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. 1972 Sep 1;136(3):556–567. doi: 10.1084/jem.136.3.556

IMMUNOLOGICAL RELEASE OF HISTAMINE AND SLOW REACTING SUBSTANCE OF ANAPHYLAXIS FROM HUMAN LUNG

IV. ENHANCEMENT BY CHOLINERGIC AND ALPHA ADRENERGIC STIMULATION

Michael Kaliner 1, Robert P Orange 1, K Frank Austen 1
PMCID: PMC2139264  PMID: 4115132

Abstract

The immunologic release of histamine and slow reacting substance of anaphylaxis (SRS-A) from human lung tissue can be enhanced by stimulation with either alpha adrenergic agents (phenylephrine or norepinephrine in the presence of propranolol) or cholinergic agents (acetylcholine or Carbachol). The finding that atropine prevents cholinergic but not comparable alpha adrenergic enhancement is consistent with the view that cholinergic and alpha adrenergic agonists interact with separate receptor sites on the target cells involved in the immunologic release of chemical mediators. The consistent qualitative relationship between the antigen-induced release of mediators and the level of cyclic adenosine monophosphate (cyclic AMP) as measured by the isolation of 14C-labeled cyclic AMP after incorporation of adenine-14C into the tissues or by the cyclic AMP binding protein assay suggests that changes in the level of this cyclic nucleotide mediate adrenergic modulation of the release of histamine and SRS-A. The addition of 8-bromo-cyclic guanosine monophosphate (cyclic GMP) produces an enhancement of the immunologic release of mediators while dibutyryl cyclic AMP is inhibitory. As cholinergic-induced enhancement was not associated with a measurable change in the levels of cyclic AMP, the possibility is suggested that cyclic GMP may be the intracellular mediator of cholinergic-induced enhancement of the immunologic release of histamine and SRS-A.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ferrendelli J. A., Steiner A. L., McDougal D. B., Jr, Kipnis D. M. The effect of oxotremorine and atropine on cGMP and cAMP levels in mouse cerebral cortex and cerebellum. Biochem Biophys Res Commun. 1970 Nov 25;41(4):1061–1067. doi: 10.1016/0006-291x(70)90193-2. [DOI] [PubMed] [Google Scholar]
  2. George W. J., Polson J. B., O'Toole A. G., Goldberg N. D. Elevation of guanosine 3',5'-cyclic phosphate in rat heart after perfusion with acetylcholine. Proc Natl Acad Sci U S A. 1970 Jun;66(2):398–403. doi: 10.1073/pnas.66.2.398. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gilman A. G. A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970 Sep;67(1):305–312. doi: 10.1073/pnas.67.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kaliner M. A., Orange R. P., Koopman W. J., Austen K. F., Laraia P. J. Cyclic adenosine 3',5'-monophosphate in human lung. Biochim Biophys Acta. 1971 Oct;252(1):160–164. doi: 10.1016/0304-4165(71)90104-8. [DOI] [PubMed] [Google Scholar]
  5. Kay A. B., Austen K. F. The IgE-mediated release of an eosinophil leukocyte chemotactic factor from human lung. J Immunol. 1971 Sep;107(3):899–902. [PubMed] [Google Scholar]
  6. Kuo J. F., Lee T. P., Reyes P. L., Walton K. G., Donnelly T. E., Jr, Greengard P. Cyclic nucleotide-dependent protein kinases. X. An assay method for the measurement of quanosine 3',5'-monophosphate in various biological materials and a study of agents regulating its levels in heart and brain. J Biol Chem. 1972 Jan 10;247(1):16–22. [PubMed] [Google Scholar]
  7. Laraia P. J., Reddy W. Hormonal regulation of myocardial adenosine 3',5'-monophosphate. Biochim Biophys Acta. 1969 Apr 1;177(2):189–195. doi: 10.1016/0304-4165(69)90127-5. [DOI] [PubMed] [Google Scholar]
  8. Miyamoto E., Kuo J. F., Greengard P. Cyclic nucleotide-dependent protein kinases. 3. Purification and properties of adenosine 3',5'-monophosphate-dependent protein kinase from bovine brain. J Biol Chem. 1969 Dec 10;244(23):6395–6402. [PubMed] [Google Scholar]
  9. Orange R. P., Austen W. G., Austen K. F. Immunological release of histamine and slow-reacting substance of anaphylaxis from human lung. I. Modulation by agents influencing cellular levels of cyclic 3',5'-adenosine monophosphate. J Exp Med. 1971 Sep 1;134(3 Pt 2):136s–148s. [PubMed] [Google Scholar]
  10. Orange R. P., Kaliner M. A., Laraia P. J., Austen K. F. Immunological release of histamine and slow reacting substance of anaphylaxis from human lung. II. Influence of cellular levels of cyclic AMP. Fed Proc. 1971 Nov-Dec;30(6):1725–1729. [PubMed] [Google Scholar]
  11. Parish W. E. Release of histamine and slow reacting substance with mast cell changes after challenge of human lung sensitized passively with reagin in vitro. Nature. 1967 Aug 12;215(5102):738–739. doi: 10.1038/215738a0. [DOI] [PubMed] [Google Scholar]
  12. Sheard P., Killingback P. G., Blair A. M. Antigen induced release of histamine and SRS-A from human lung passively sensitized with reaginic serum. Nature. 1967 Oct 21;216(5112):283–284. doi: 10.1038/216283a0. [DOI] [PubMed] [Google Scholar]

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