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. 1972 Feb 29;135(3):443–457. doi: 10.1084/jem.135.3.443

VIRAL AND CELLULAR SURFACE ANTIGENS OF MURINE LEUKEMIAS AND MYELOMAS

SEROLOGICAL ANALYSIS BY IMMUNOELECTRON MICROSCOPY

Tadao Aoki 1, Toshitada Takahashi 1
PMCID: PMC2139133  PMID: 5058232

Abstract

Immunoelectron microscopy (IEM) of mouse cells which were productively infected with murine leukemia virus (MuLV) yielded the following conclusions: See PDF for Structure With two exceptions, the alloantigens H-2, θ, Ly-A, and Ly-B were not present on complete or incomplete virions produced by cells bearing these antigens.The exceptions were H-2k (but not H-2b) and θ both appeared on only a minority of virions and never on more than a small part of the circumference. The incidental discovery of an additional envelope antigen on virions produced by a BALB/c mouse myeloma but lacking from passage A Gross virions distinguishes these two viruses as MuLV subtypes; it also illustrates that IEM can be applied as a primary tool for antigenic analysis, as well as for its usual purpose of finding out where antigens are situated.

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Selected References

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