Abstract
The binding of sensitized lymphocytes to tumor cells that leads to tumor cell lysis in vitro has been investigated using poly-L-lysine-fixed tumor cell monolayers and lymphocytes obtained from the anatomical site of tumor allograft rejection. The results show that magnesium is an important prerequisite for this interaction and that the extent of lymphocyte-tumor cell binding depends upon temperature as well as pH. Binding can occur in the absence of serum, although serum factors are necessary for the completion of the cytolytic process. The poly-L-lysine technique is applicable to the formation of confluent monolayers with virtually any normal or neoplastic cell type, including those that are otherwise nonadherent to surfaces. Cells immobilized by this technique can be used for the specific immunoabsorption and subsequent recovery of effector lymphocytes sensitized against transplantation or tumor cell antigens.
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Selected References
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- Berke G., Levey R. H. Cellular immunoabsorbents in transplantation immunity. Specific in vitro deletion and recovery of mouse lymphoid cells sensitized against allogeneic tumors. J Exp Med. 1972 Apr 1;135(4):972–984. doi: 10.1084/jem.135.4.972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berke G., Sullivan K. A., Amos B. Rejection of ascites tumor allografts. I. Isolation, characterization, and in vitro reactivity of peritoneal lymphoid effector cells from BALB-c mice immune to EL4 leukosis. J Exp Med. 1972 Jun 1;135(6):1334–1350. doi: 10.1084/jem.135.6.1334. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berke G., Sullivan K. A., Amos B. Rejection of ascites tumor allografts. II. A pathway for cell-mediated tumor destruction in vitro by peritoneal exudate lymphoid cells. J Exp Med. 1972 Dec 1;136(6):1594–1604. doi: 10.1084/jem.136.6.1594. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bloom B. R. In vitro approaches to the mechanism of cell-mediated immune reactions. Adv Immunol. 1971;13:101–208. doi: 10.1016/s0065-2776(08)60184-4. [DOI] [PubMed] [Google Scholar]
- Brondz B. D., Snegiröva A. E. Interaction of immune lymphocytes with the mixtures of target cells possessing selected specificities of the H-2 immunizing allele. Immunology. 1971 Apr;20(4):457–468. [PMC free article] [PubMed] [Google Scholar]
- Cerottini J. C., Nordin A. A., Brunner K. T. Cellular and humoral response to transplantation antigens. I. Development of alloantibody-forming cells and cytotoxic lymphocytes in the graft-versus-host reaction. J Exp Med. 1971 Aug 1;134(2):553–564. doi: 10.1084/jem.134.2.553. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clark W. R., Berke G., Feldman M., Sarid S. Macromolecular synthesis during the sensitization of rat lymphcytes on mouse fibroblasts in vitro. Immunochemistry. 1971 Jun;8(6):487–498. doi: 10.1016/0019-2791(71)90400-9. [DOI] [PubMed] [Google Scholar]
- Clark W., Kimura A. Immunoreactivity in a cellular immune reaction of lymphocytes preabsorbed on cell monolayers. Nat New Biol. 1972 Feb 23;235(60):236–238. doi: 10.1038/newbio235236a0. [DOI] [PubMed] [Google Scholar]
- Crone M., Koch C., Simonsen M. The elusive T cell receptor. Transplant Rev. 1972;10:36–56. doi: 10.1111/j.1600-065x.1972.tb01538.x. [DOI] [PubMed] [Google Scholar]
- Golstein P., Erik M. D., Svedmyr A. J., Wigzell H. Cells mediating specific in vitro cytotoxicity. I. Detection of receptor-bearing lymphocytes. J Exp Med. 1971 Dec 1;134(6):1385–1402. doi: 10.1084/jem.134.6.1385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Golstein P., Svedmyr E. A., Blomgren H. Specific adsorption of cytotoxic thymus-processed lymphocytes (T cells) on glutaraldehyde-fixed fibroblast monolayers. Eur J Immunol. 1972 Aug;2(4):380–383. doi: 10.1002/eji.1830020418. [DOI] [PubMed] [Google Scholar]
- Kennedy J. C., Axelrad M. A. An improved assay for haemolytic plaque-forming cells. Immunology. 1971 Feb;20(2):253–257. [PMC free article] [PubMed] [Google Scholar]
- Liberti P. A., Maurer P. H., Clark L. G. Antigenicity of polypeptides (poly-alpha-amino acids). Physicochemical studies of a calcium-dependent antigen-antibody reaction. Biochemistry. 1971 Apr 27;10(9):1632–1639. doi: 10.1021/bi00785a021. [DOI] [PubMed] [Google Scholar]
- Lonai P., Wekerle H., Feldman M. Fractionation of specific antigen-reactive cells in an in vitro system of cell-mediated immunity. Nat New Biol. 1972 Feb 23;235(60):235–236. doi: 10.1038/newbio235235a0. [DOI] [PubMed] [Google Scholar]
- Marchalonis J. J., Atwell J. L., Cone R. E. Isolation of surface immunoglobulin from lymphocytes from human and murine thymus. Nat New Biol. 1972 Feb 23;235(60):240–242. doi: 10.1038/newbio235240a0. [DOI] [PubMed] [Google Scholar]
- Marchalonis J. J., Cone R. E., Atwell J. L. Isolation and partial characterization of lymphocyte surface immunoglobulins. J Exp Med. 1972 Apr 1;135(4):956–971. doi: 10.1084/jem.135.4.956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mauel J., Rudolf H., Chapuis B., Brunner K. T. Studies of allograft immunity in mice. II. Mechanism of target cell inactivation in vitro by sensitized lymphocytes. Immunology. 1970 Apr;18(4):517–535. [PMC free article] [PubMed] [Google Scholar]
- PETHICA B. A. The physical chemistry of cell adhesion. Exp Cell Res. 1961;Suppl 8:123–140. doi: 10.1016/0014-4827(61)90344-5. [DOI] [PubMed] [Google Scholar]
- Vitetta E. S., Bianco C., Nussenzweig V., Uhr J. W. Cell surface immunoglobulin. IV. Distribution among thymocytes, bone mrrow cells, and their derived populations. J Exp Med. 1972 Jul 1;136(1):81–93. doi: 10.1084/jem.136.1.81. [DOI] [PMC free article] [PubMed] [Google Scholar]