G., KELUS A. S. ANTI-ANTIBODY OR CLONE-PRODUCT? Nature. 1964 Feb 15;201:687–689. doi: 10.1038/201687a0. [DOI] [PubMed] [Google Scholar]
- Hopper J. E., MacDonald A. B., Nisonoff A. Quantitative investigations of idiotypic antibodies. II. Nonprecipitating antibodies. J Exp Med. 1970 Jan 1;131(1):41–56. doi: 10.1084/jem.131.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jaton J. C., Waterfield M. D., Margolies M. N., Bloch K. J., Haber E. Variation in the primary structure of antibodies during the course of immunization. Biochemistry. 1971 Apr 27;10(9):1583–1587. doi: 10.1021/bi00785a012. [DOI] [PubMed] [Google Scholar]
- MacDonald A. B., Alescio L., Nisonoff A. Biosynthesis of antibody molecules with similar properties during prolonged immunization. Biochemistry. 1969 Jul;8(7):3109–3114. doi: 10.1021/bi00835a062. [DOI] [PubMed] [Google Scholar]
- MacDonald A. B., Nisonoff A. Quantitative investigations of idiotypic antibodies. 3. Persistence and variations of idiotypic specificities during the course of immunization. J Exp Med. 1970 Mar 1;131(3):583–601. doi: 10.1084/jem.131.3.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oudin J., Michel M. Idiotypy of rabbit antibodies. II. Comparison of idiotypy of various kinds of antibodies formed in the same rabbits against Salmonella typhi. J Exp Med. 1969 Sep 1;130(3):619–642. doi: 10.1084/jem.130.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SCHOOLEY J. C. Autoradiographic observations of plasma cell formation. J Immunol. 1961 Mar;86:331–337. [PubMed] [Google Scholar]
- TALIAFERRO W. H., TALMAGE D. W. Antibodies in the rabbit with different rates of metabolic decay. J Infect Dis. 1956 Jul-Aug;99(1):21–33. doi: 10.1093/infdis/99.1.21. [DOI] [PubMed] [Google Scholar]
- WEIGLE W. O. Elimination of antigen-antibody complexes from sera of rabbits. J Immunol. 1958 Sep;81(3):204–213. [PubMed] [Google Scholar]