Skip to main content
PMC home page
The Journal of Experimental Medicine logoLink to The Journal of Experimental Medicine
. 1971 Sep 1;134(3):577–587. doi: 10.1084/jem.134.3.577

REGULATION OF THE IMMUNE RESPONSE

I. DIFFERENTIAL EFFECT OF PASSIVELY ADMINISTERED ANTIBODY ON THE THYMUS-DERIVED AND BONE MARROW-DERIVED LYMPHOCYTES

John W Kappler 1, Michael Hoffmann 1, Richard W Dutton 1
PMCID: PMC2139081  PMID: 15776562

Abstract

The effect of passively transfered antiserum against sheep erythrocytes (SRBC) on the antigen stimulated increase of SRBC-specific plaque-forming cells (anti-SRBC-PFC) and SRBC-specific thymus-derived lymphocytes (SRBC-specific T-cells) in the mouse spleen was examined. A dose of antiserum which severely suppressed the development of anti-SRBC-PFC did not prevent the increase in SRBC-specific T-cells, as measured by their ability to cooperate in the in vitro response to trinitrophenylated (TNP) SRBC. It was shown that the insensitivity of these T-cells to antiserum could not be explained by their low antigen requirement as compared to that of PFC. In the in vivo response of mice to TNP-SRBC, antibody specific for TNP suppressed the appearance of both anti-TNP- and anti-SRBC-PFC. The presence of free SRBC specifically prevented the suppression of the anti-SRBC-PFC. These observations are consistent with opsonization by phagocytic cells as the primary means of the observed suppression of PFC development by antibody.

Full Text

The Full Text of this article is available as a PDF (614.5 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bach J. F., Muller J. Y., Dardenne M. In vivo specific antigen recognition by rosette forming cells. Nature. 1970 Sep 19;227(5264):1251–1252. doi: 10.1038/2271251a0. [DOI] [PubMed] [Google Scholar]
  2. Brenneman L., Singer S. J. The generation of antihapten antibodies with electrophoretically homogeneous L chains. Proc Natl Acad Sci U S A. 1968 May;60(1):258–264. doi: 10.1073/pnas.60.1.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cerottini J. C., Nordin A. A., Brunner K. T. Specific in vitro cytotoxicity of thymus-derived lymphocytes sensitized to alloantigens. Nature. 1970 Dec 26;228(5278):1308–1309. doi: 10.1038/2281308a0. [DOI] [PubMed] [Google Scholar]
  4. Chan E. L., Mishell R. I., Mitchell G. F. Cell interaction in an immune response in vitro: requirement for theta-carrying cells. Science. 1970 Dec 11;170(3963):1215–1217. doi: 10.1126/science.170.3963.1215. [DOI] [PubMed] [Google Scholar]
  5. Claman H. N., Chaperon E. A., Triplett R. F. Thymus-marrow cell combinations. Synergism in antibody production. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1167–1171. doi: 10.3181/00379727-122-31353. [DOI] [PubMed] [Google Scholar]
  6. Cunningham A. J. Studies on the cellular basis of IgM immunological memory. The induction of antibody formation in bone marrow cells by primed spleen cells. Immunology. 1969 Dec;17(6):933–942. [PMC free article] [PubMed] [Google Scholar]
  7. Cunningham A. J. Studies on the cellular basis of IgM immunological memory. Immunology. 1969 May;16(5):621–632. [PMC free article] [PubMed] [Google Scholar]
  8. Feldmann M., Diener E. Antibody-mediated suppression of the immune response in vitro. I. Evidence for a central effect. J Exp Med. 1970 Feb;131(2):247–274. doi: 10.1084/jem.131.2.247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Greaves M. F. Biological effects of anti-immunoglobulins: evidence for immunoglobulin receptors on 'T' and 'B' lymphocytes. Transplant Rev. 1970;5:45–75. doi: 10.1111/j.1600-065x.1970.tb00356.x. [DOI] [PubMed] [Google Scholar]
  10. Greaves M. F., Möller E., Möller G. Studies on antigen-binding cells. II. Relationship to antigen-sensitive cells. Cell Immunol. 1970 Oct;1(4):386–403. doi: 10.1016/0008-8749(70)90016-x. [DOI] [PubMed] [Google Scholar]
  11. Greaves M. F., Möller E. Studies on antigen-binding cells. I. The origin of reactive cells. Cell Immunol. 1970 Oct;1(4):372–385. doi: 10.1016/0008-8749(70)90015-8. [DOI] [PubMed] [Google Scholar]
  12. Henry C., Jerne N. K. Competition of 19S and 7S antigen receptors in the regulation of the primary immune response. J Exp Med. 1968 Jul 1;128(1):133–152. doi: 10.1084/jem.128.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. JERNE N. K., NORDIN A. A. Plaque formation in agar by single antibody-producing cells. Science. 1963 Apr 26;140(3565):405–405. [PubMed] [Google Scholar]
  14. Kettman J., Dutton R. W. An in vitro primary immune response to 2,4,6-trinitrophenyl substituted erythrocytes: response against carrier and hapten. J Immunol. 1970 Jun;104(6):1558–1561. [PubMed] [Google Scholar]
  15. Kettman J., Dutton R. W. Radioresistance of the enhancing effect of cells from carrier-immunized mice in an in vitro primary immune response. Proc Natl Acad Sci U S A. 1971 Apr;68(4):699–703. doi: 10.1073/pnas.68.4.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. MOLLER G. Studies on the mechanism of immunological enhancement of tumor homografts. I. Specificity of immunological enhancement. J Natl Cancer Inst. 1963 Jun;30:1153–1175. [PubMed] [Google Scholar]
  17. MOLLER G., WIGZELL H. ANTIBODY SYNTHESIS AT THE CELLULAR LEVEL. ANTIBODY-INDUCED SUPPRESSION OF 19S AND 7S ANTIBODY RESPONSE. J Exp Med. 1965 Jun 1;121:969–989. doi: 10.1084/jem.121.6.969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Pearlman D. S. The influence of antibodies on immunologic responses. I. The effect on the response to particulate antigen in the rabbit. J Exp Med. 1967 Jul 1;126(1):127–148. doi: 10.1084/jem.126.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  22. Ryder R. J., Schwartz R. S. Immunosuppression by antibody: localization of site of action. J Immunol. 1969 Nov;103(5):970–978. [PubMed] [Google Scholar]
  23. SALVIN S. B., SMITH R. F. Delayed hypersensitivity and the anamnestic response. J Immunol. 1960 May;84:449–457. [PubMed] [Google Scholar]
  24. Sinclair N. R., Lees R. K., Elliott E. V. Role of the Fc fragment in the regulation of the primary immune response. Nature. 1968 Dec 7;220(5171):1048–1049. doi: 10.1038/2201048a0. [DOI] [PubMed] [Google Scholar]
  25. UHR J. W., BAUMANN J. B. Antibody formation. I. The suppression of antibody formation by passively administered antibody. J Exp Med. 1961 May 1;113:935–957. doi: 10.1084/jem.113.5.935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. UHR J. W., BAUMANN J. B. Antibody formation. II. The specific anamnestic antibody response. J Exp Med. 1961 May 1;113:959–970. doi: 10.1084/jem.113.5.959. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Uhr J. W., Möller G. Regulatory effect of antibody on the immune response. Adv Immunol. 1968;8:81–127. doi: 10.1016/s0065-2776(08)60465-4. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

RESOURCES