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. 1972 Feb 29;135(3):698–710. doi: 10.1084/jem.135.3.698

IMMUNE RESPONSES IN VITRO

V. SUPPRESSION OFγM, γG, ANDγA PLAQUE-FORMING CELL RESPONSES IN CULTURES OF PRIMED MOUSE SPLEEN CELLS BY CLASS-SPECIFIC ANTIBODY TO MOUSE IMMUNOGLOBULINS

Carl W Pierce 1, Susan M Solliday 1, Richard Asofsky 1
PMCID: PMC2139143  PMID: 4536707

Abstract

Suppression of Ig class-specific PFC responses by class-specific antibody to mouse immunoglobulin was studied in cultures of spleen cells from immunized mice. In contrast to cultures from normal mice where anti-µ suppressed responses in all Ig classes, anti-µ had progressively less suppressive effect on γ1 and γ2 responses in cultures from immunized mice with time after immunization. This was most pronounced at 10 days after immunization when anti-µ suppressed γM and γA responses, but had no or slight effect on γ1 or γ2 responses which were still suppressed with anti-γ1 and anti-γ2. These changes in precursor cell susceptibility to anti-µ were antigen specific.

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Selected References

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  1. Ada G. L. Antigen binding cells in tolerance and immunity. Transplant Rev. 1970;5:105–129. doi: 10.1111/j.1600-065x.1970.tb00358.x. [DOI] [PubMed] [Google Scholar]
  2. Bankhurst A. D., Warner N. L. Surface immunoglobulins on mouse lymphoid cells. J Immunol. 1971 Aug;107(2):368–373. [PubMed] [Google Scholar]
  3. Basten A., Miller J. F., Warner N. L., Pye J. Specific inactivation of thymus-derived (T) and non-thymus-derived (B) lymphocytes by 125I-labelled antigen. Nat New Biol. 1971 May 26;231(21):104–106. doi: 10.1038/newbio231104a0. [DOI] [PubMed] [Google Scholar]
  4. Byrt P., Ada G. L. An in vitro reaction between labelled flagellin or haemocyanin and lymphocyte-like cells from normal animals. Immunology. 1969 Oct;17(4):503–516. [PMC free article] [PubMed] [Google Scholar]
  5. Claman H. N., Chaperon E. A. Immunologic complementation between thymus and marrow cells--a model for the two-cell theory of immunocompetence. Transplant Rev. 1969;1:92–113. doi: 10.1111/j.1600-065x.1969.tb00137.x. [DOI] [PubMed] [Google Scholar]
  6. Cooper M. D., Raymond D. A., Peterson R. D., South M. A., Good R. A. The functions of the thymus system and the bursa system in the chicken. J Exp Med. 1966 Jan 1;123(1):75–102. doi: 10.1084/jem.123.1.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cunningham A. J. Studies on the cellular basis of IgM immunological memory. Immunology. 1969 May;16(5):621–632. [PMC free article] [PubMed] [Google Scholar]
  8. Davie J. M., Rosenthal A. S., Paul W. E. Receptors on immunocompetent cells. 3. Specificity and nature of receptors on dinitrophenylated guinea pig albumin- 125 I-binding cells of immunized guinea pigs. J Exp Med. 1971 Aug 1;134(2):517–531. doi: 10.1084/jem.134.2.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Davies A. J. The thymus and the cellular basis of immunity. Transplant Rev. 1969;1:43–91. doi: 10.1111/j.1600-065x.1969.tb00136.x. [DOI] [PubMed] [Google Scholar]
  10. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  11. EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
  12. Greaves M. F. Biological effects of anti-immunoglobulins: evidence for immunoglobulin receptors on 'T' and 'B' lymphocytes. Transplant Rev. 1970;5:45–75. doi: 10.1111/j.1600-065x.1970.tb00356.x. [DOI] [PubMed] [Google Scholar]
  13. Hamaoka T., Kitagawa M., Matsuoka Y., Yamamura Y. Antibody production in mice. I. The analysis of immunological memory. Immunology. 1969 Jul;17(1):55–69. [PMC free article] [PubMed] [Google Scholar]
  14. Hartmann K. U. Induction of a hemolysin response in vitro. II. Influence of the thymus-derived cells during the development of the antibody-producing cells. J Exp Med. 1971 Jun 1;133(6):1325–1333. doi: 10.1084/jem.133.6.1325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hartmann K. U. Induction of a hemolysin response in vitro. Interaction of cells of bone marrow origin and thymic origin. J Exp Med. 1970 Dec 1;132(6):1267–1278. doi: 10.1084/jem.132.6.1267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jerne N. K., Nordin A. A. Plaque Formation in Agar by Single Antibody-Producing Cells. Science. 1963 Apr 26;140(3565):405–405. doi: 10.1126/science.140.3565.405. [DOI] [PubMed] [Google Scholar]
  17. L'age-Stehr J., Herzenberg L. A. Immunological memory in mice. I. Physical separation and partial characterization of memory cells for different immunoglobulin classes from each other and from antibody-producing cells. J Exp Med. 1970 Jun 1;131(6):1093–1108. doi: 10.1084/jem.131.6.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. McConnell I., Munro A., Gurner B. W., Coombs R. R. Studies on actively allergized cells. I. The cyto-dynamics and morphology of rosete-forming lymph node cells in mice and inhibition of rosette-formation with antibody to mouse immunoglobulins. Int Arch Allergy Appl Immunol. 1969;35(3):209–227. [PubMed] [Google Scholar]
