Abstract
Radioautographic study of the binding of GAT-125I to spleen cells of genetic responder and nonresponder mice demonstrates that among mice not injected with antigen all strains have approximately the same number of antigen-binding cells; after injection with antigen the number of antigen-binding cells increases in responders but not in nonresponders. Nonresponders are shown to make antibody after injection with GAT complexed with an immunogenic carrier, demonstrating the presence of potentially functional B cells in responders and nonresponders alike. When incubated in the warm, antigen-binding cells of both responders and nonresponders concentrate antigen at one pole of the cell, forming caps.
Full Text
The Full Text of this article is available as a PDF (378.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Ada G. L. Antigen binding cells in tolerance and immunity. Transplant Rev. 1970;5:105–129. doi: 10.1111/j.1600-065x.1970.tb00358.x. [DOI] [PubMed] [Google Scholar]
- Benacerraf B., McDevitt H. O. Histocompatibility-linked immune response genes. Science. 1972 Jan 21;175(4019):273–279. doi: 10.1126/science.175.4019.273. [DOI] [PubMed] [Google Scholar]
- Davie J. M., Paul W. E. Receptors on immunocompetent cells. II. Specificity and nature of receptors on dinitrophenylated guinea pig albumin- 125 I-binding lymphocytes of normal guinea pigs. J Exp Med. 1971 Aug 1;134(2):495–516. doi: 10.1084/jem.134.2.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green I., Paul W. E., Benacerraf B. The behavior of hapten-poly-L-lysine conjugates as complete antigens in genetic responder and as haptens in nonresponder guinea pigs. J Exp Med. 1966 May 1;123(5):859–879. doi: 10.1084/jem.123.5.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin W. J., Maurer P. H., Benacerraf B. Genetic control of immune responsiveness to a glutamic acid, alanine, tyrosine copolymer in mice. I. Linkage of responsiveness to H-2 genotype. J Immunol. 1971 Sep;107(3):715–718. [PubMed] [Google Scholar]
- Merryman C. F., Maurer P. H. Genetic control of immune response to glutamic acid, alanine, tyrosine copolymers in mice. I. Association of responsiveness to H-2 genotype and specificity of the response. J Immunol. 1972 Jan;108(1):135–141. [PubMed] [Google Scholar]
- Pernis B., Forni L., Amante L. Immunoglobulin spots on the surface of rabbit lymphocytes. J Exp Med. 1970 Nov;132(5):1001–1018. doi: 10.1084/jem.132.5.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pinchuck P., Maurer P. H. Antigenicity of polypeptides (poly alpha amino acids). XV. Studies on the immunogenicity of synthetic polypeptides in mice. J Exp Med. 1965 Oct 1;122(4):665–671. doi: 10.1084/jem.122.4.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Unanue E. R. Antigen-binding cells. I. Their idenification and role in the immune response. J Immunol. 1971 Oct;107(4):1168–1174. [PubMed] [Google Scholar]