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. 1972 Jun 1;135(6):1247–1258. doi: 10.1084/jem.135.6.1247

IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS

LIV. CLASSIFICATION OF ANTI-I AND ANTI-i SERA INTO GROUPS BASED ON REACTIVITY PATTERNS WITH VARIOUS ANTIGENS RELATED TO THE BLOOD GROUP A, B, H, LEa LEbAND PRECURSOR SUBSTANCES

Ten Feizi 1, Elvin A Kabat 1
PMCID: PMC2139175  PMID: 4112563

Abstract

Quantitative precipitin assays were carried out with eleven anti-I and five anti-i cold agglutinin sera. Their reaction patterns with precursor substances and with certain antigens related to the A, B, H, Lea, and Leb substances revealed at least six types of I and four types of i specificity. All of the I and i determinants appear to be represented in varying amounts on that fraction of the ovarian cyst precursor substance OG which was precipitated by 20% ethanol (OG 20% 2X). Only some of these determinants can be detected in the first periodate oxidation stages of A, B, and H substances, in cow substances, and in hydatid cyst fluid.

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Selected References

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  1. ALLEN P. Z., KABAT E. A. Immunochemical studies on blood groups. XXII. Immunochemical studies on the nondialyzable residue from partially hydrolyzed blood group A, B and O(H) substances (P1 fractions). J Immunol. 1959 Apr;82(4):340–357. [PubMed] [Google Scholar]
  2. Beychok S., Kabat E. A. Optical activity and conformation of carbohydrates. I. Optical rotatory dispersion studies on immunochemically reactive amino sugars and their glycosides, milk oligosaccharides, oligosaccharides of glucose, and blood group substances. Biochemistry. 1965 Dec;4(12):2565–2574. doi: 10.1021/bi00888a004. [DOI] [PubMed] [Google Scholar]
  3. Feizi T., Kabat E. A., Vicari G., Anderson B., Marsh W. L. Immunochemical studies on blood groups. XLVII. The I antigen complex--precursors in the A, B, H, Lea, and leb blood group system--hemagglutination-inhibition studies. J Exp Med. 1971 Jan 1;133(1):39–52. doi: 10.1084/jem.133.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Feizi T., Kabat E. A., Vicari G., Anderson B., Marsh W. L. Immunochemical studies on blood groups.XLIX. The I antigen complex: specificity differences among anti-I sera revealed by quantitative precipitin studies; partial structure of the I determinant specific for one anti-I serum. J Immunol. 1971 Jun;106(6):1578–1592. [PubMed] [Google Scholar]
  5. Feizi T., Schumacher M. Light chain homogeneity of post-infective cold agglutinins. Clin Exp Immunol. 1968 Nov;3(9):923–929. [PMC free article] [PubMed] [Google Scholar]
  6. KABAT E. A. Immunochemical studies on blood groups. XXIX. Action of various oligosaccharides from human milk in inhibiting the cross-reactions of type XIV antipneumococcal sera with partially hydrolyzed blood group substances (P1 fractions). Arch Biochem Biophys. 1962 Sep;Suppl 1:181–186. [PubMed] [Google Scholar]
  7. Lloyd K. O., Kabat E. A. Immunochemical studies on blood groups. XLI. Proposed structures for the carbohydrate portions of blood group A, B, H, Lewis-a, and Lewis-b substances. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1470–1477. doi: 10.1073/pnas.61.4.1470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lloyd K. O., Kabat E. A., Licerio E. [Immunochemical studies on blood groups. 38. Structures and activities of oligosaccharides produced by alkaline degradation of blood-group Lewis-a substance. Proposed structure of the carbohydrate chains of human blood-group A, B, H, Le-a, and Le-b substances]. Biochemistry. 1968 Aug;7(8):2976–2990. doi: 10.1021/bi00848a039. [DOI] [PubMed] [Google Scholar]
  9. MARSH W. L. Anti-i: a cold antibody defining the Ii relationship in human red cells. Br J Haematol. 1961 Apr;7:200–209. doi: 10.1111/j.1365-2141.1961.tb00329.x. [DOI] [PubMed] [Google Scholar]
  10. MORGAN W. T. A contribution to human biochemical genetics; the chemical basis of blood-group specificity. Proc R Soc Lond B Biol Sci. 1960 Feb 2;151:308–347. doi: 10.1098/rspb.1960.0002. [DOI] [PubMed] [Google Scholar]
  11. Roelcke D., Uhlenbruck G. Antigen nomenclature. Vox Sang. 1970 May;18(5):478–479. doi: 10.1111/j.1423-0410.1970.tb01488.x. [DOI] [PubMed] [Google Scholar]
  12. Rosse W. F., Borsos T., Rapp H. J. Cold-reacting antibodies: the enhancement of antibody fixation by the first component of complement (C'1a). J Immunol. 1968 Feb;100(2):259–265. [PubMed] [Google Scholar]
  13. SCHIFFMAN G., KABAT E. A., THOMPSON W. IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS. XXX. CLEAVAGE OF A, B, AND H BLOOD-GROUP SUBSTANCES BY ALKALI. Biochemistry. 1964 Jan;3:113–120. doi: 10.1021/bi00889a018. [DOI] [PubMed] [Google Scholar]
  14. Vicari G., Kabat E. A. Immunochemical studies on blood groups. XLII. Isolation and characterization from ovarian cyst fluid of a blood group substance lacking A, B, H, Lea and Leb specificity. J Immunol. 1969 Apr;102(4):821–825. [PubMed] [Google Scholar]
  15. Vicari G., Kabat E. A. Structures and activities of oligosaccharides produced by alkaline degradation of a blood group substance lacking A, B, H, Le b specificities. Biochemistry. 1970 Aug 18;9(17):3414–3421. doi: 10.1021/bi00819a020. [DOI] [PubMed] [Google Scholar]
  16. WIENER A. S., UNGER L. J., COHEN L., FELDMAN J. Type-specific cold auto-antibodies as a cause of acquired hemolytic anemia and hemolytic transfusion reactions: biologic test with bovine red cells. Ann Intern Med. 1956 Feb;44(2):221–240. doi: 10.7326/0003-4819-44-2-221. [DOI] [PubMed] [Google Scholar]

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