Abstract
An in vitro proliferative system for immunoglobulin-G-forming plasma cells from the bone marrow of mice was established by the addition of antigenic protein and thymic cells or their homogenate to bone marrow cultures. The promoting activity of the thymus on plasma cells was independent from mouse strain, but it differed in strength with the variations of donor strains. Synthesis of immunoglobulin-G in proliferating plasma cells and its antibody reactivity against the administered antigen were demonstrated by immunocytological analyses.
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Selected References
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- Claman H. N., Chaperon E. A., Triplett R. F. Thymus-marrow cell combinations. Synergism in antibody production. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1167–1171. doi: 10.3181/00379727-122-31353. [DOI] [PubMed] [Google Scholar]
- Globerson A., Auerbach R. Reactivation in vitro of immunocompetence in irradiated mouse spleen. J Exp Med. 1967 Aug 1;126(2):223–234. doi: 10.1084/jem.126.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HERZENBERG L. A., WARNER N. L., HERZENBERG L. A. IMMUNOGLOBULIN ISOANTIGENS (ALLOTYPES) IN THE MOUSE. I. GENETICS AND CROSS-REACTIONS OF THE 7S GAMMA-2A-ISOANTIGENS CONTROLLED BY ALLELES AT THE IG-1 LOCUS. J Exp Med. 1965 Mar 1;121:415–438. doi: 10.1084/jem.121.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- METCALF D. The effect of thymectomy on the lymphoid tissues of the mouse. Br J Haematol. 1960 Jul;6:324–333. doi: 10.1111/j.1365-2141.1960.tb06248.x. [DOI] [PubMed] [Google Scholar]
- Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakane P. K., Pierce G. B., Jr Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens. J Cell Biol. 1967 May;33(2):307–318. doi: 10.1083/jcb.33.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parrott D. V., De Sousa M. A., East J. Thymus-dependent areas in the lymphoid organs of neonatally thymectomized mice. J Exp Med. 1966 Jan 1;123(1):191–204. doi: 10.1084/jem.123.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Teodorescu M., Brucker J. Cell differentiation and pseudodifferentiation in in vitro cultures of spleen. Exp Cell Res. 1969 Jun;55(3):410–413. doi: 10.1016/0014-4827(69)90575-8. [DOI] [PubMed] [Google Scholar]