  19. Miller J. F., Mitchell G. F. Thymus and antigen-reactive cells. Transplant Rev. 1969;1:3–42. doi: 10.1111/j.1600-065x.1969.tb00135.x. [DOI] [PubMed] [Google Scholar]
  20. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Mäkelä O., Cross A. M. The diversity and specialization of immunocytes. Prog Allergy. 1970;14:145–207. doi: 10.1159/000289379. [DOI] [PubMed] [Google Scholar]
  22. Naor D., Sulitzeanu D. Affinity of radioiodinated bovine serum albumin for lymphoid cells. Binding of I-125-BSA to lymphoid cells of immune mice. Isr J Med Sci. 1969 Mar-Apr;5(2):217–229. [PubMed] [Google Scholar]
  23. Naor D., Sulitzneau D. Binding of radioiodinated bovine serum albumin to mouse spleen cells. Nature. 1967 May 13;214(5089):687–688. doi: 10.1038/214687a0. [DOI] [PubMed] [Google Scholar]
  24. Paul W. E. Functional specificity of antigen-binding receptors of lymphocytes. Transplant Rev. 1970;5:130–166. doi: 10.1111/j.1600-065x.1970.tb00359.x. [DOI] [PubMed] [Google Scholar]
  25. Pierce C. W. Immune responses in vitro. I. Cellular requirements for the immune response by nonprimed and primed spleen cells in vitro. J Exp Med. 1969 Aug 1;130(2):345–364. doi: 10.1084/jem.130.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Pierce C. W., Johnson B. M., Gershon H. E., Asofsky R. Immune responses in vitro. 3. Development of primary gamma-M, gamma-G, and gamma-A plaque-forming cell responses in mouse spleen cell cultures stimulated with heterologous erythrocytes. J Exp Med. 1971 Aug 1;134(2):395–416. doi: 10.1084/jem.134.2.395. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Plotz P. H., Talal N., Asofsky R. Assignment of direct and facilitated hemolytic plaques in mice to specific immunoglobulin classes. J Immunol. 1968 Apr;100(4):744–751. [PubMed] [Google Scholar]
  28. Radcliffe G. N., Axelrad M. A. Immunological memory in vitro. J Exp Med. 1971 Apr 1;133(4):846–856. doi: 10.1084/jem.133.4.846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Raff M. C. Two distinct populations of peripheral lymphocytes in mice distinguishable by immunofluorescence. Immunology. 1970 Oct;19(4):637–650. [PMC free article] [PubMed] [Google Scholar]
  30. Sell S., Park A. B., Nordin A. A. Immunoglobulin classes of antibody-forming cells in mice. I. Localized hemolysis-in-agar plaque-forming cells belonging to five immunoglobulin classes. J Immunol. 1970 Feb;104(2):483–494. [PubMed] [Google Scholar]
  31. Sercarz E. E., Byers V. S. The X-Y-Z scheme of immunocyte maturation. 3. Early IgM memory and the nature of the memory cell. J Immunol. 1967 Apr;98(4):836–843. [PubMed] [Google Scholar]
  32. Siskind G. W., Benacerraf B. Cell selection by antigen in the immune response. Adv Immunol. 1969;10:1–50. doi: 10.1016/s0065-2776(08)60414-9. [DOI] [PubMed] [Google Scholar]
  33. Taylor R. B. Cellular cooperation in the antibody response of mice to two serum albumins: specific function of thymus cells. Transplant Rev. 1969;1:114–149. doi: 10.1111/j.1600-065x.1969.tb00138.x. [DOI] [PubMed] [Google Scholar]
  34. UHR J. W., FINKELSTEIN M. S. Antibody formation. IV. Formation of rapidly and slowly sedimenting antibodies and immunological memory to bacteriophage phi-X 174. J Exp Med. 1963 Mar 1;117:457–477. doi: 10.1084/jem.117.3.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Uhr J. W., Finkelstein M. S. The kinetics of antibody formation. Prog Allergy. 1967;10:37–83. [PubMed] [Google Scholar]
  36. Unanue E. R., Grey H. M., Rabellino E., Campbell P., Schmidtke J. Immunoglobulins on the surface of lymphocytes. II. The bone marrow as the main source of lymphocytes with detectable surface-bound immunoglobulin. J Exp Med. 1971 Jun 1;133(6):1188–1198. doi: 10.1084/jem.133.6.1188. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Vitetta E. S., Baur S., Uhr J. W. Cell surface immunoglobulin. II. Isolation and characterization of immunoglobulin from mouse splenic lymphocytes. J Exp Med. 1971 Jul 1;134(1):242–264. doi: 10.1084/jem.134.1.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Walters C. S., Wigzell H. Demonstration of heavy and light chain antigenic determinants on the cell-bound receptor for antigen. Similarities between membrane-attached and humoral antibodies produced by the same cell. J Exp Med. 1970 Dec 1;132(6):1233–1249. doi: 10.1084/jem.132.6.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Warner N. L., Byrt P., Ada G. L. Blocking of the lymphocyte antigen receptor site with anti-immunoglobulin sera in vitro. Nature. 1970 Jun 6;226(5249):942–943. doi: 10.1038/226942a0. [DOI] [PubMed] [Google Scholar]
  40. Wigzell H. Specific fractionation of immunocompetent cells. Transplant Rev. 1970;5:76–104. doi: 10.1111/j.1600-065x.1970.tb00357.x. [DOI] [PubMed] [Google Scholar]

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