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. 1987 Nov;169(11):5101–5112. doi: 10.1128/jb.169.11.5101-5112.1987

Regulation of the vir genes of Agrobacterium tumefaciens plasmid pTiC58.

P M Rogowsky 1, T J Close 1, J A Chimera 1, J J Shaw 1, C I Kado 1
PMCID: PMC213914  PMID: 2822665

Abstract

The virulence (vir) region of pTiC58 was screened for promoter activities by using gene fusions to a promoterless lux operon in the broad-host-range vector pUCD615. Active vir fragments contained the strongly acetosyringone-inducible promoters of virB, virC, virD, and virE and the weakly inducible promoters of virA and virG. Identical induction patterns were obtained with freshly sliced carrot disks, suggesting that an inducer is released after plant tissue is wounded. Optimal conditions for vir gene induction were pH 5.7 for 50 microM acetosyringone or sinapic acid. The induction of virB and virE by acetosyringone was strictly dependent on intact virA and virG loci. An increase in the copy number of virG resulted in a proportional, acetosyringone-independent increase in vir gene expression, and a further increase occurred only if an inducing compound and virA were present.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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