Abstract
Mice inoculated with brain homogenates from multiple sclerosis (MS) cases showed marked changes in their leukocyte differential counts, with a decrease in per cent polymorphonuclear neutrophils (PMN) and an increase in the per cent lymphocytes. These changes were based upon an absolute decrease in the number of circulating PMN. The decrease in PMN percentages was apparent at 16 hr after infection and persisted for at least 11 months. The factor responsible for the decrease in PMN was (a) recoverable from 12 hr to 8½ months after inoculation, (b) present in human brain homogenate at a concentration of 3 x 1012, and (c) between 25 and 50 nm in diameter. Inoculation of 100 units of factor into mice and subsequent titration showed that the factor had undergone a net increase in the mouse of at least 109-fold. The factor causing the PMN decrease was found in all MS material thus far tested: three brains, one spleen, three sera, and two cerebrospinal fluid (CSF) from nine cases of MS. The factor was not found in normal human material that included two brains, one spleen, two sera, and two CSF.
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Selected References
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- CHANDLER R. L. Encephalopathy in mice produced by inoculation with scrapie brain material. Lancet. 1961 Jun 24;1(7191):1378–1379. doi: 10.1016/s0140-6736(61)92008-6. [DOI] [PubMed] [Google Scholar]
- DUNN T. B. Normal and pathologic anatomy of the reticular tissue in laboratory mice, with a classification and discussion of neoplasms. J Natl Cancer Inst. 1954 Jun;14(6):1281–1433. [PubMed] [Google Scholar]
- Field E. J. Transmission experiments with multiple sclerosis: an interim report. Br Med J. 1966 Sep 3;2(5513):564–565. doi: 10.1136/bmj.2.5513.564. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hotchin J. Virus, cell surface, and self: lymphocytic choriomeningitis of mice. Am J Clin Pathol. 1971 Sep;56(3):333–349. doi: 10.1093/ajcp/56.3.333. [DOI] [PubMed] [Google Scholar]
- Porter D. D., Porter H. G., Deerhake B. B. Immunofluorescence assay for antigen and antibody in lactic dehydrogenase virus infection of mice. J Immunol. 1969 Feb;102(2):431–436. [PubMed] [Google Scholar]
- Vogt P. K. Avian tumor viruses. Adv Virus Res. 1965;11:293–385. doi: 10.1016/s0065-3527(08)60549-7. [DOI] [PubMed] [Google Scholar]